Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,096,766	T→C	V29A (GTG→GCG)	rdgB →	dITP/XTP pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,096,766	0	T	C	100.0%	106.4 / NA	30	V29A (GTG→GCG)	rdgB	dITP/XTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/9);  total (21/9)

CAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGT  >  NC_000913/3096638‑3096893                                                                                                                                 |                                                                                                                                caaATCCCGCCAGAAATCGCGGCGCTAAGTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGcc                                                                                                                             <  2:221854/133‑1 (MQ=255)         gCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGa                                                                                                                     >  1:83836/1‑133 (MQ=255)              aaaTCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGCAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCg                                                                                                                <  1:98741/133‑1 (MQ=255)                                        tCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGAGCTCGGCGATGAGTCGGCTGAACAAACCGGc                                                                                      >  1:186225/1‑133 (MQ=255)                                        tCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGc                                                                                      >  1:340517/1‑133 (MQ=255)                                        tCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGCCCCAAACAAACCTCGGGGTTGATTCAGCTGAAGAAACCGGc                                                                                      >  2:208585/1‑133 (MQ=255)                                         ccTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGcc                                                                                     >  1:496166/1‑133 (MQ=255)                                           tATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACGTCGCCGTGGATTCCGCCGAAGTAACCGGCCTg                                                                                   >  2:238919/1‑133 (MQ=255)                                           tATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTAGGCGTTGATTCAGCAGAAGAATCCGGCCTg                                                                                   >  1:293500/1‑133 (MQ=255)                                             tGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGGTTCCGCTGGAGAAACCGGCCTGat                                                                                 >  2:194298/1‑132 (MQ=255)                                             tGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAc                                                                                 >  2:125819/1‑133 (MQ=255)                                             tGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCACGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAc                                                                                 >  1:454699/1‑133 (MQ=255)                                              gCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAcc                                                                                >  1:425285/1‑133 (MQ=255)                                              gCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAcc                                                                                >  1:505249/1‑133 (MQ=255)                                              gCAAAAAGTTCTCCTCGCAACCGGCAATGTCGGTAAAGGGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGAcc                                                                                >  2:70186/1‑133 (MQ=255)                                               cAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGTCCTGACCt                                                                               >  1:276869/1‑133 (MQ=255)                                               cAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCt                                                                               >  2:407329/1‑133 (MQ=255)                                                          ccTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAAc                                                                    <  2:276869/133‑1 (MQ=255)                                                           cTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAAcg                                                                   <  2:376247/133‑1 (MQ=255)                                                                aaCCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGAtt                                                              >  2:115403/1‑133 (MQ=255)                                                                           tCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAgcgcg                                                   <  1:407329/133‑1 (MQ=255)                                                                                aaaGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATg                                              >  1:367881/1‑133 (MQ=255)                                                                                  aGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCg                                            <  1:125819/133‑1 (MQ=255)                                                                                    tGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTAACCTTTATCGAAAACGAGATTCTGATAGCGAGCCATGCGGc                                          >  2:226723/1‑133 (MQ=255)                                                                                     gCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCa                                         >  1:97172/1‑133 (MQ=255)                                                                                             tGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGAc                                 <  1:72947/133‑1 (MQ=255)                                                                                                     ctgctTAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAATCGCGCCAGGCGGCAGAAGTGAGCGCTTTAc                         >  1:162882/1‑133 (MQ=255)                                                                                                         ttAGCGACTTCGGTCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCAATGCTGCAAAAGAGACCCCTTTACCgcc                     >  2:291903/1‑131 (MQ=255)                                                                                                                      tCTTGATATCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTAACCGCTTTACCGGCAATTGCCgacgac        >  2:584318/1‑133 (MQ=255)                                                                                                                            tatCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACtctggt  <  2:367881/133‑1 (MQ=255)                                                                                                                            tatCGCGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACtctggt  <  2:454699/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                CAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATTCCGCTGAAGAAACCGGCCTGACCTTTATCGAAAACGCGATTCTGAAAGCGCGCCATGCGGCAAAAGTGACCGCTTTACCGGCAATTGCCGACGACTCTGGT  >  NC_000913/3096638‑3096893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑