Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,119,228	A→G	intergenic (‑116/+369)	yghJ ← / ← glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,119,228	0	A	G	100.0%	130.5 / NA	37	intergenic (‑116/+369)	yghJ/glcA	putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/17);  total (20/17)

CTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAAAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACG  >  NC_000913/3119104‑3119355                                                                                                                             |                                                                                                                                 cTTATTCACTAATAATGCTAGTCACAAAACGATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTg                                                                                                                          <  1:294625/133‑1 (MQ=255)        aTTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTta                                                                                                                   <  2:139334/133‑1 (MQ=255)               aCGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            >  2:424874/1‑133 (MQ=255)               aCGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            <  2:346023/133‑1 (MQ=255)                cGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAGACGACATCATTGGTAGAGAACAGGTGAAATGTATAAGAATa                                                                                                           >  2:183501/1‑133 (MQ=255)                cGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATGGATAGAGAACAGGTGAAATTTATAAGAATa                                                                                                           >  2:355153/1‑133 (MQ=255)                 gCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATaa                                                                                                          <  1:20050/133‑1 (MQ=255)                  cAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            <  2:579933/130‑1 (MQ=255)                  cAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            >  1:579933/1‑130 (MQ=255)                  cAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAAc                                                                                                         >  2:431073/1‑133 (MQ=255)                   aaGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATaa                                                                                                          <  2:441287/131‑1 (MQ=255)                   aaGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATaa                                                                                                          >  1:441287/1‑131 (MQ=255)                    aGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            <  2:89986/128‑1 (MQ=255)                    aGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAAt                                                                                                            >  1:89986/1‑128 (MQ=255)                        aCAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAAccc                                                                                                       <  1:153360/129‑1 (MQ=255)                        aCAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAAccc                                                                                                       >  2:153360/1‑129 (MQ=255)                         cAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCtata                                                                                                  <  1:418044/133‑1 (MQ=255)                               aaTGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGt                                                                                           >  2:345695/1‑133 (MQ=255)                                aTGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTc                                                                                          >  2:616420/1‑133 (MQ=255)                                 tGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCt                                                                                         >  2:131477/1‑133 (MQ=255)                                   ttAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTAt                                                                                       <  1:424874/133‑1 (MQ=255)                                      aaaaaTTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GAt                                                                                             >  2:448104/1‑124 (MQ=255)                                      aaaaaTTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GAt                                                                                             <  1:448104/124‑1 (MQ=255)                                                                 ttATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCaacaaaca                                                         <  2:139112/133‑1 (MQ=255)                                                                         tATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTa                                                 >  1:624470/1‑133 (MQ=255)                                                                                tctAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACAt                                          >  1:624933/1‑133 (MQ=255)                                                                                         ttCCTCCATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAGCAAACAATAAATTATCAACATATCACAACg                                 >  1:195734/1‑133 (MQ=255)                                                                                          tcctccATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATACAGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACAttt                                        <  1:560544/126‑1 (MQ=255)                                                                                          tcctccATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATACAGATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACAttt                                        >  2:560544/1‑126 (MQ=255)                                                                                          tcctccATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGt                                <  1:131477/133‑1 (MQ=255)                                                                                          tcctccATTCTTAAGAAAACGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGt                                >  1:528419/1‑133 (MQ=255)                                                                                            ctccATTCTTAAGAATACGACATCATAGGTAGGGAACAGGTCAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTaa                              <  2:281157/133‑1 (MQ=255)                                                                                            ctccATTCTTAAGAACACGACATCTTTGCTAGAGAACAGGTAAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTaa                              <  2:242744/133‑1 (MQ=255)                                                                                                           aaCGACATCATTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATttgtt               >  2:604559/1‑133 (MQ=255)                                                                                                                   catTGATAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGttt       >  1:548336/1‑133 (MQ=255)                                                                                                                     ttGATAGAGATCAGGCGAAATTTATAAGAATATCCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTa     <  2:208624/133‑1 (MQ=255)                                                                                                                        aTAGAGAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGGAACTATAAAAAATTATTGGTAGTTTTAACg  >  1:181050/1‑133 (MQ=255)                                                                                                                             |                                                                                                                                 CTTATTCATTAATAACGCAAGTGACAAAACAATGTTAAAAAATTCGTAACTGGGAGAAATAGTTTTATGCTTTATCTTCTCTAATAACTTCCTCCATTCTTAAGAAAACGACATCATTGATAGAAAACAGGTGAAATTTATAAGAATAACCCCTATAC‑GATGTCTATCTGGCTATTTTTACGAAATTTCAACAAACAAAAAATTAACAACATTTCACAACGTAACTATATAAATTTGTTGGTAGTTTTAACG  >  NC_000913/3119104‑3119355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑