Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,142,721 T→C Y90C (TAC→TGC)  hybC ← hydrogenase 2 large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,142,7210TC97.0% 110.8 / NA 33Y90C (TAC→TGC) hybChydrogenase 2 large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (16/16);  minor base A (1/0);  total (17/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGC  >  NC_000913/3142596‑3142847
                                                                                                                             |                                                                                                                              
cGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTgcgc                                                                                                                         >  2:389645/1‑133 (MQ=255)
         tggttggGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCgg                                                                                                                <  2:85213/133‑1 (MQ=255)
           gttggGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGaa                                                                                                              <  2:491257/133‑1 (MQ=255)
               ggTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTc                                                                                                          >  2:350027/1‑133 (MQ=255)
               ggTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTGACGGATGAATTGCGCGTTAACCGGAACGTc                                                                                                          >  1:297869/1‑133 (MQ=255)
                 tCAGCTTGCAGTGCAGAAGAGATGTCCACCCAGTCCAGCGCGGAAGGCTGAGAGAAATGAACAATATGGTCATGCGTGGCGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGa                                                                                                        >  2:152647/1‑133 (MQ=255)
                              cagAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGGCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACt                                                                                           >  2:582821/1‑133 (MQ=255)
                                   gTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTg                                                                                      >  1:62828/1‑133 (MQ=255)
                                           cACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATAGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGa                                                                              <  2:62828/133‑1 (MQ=255)
                                            aCCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa                                                                             >  1:60871/1‑133 (MQ=255)
                                             cccAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAAc                                                                            >  1:388361/1‑133 (MQ=255)
                                                   ccAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa                                                                      <  1:350027/133‑1 (MQ=255)
                                                     aGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACa                                                                    >  2:49201/1‑133 (MQ=255)
                                                        gcCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgcg                                                                 >  2:563472/1‑133 (MQ=255)
                                                         ccGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgcgc                                                                <  2:109794/133‑1 (MQ=255)
                                                             aaGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGa                                                            <  1:32661/133‑1 (MQ=255)
                                                                  gATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt                                                       <  1:389645/133‑1 (MQ=255)
                                                                        aaTGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa                                                 <  1:505599/133‑1 (MQ=255)
                                                                          tGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATa                                               <  1:152647/133‑1 (MQ=255)
                                                                             aCAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc                                            <  2:60871/133‑1 (MQ=255)
                                                                                    ggTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGAt                                     <  2:2669/133‑1 (MQ=255)
                                                                                      tCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATAc                                   >  2:491410/1‑133 (MQ=255)
                                                                                       cATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACg                                  <  2:388361/133‑1 (MQ=255)
                                                                                         tGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTCGTATATACGCCACTGATACGtt                                >  1:263216/1‑133 (MQ=255)
                                                                                         tGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGtt                                >  2:565875/1‑133 (MQ=255)
                                                                                           cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAATACAGCGCGGGAGGGGTAGTACATACTCCACATATACGTTGc                              >  2:296729/1‑133 (MQ=255)
                                                                                           cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGc                              >  2:344553/1‑133 (MQ=255)
                                                                                           cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGGACACACGCCACAGATACGTTGc                              >  1:296701/1‑133 (MQ=255)
                                                                                            gtggtgTGCGCAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGca                             <  1:49201/133‑1 (MQ=255)
                                                                                                tctgCGCAGCTAGAAGGATGTTATGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAat                         <  2:297869/131‑1 (MQ=255)
                                                                                                     gcAGCCAGAATGATGTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATcc                    >  1:519803/1‑133 (MQ=255)
                                                                                                               tgatgTTAAGGATGCTTTTCGCGTTAACCGCAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATcgcg          <  1:296729/133‑1 (MQ=255)
                                                                                                                 atgTTACGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATcgcgcg        <  1:563472/133‑1 (MQ=255)
                                                                                                                       cGGATGCATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATcgc  >  1:16115/1‑133 (MQ=255)
                                                                                                                             |                                                                                                                              
CGGAGGCTTTGGTTGGGTCAGCTTGCAGTGCAGAAGTGATGTCCACCCAGTCCAGCGCCGAAAGCTGATAGAAATGAACAATATGGTCATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCACAATCATCCATGCATCGCGCGGATCGC  >  NC_000913/3142596‑3142847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑