Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,143,320	T→C	D282G (GAC→GGC)	hybB ←	hydrogenase 2 membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,143,320	0	T	C	100.0%	147.6 / NA	42	D282G (GAC→GGC)	hybB	hydrogenase 2 membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/18);  total (24/18)

CCTAACAGTGCGCTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATCC  >  NC_000913/3143194‑3143442                                                                                                                               |                                                                                                                           ccTAACAGTGCGCTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCgg                                                                                                                      >  2:141450/1‑133 (MQ=255)    aaCAGTGCGCTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCaa                                                                                                                   >  2:609280/1‑133 (MQ=255)         tGCGCTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCt                                                                                                              >  1:578733/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTaa                                                                                                            >  2:142738/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTaa                                                                                                            >  1:378766/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTaa                                                                                                            >  1:412596/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTaa                                                                                                            >  1:475196/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACACCACGGAGGAGAGGCAACCGGCAAACGCTaa                                                                                                            >  1:223422/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACACCACGGAGAAGAAGCCACCGGCAAACGCTaa                                                                                                            >  1:211069/1‑133 (MQ=255)           cgcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACAACACGGAGTAGAAGCAACCGGCAAACGCTaa                                                                                                            >  1:280688/1‑133 (MQ=255)            gcTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAAc                                                                                                           >  1:37330/1‑133 (MQ=255)                 gTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAg                                                                                                      >  1:508678/1‑133 (MQ=255)                        cAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTcg                                                                                               <  2:37330/133‑1 (MQ=255)                        cAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTcg                                                                                               <  1:59026/133‑1 (MQ=255)                            aaCAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCgat                                                                                           >  1:538175/1‑133 (MQ=255)                                gCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGTAAACGCTCACGTCAACTTGTCACgatagat                                                                                       >  1:157970/1‑133 (MQ=255)                                 cATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCgatagata                                                                                      >  1:62103/1‑133 (MQ=255)                                   tGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAg                                                                                    >  1:91899/1‑133 (MQ=255)                                     cGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCt                                                                                  <  1:477016/133‑1 (MQ=255)                                     cGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCt                                                                                  >  2:477016/1‑133 (MQ=255)                                                  gCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCgcag                                                                     >  1:30263/1‑133 (MQ=255)                                                    aGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCgcagca                                                                   >  2:132427/1‑133 (MQ=255)                                                      cTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACg                                                                 >  1:621346/1‑133 (MQ=255)                                                       ttCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGa                                                                <  2:30263/133‑1 (MQ=255)                                                                cGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCg                                                       <  1:572882/133‑1 (MQ=255)                                                                   aGAACGACCAGCGGGCAGAGCAGCAGCAGGTCTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCa                                                    <  1:172829/133‑1 (MQ=255)                                                                     aaCGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGc                                                  <  1:423377/133‑1 (MQ=255)                                                                     aaCGACCAGCGGGAACAGCATCAGTAGGTCTTCAATCCAGACCATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGc                                                  <  2:223422/133‑1 (MQ=255)                                                                      aCGACCAGCCGGAAGAGCATCAGCAGAACTTCTATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCACCAAAGCGCAGCACGATGAAAATCGCCAGCa                                                 <  2:280688/133‑1 (MQ=255)                                                                       cGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCaa                                                <  1:132427/133‑1 (MQ=255)                                                                       cGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCaa                                                <  1:141450/133‑1 (MQ=255)                                                                         aCCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAaca                                              <  1:609280/133‑1 (MQ=255)                                                                          ccAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAacac                                             <  2:412596/133‑1 (MQ=255)                                                                               gggAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGa                                        >  2:446513/1‑133 (MQ=255)                                                                                         tCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGt                              <  1:142738/133‑1 (MQ=255)                                                                                         tCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGt                              <  1:446513/133‑1 (MQ=255)                                                                                         tCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGt                              <  2:378766/133‑1 (MQ=255)                                                                                          cagcagGACTTCAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTc                             >  1:531057/1‑133 (MQ=255)                                                                                                     cAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTaacaaa                  <  2:578733/133‑1 (MQ=255)                                                                                                     cAATCCAGAACATCACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGAGAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTaacaaa                  >  2:343100/1‑133 (MQ=255)                                                                                                                  cACGGAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCa     >  1:44482/1‑133 (MQ=255)                                                                                                                     ggAGTAGAAGCCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATcc  <  2:538175/133‑1 (MQ=255)                                                                                                                               |                                                                                                                           CCTAACAGTGCGCTCAGTGCTGACAGGAACAGCATGCGGGAATCATTACGCAGCTTCGCCACACGCAGAACGACCAGCGGGAAGAGCATCAGCAGGACTTCAATCCAGAACATCACGGAGTAGAAGTCACCGGCAAACGCTAACGACAGCTTGTCGCGATAGATAAGCTCGCCAAAGCGCAGCACGATGAAAATCGCCAGCAACACACTGATGGTGTTGGTCAGCTTAACAAACAGACTCTTTTCATCC  >  NC_000913/3143194‑3143442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑