Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,480	T→C	L376L (TTG→CTG)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,480	0	T	C	100.0%	100.1 / NA	29	L376L (TTG→CTG)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/17);  total (12/17)

CAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATA  >  NC_000913/3156360‑3156608                                                                                                                         |                                                                                                                                 cAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAgcc                                                                                                                      <  2:23261/133‑1 (MQ=255)   aTTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAgccgc                                                                                                                    <  2:378546/133‑1 (MQ=255)      aGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGt                                                                                                                 <  1:90846/133‑1 (MQ=255)           tGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGtatata                                                                                                            >  1:102891/1‑133 (MQ=255)            gCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATAc                                                                                                           >  1:530151/1‑133 (MQ=255)              cGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAGAATCATGATATTCCGCTGGATGTCAGCCGCCGTATATACGa                                                                                                         >  2:239282/1‑133 (MQ=255)                     ccTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAgccgcc                                                                                                  <  2:145843/133‑1 (MQ=255)                       tctcCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGccgcccg                                                                                                <  1:400211/133‑1 (MQ=255)                           cGACTACGCTCTGGAGGGCAGCGCCATCCCAGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTaa                                                                                            <  1:239282/133‑1 (MQ=255)                                            gCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCaaa                                                                           >  1:518925/1‑133 (MQ=255)                                                         ggCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCgg                                                              <  2:102891/133‑1 (MQ=255)                                                              tGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTGTTACGCCTCAAGCTCTCGTTTTCGGGCAtt                                                         >  1:195738/1‑133 (MQ=255)                                                                   aaaaaaCTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTc                                                    <  2:530151/133‑1 (MQ=255)                                                                     aaaaCTGGAATAGCATGGCATGACTCAAATGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCa                                                  <  2:195738/133‑1 (MQ=255)                                                                     aaaaCTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCa                                                  <  2:590470/133‑1 (MQ=255)                                                                     aaaaCTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCa                                                  <  1:377822/133‑1 (MQ=255)                                                                          tGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACtt                                             <  2:577744/133‑1 (MQ=255)                                                                                  cACGGCATGACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTcac                                     >  2:119420/1‑133 (MQ=255)                                                                                          gACCCAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTc                             >  2:84744/1‑133 (MQ=255)                                                                                            cccAACTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCAc                           <  1:84744/133‑1 (MQ=255)                                                                                                aCTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGa                       <  1:104222/133‑1 (MQ=255)                                                                                                 cTGGGCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAg                      >  2:64316/1‑133 (MQ=255)                                                                                                    ggCGAAATTCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCt                   >  1:41916/1‑133 (MQ=255)                                                                                                     gCGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTg                  >  2:312449/1‑133 (MQ=255)                                                                                                      cGAAAATCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGc                 >  1:586604/1‑133 (MQ=255)                                                                                                           aTCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCgg            <  1:312449/133‑1 (MQ=255)                                                                                                            tCATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt           <  2:41916/133‑1 (MQ=255)                                                                                                             cATGACATTACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg          <  1:64316/133‑1 (MQ=255)                                                                                                                     tACGCTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCata  >  2:509844/1‑133 (MQ=255)                                                                                                                         |                                                                                                                                 CAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATA  >  NC_000913/3156360‑3156608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑