Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 327,519 A→C I405M (ATT→ATG)  betB ← betaine aldehyde dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913327,5190AC93.1% 95.4 / ‑1.7 29I405M (ATT→ATG) betBbetaine aldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (14/13);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCG  >  NC_000913/327397‑327636
                                                                                                                          |                                                                                                                     
tCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGt                                                                                                             >  1:491575/1‑133 (MQ=255)
              gATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAg                                                                                               >  1:91751/1‑133 (MQ=255)
                tCCAGCAACTACCCGATTACAGCTGATGAACGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGt                                                                                             <  1:290681/133‑1 (MQ=255)
                tCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGt                                                                                             <  1:7750/133‑1 (MQ=255)
                    gCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGa                                                                                         >  2:563110/1‑133 (MQ=255)
                      aaaTACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAAt                                                                                       >  1:315101/1‑133 (MQ=255)
                         tACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGa                                                                                    <  1:563110/133‑1 (MQ=255)
                              gCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCa                                                                               >  1:509287/1‑133 (MQ=255)
                               cTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCAc                                                                              <  2:500658/133‑1 (MQ=255)
                                    aGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGcc                                                                         >  1:156027/1‑133 (MQ=255)
                                          tgaatgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGa                                                                   >  2:507213/1‑133 (MQ=255)
                                           gaatgaCGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGAt                                                                  <  1:552017/133‑1 (MQ=255)
                                                 cgcgATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTc                                                            <  2:156027/133‑1 (MQ=255)
                                                    gATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacg                                                         <  1:507213/133‑1 (MQ=255)
                                                      tGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgca                                                       >  1:548543/1‑133 (MQ=255)
                                                      tGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgca                                                       >  2:415149/1‑133 (MQ=255)
                                                      tGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgca                                                       <  2:315101/133‑1 (MQ=255)
                                                      tGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgca                                                       >  1:75701/1‑133 (MQ=255)
                                                       gcgcgcGGCTCAGGTCCGATGTGACGATGCACGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgcac                                                      <  1:168765/133‑1 (MQ=255)
                                                        cgcgcgGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTcacgcacg                                                     <  1:114976/133‑1 (MQ=255)
                                                                  aGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCatat                                           <  2:548543/133‑1 (MQ=255)
                                                                         cTGTCACGATGCCCGCCGTCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCt                                    <  1:259246/133‑1 (MQ=255)
                                                                         cTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCt                                    >  1:488623/1‑133 (MQ=255)
                                                                         cTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCt                                    <  2:491575/133‑1 (MQ=255)
                                                                         cTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACCGGCCCGAAGATCTCTTAACGCACGACGGTCATATCGTCGCt                                    >  2:259246/1‑133 (MQ=255)
                                                                         cTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACCGGCCCGAAGATCACTTCACGCACGAGGGTCGTATCGTCGCt                                    >  2:234306/1‑133 (MQ=255)
                                                                                  tGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGt                           >  2:599130/1‑133 (MQ=255)
                                                                                                          tATCGTTAGCGCGGCGCATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATgc   >  1:502583/1‑133 (MQ=255)
                                                                                                           aTCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATgcg  <  1:599130/133‑1 (MQ=255)
                                                                                                                          |                                                                                                                     
TCGCCCCAGGTGTTGATCCAGCAAATACCCGCTTCCAGCTGATGAATGACGCGATGCGCGCGGTTCAGGTCCGCTGTCACGATGCCCGCCGCCAGGCCGTAGTCGGTATCGTTAGCGCGGCGAATGACTTCGTCTTCCGACTCGTAGGTCAGAATGGACATCACTGGCCCGAAGATCTCTTCACGCACGATGGTCATATCGTCGCTGCAATCGGTGAACACTGTCGGTGCAACCCATGCG  >  NC_000913/327397‑327636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑