Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,332,241 G→A V165V (GTC→GTT)  yhbE ← inner membrane protein YhbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,2410GA97.1% 114.0 / NA 35V165V (GTC→GTT) yhbEinner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/21);  minor base C (0/1);  total (13/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGGCTTTGCCAGAGGGAAGAGCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCG  >  NC_000913/3332112‑3332363
                                                                                                                                 |                                                                                                                          
aGGCTTTGCCAGAGGGAAGAGCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAAcc                                                                                                                         <  2:117253/133‑1 (MQ=255)
       gCCAGAGGGAAGAGCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGa                                                                                                                  >  1:16173/1‑133 (MQ=255)
         cAGAGGGAAGAGCGAAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATa                                                                                                                >  2:382203/1‑133 (MQ=255)
          agagGGAAGAGCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATaa                                                                                                               <  1:392048/133‑1 (MQ=255)
              ggAAGAGCGCAATTGGACATAAAGCGAAAAGTTAAAACAGGTTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTcc                                                                                                           <  2:269094/133‑1 (MQ=255)
                        aaTTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTaa                                                                                                 <  1:7255/133‑1 (MQ=255)
                             tACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGt                                                                                            <  1:382203/133‑1 (MQ=255)
                               cATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGa                                                                                          <  2:376144/133‑1 (MQ=255)
                                  aaaGTGTACAGTAAACACAGGATCGGCGATGACGCCAGCCGCCGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACGCCCCAGGTGTAATCGGTGAGCt                                                                                       <  2:190482/133‑1 (MQ=255)
                                   aaGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCtt                                                                                      <  2:330347/133‑1 (MQ=255)
                                    aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGt                                                                                            >  2:624315/1‑126 (MQ=255)
                                    aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGt                                                                                            <  1:624315/126‑1 (MQ=255)
                                    aGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCttt                                                                                     >  2:126954/1‑133 (MQ=255)
                                        tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATATCTCCCCAGGTGGAATCGGTGAGCTTTGTaa                                                                                 >  1:190482/1‑133 (MQ=255)
                                        tACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTaa                                                                                 <  2:487610/133‑1 (MQ=255)
                                         aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAg                                                                                         >  2:29484/1‑124 (MQ=255)
                                         aCAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAg                                                                                         <  1:29484/124‑1 (MQ=255)
                                               aaaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATAtctc                                                                          >  2:412999/1‑133 (MQ=255)
                                                aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCg                                                                         >  2:45354/1‑133 (MQ=255)
                                                aaaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCg                                                                         >  1:341857/1‑133 (MQ=255)
                                                 aaCAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGa                                                                        <  1:126954/133‑1 (MQ=255)
                                                       aTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGAc                                                                  >  1:434327/1‑133 (MQ=255)
                                                       aTCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACT‑CCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACt                                                                 >  1:349005/1‑133 (MQ=255)
                                                                gAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCTCAGGTGTAATAGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTaa                                                         >  2:421460/1‑133 (MQ=255)
                                                                gAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTaa                                                         <  2:16173/133‑1 (MQ=255)
                                                                            cGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCa                                             <  1:127097/133‑1 (MQ=255)
                                                                              cAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAgg                                           <  2:434327/133‑1 (MQ=255)
                                                                                 tAAAACCTTTAGCGCCACGCCATATCTCCCCCCAACCGTCGACGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCg                                        <  2:167226/133‑1 (MQ=255)
                                                                                   aaaCCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCt                                      <  1:519366/133‑1 (MQ=255)
                                                                                   aaaCCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACT‑CCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTc                                     <  2:349005/133‑1 (MQ=255)
                                                                                                 acgccaTAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATg                        <  1:518403/133‑1 (MQ=255)
                                                                                                   gccaTAACTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGcc                      <  1:45354/133‑1 (MQ=255)
                                                                                                        aaCTCACCCAAACCGTCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTAc                 <  2:40561/133‑1 (MQ=255)
                                                                                                                       tCGCCGCACCCACCCTAAAGATAACTCCTCAGTTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTgcg  <  2:192780/133‑1 (MQ=255)
                                                                                                                       tCGCCGCACCAACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTgcg  >  1:598921/1‑133 (MQ=255)
                                                                                                                                 |                                                                                                                          
AGGCTTTGCCAGAGGGAAGAGCGCAATTGTACATAAAGTGTACAGTAAAAACAGGATCTGCGGTGAGGCCAGCCGACGCAATAAAACCTTTTGCGCCACGCCATAACTCACCCAAACCGTCGCCGCACCGACCCCAAAGATAACTCCCCAGGTGTAATCGGTGAGCTTTGTAAATATCTCGACCAGACTGGTGTTAAAAAACATCACCAGGCCGCTCAGGAGCATCAATGCCCCTACAACCTGAGTGCTGCG  >  NC_000913/3332112‑3332363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑