Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,356,708	T→G	F662V (TTC→GTC)	gltB →	glutamate synthase subunit GltB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,356,708	0	T	G	100.0%	120.8 / NA	34	F662V (TTC→GTC)	gltB	glutamate synthase subunit GltB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/17);  total (17/17)

AAAGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACTTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCAAAGATTATCGTACCGTGATGCTCA  >  NC_000913/3356582‑3356825                                                                                                                               |                                                                                                                      aaaGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCg                                                                                                                 <  1:311927/133‑1 (MQ=255)  aaGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCgt                                                                                                                >  1:430090/1‑133 (MQ=255)  aaGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCgt                                                                                                                >  1:618240/1‑133 (MQ=255)  aaGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCgt                                                                                                                >  1:604404/1‑133 (MQ=255)            gCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGc                                                                                                      >  2:374240/1‑133 (MQ=255)                            agggCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTa                                                                                      <  2:25765/131‑1 (MQ=255)                             tGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTAt                                                                                     <  2:618240/133‑1 (MQ=255)                             tGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTAt                                                                                     <  2:22661/133‑1 (MQ=255)                              ggCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTAtt                                                                                    <  2:68475/133‑1 (MQ=255)                                        gcgATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATAcc                                                                          <  1:65848/133‑1 (MQ=255)                                         cgATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCt                                                                         <  1:529807/133‑1 (MQ=255)                                                      tCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACg                                                            >  2:471245/1‑133 (MQ=255)                                                      tCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACg                                                            >  2:323434/1‑133 (MQ=255)                                                      tCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACg                                                            >  2:274358/1‑133 (MQ=255)                                                      tCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACg                                                            >  2:490991/1‑133 (MQ=255)                                                      tCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACg                                                            >  2:543671/1‑133 (MQ=255)                                                        tGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCt                                                          <  1:374240/133‑1 (MQ=255)                                                                cAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGgccgcc                                                  <  1:353014/133‑1 (MQ=255)                                                                       tGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGa                                           <  1:323434/133‑1 (MQ=255)                                                                        gCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGac                                          <  1:446561/133‑1 (MQ=255)                                                                         cGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGGTGGGCCGCCAGGTAGaca                                         >  1:208259/1‑133 (MQ=255)                                                                         cGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGaca                                         >  1:473346/1‑133 (MQ=255)                                                                         cGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGaca                                         >  1:512099/1‑133 (MQ=255)                                                                         cGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTACCTATGAAACGGTGGGCCGCCGGGTAGaca                                         >  1:167905/1‑133 (MQ=255)                                                                                 tGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCg                                 >  1:59956/1‑133 (MQ=255)                                                                                 tGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCg                                 >  1:408247/1‑133 (MQ=255)                                                                                       catcatCGTGTAAACTGCCAGCGGCCCCCATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATATCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGcc                           <  2:299936/133‑1 (MQ=255)                                                                                        atcttcGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCa                          <  1:461043/133‑1 (MQ=255)                                                                                        atcatcGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCa                          >  1:479510/1‑133 (MQ=255)                                                                                         tcatcGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCaa                         <  1:471245/133‑1 (MQ=255)                                                                                          catcGTCGAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCaaa                        <  2:604404/133‑1 (MQ=255)                                                                                             cgtcgAAACCGCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGATCTGCCTGGAAGACACGCATTCGATTACCAAAGAt                     >  1:299936/1‑133 (MQ=255)                                                                                                       gCCAGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCAAAGATTATCGTACCg           <  1:274358/133‑1 (MQ=255)                                                                                                          aGCGCCCGCGATCCGCACCACGTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCAAAGATTATCGTACCGTGa        <  2:430090/133‑1 (MQ=255)                                                                                                                cgcgATCCGCACCACGTCGCCGTGTTGCTGGGCTTCAGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCAAAGATTATCGTACCGTGATGCTCa  <  2:92461/133‑1 (MQ=255)                                                                                                                               |                                                                                                                      AAAGATCGCCTGCCGGTTCCAGCCCCGATGGCGGTTGGCGCGATCCAGACCCGTCTGGTCGATCAAAGCCTGCGTTGCGATGCCAACATCATCGTCGAAACCGCCAGCGCCCGCGATCCGCACCACTTCGCCGTGTTGCTGGGCTTCGGCGCGACGGCTATTTATCCATACCTTGCCTATGAAACGCTGGGCCGCCTGGTAGACACCCATGCGATTGCCAAAGATTATCGTACCGTGATGCTCA  >  NC_000913/3356582‑3356825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑