Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,480,828	T→C	T112A (ACC→GCC)	kefG ←	protein required for KefB activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,480,828	0	T	C	100.0%	134.8 / NA	41	T112A (ACC→GCC)	kefG	protein required for KefB activity
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/16);  total (25/16)

AAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGTGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGA  >  NC_000913/3480702‑3480955                                                                                                                               |                                                                                                                                aaaTAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAAt                                                                                                                           <  2:538102/133‑1 (MQ=255)      atgatgGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGc                                                                                                                      <  1:475049/133‑1 (MQ=255)      atgatgGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGc                                                                                                                      <  1:367291/133‑1 (MQ=255)       tgatgGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCt                                                                                                                     <  2:387303/133‑1 (MQ=255)        gatgGGACTTAACCAATGCATCCGGCACATGTCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGTCTCGCCGGCGGTAATCACGCTa                                                                                                                    >  1:241869/1‑133 (MQ=255)          tgGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGTGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACg                                                                                                                  >  1:597208/1‑133 (MQ=255)           gggACTTAACCAATGCATCCGGCACATGTCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGc                                                                                                                 <  2:241869/133‑1 (MQ=255)             gACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCa                                                                                                               <  1:142964/133‑1 (MQ=255)              aCTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAg                                                                                                              >  1:100479/1‑133 (MQ=255)              aCTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAg                                                                                                              <  2:100479/133‑1 (MQ=255)                        tGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATTACGGTAGGCCAGTACTTTCCCg                                                                                                    >  1:272759/1‑133 (MQ=255)                        tGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTTCCg                                                                                                    >  1:482142/1‑133 (MQ=255)                        tGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCg                                                                                                    >  1:522430/1‑133 (MQ=255)                            tCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCTGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAg                                                                                                >  2:71604/1‑133 (MQ=255)                            tCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAg                                                                                                >  1:403622/1‑133 (MQ=255)                                  acaTGCCCGCCGCCATTTCAAAGGGGCGCAACACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGtt                                                                                          <  1:244551/133‑1 (MQ=255)                                        ccgccgccgGTTCAACGGTGCGTAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTtcctcc                                                                                    <  1:274466/133‑1 (MQ=255)                                           ccgccAGTTCAAAGGGACGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGTCAGTTGGTTGCCTCCCgg                                                                                 >  1:288840/1‑133 (MQ=255)                                            cgccAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGc                                                                                <  2:418990/133‑1 (MQ=255)                                                       aGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTTCCGGCCCGCTGGCaaa                                                                     >  1:66704/1‑133 (MQ=255)                                                          ggCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAAc                                                                   >  1:69939/1‑132 (MQ=255)                                                          ggCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAAc                                                                   <  2:69939/132‑1 (MQ=255)                                                           gcgcAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCa                                                                 <  1:363558/133‑1 (MQ=255)                                                            cgcAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCAc                                                                >  2:558680/1‑133 (MQ=255)                                                            cgcAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCACTTGGTTTCCTCCCGGCACGCTAGCAAAACCAc                                                                >  2:214624/1‑133 (MQ=255)                                                            cgcAGCACATCGCTCATCGGGCAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGCAATCTCGCGACGCCACTACTGTCCCGACGGGTGGTTTTGTTCCGGCCCGCCGTCAAAACCAc                                                                >  2:301230/1‑133 (MQ=255)                                                            cgcAGCACATAGCTCCTCGGGTAGCGTTACAGGGCGTCATATCGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCAc                                                                <  2:272759/133‑1 (MQ=255)                                                                   caTCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTaa                                                         >  1:426814/1‑133 (MQ=255)                                                                     tCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATa                                                       >  2:104635/1‑133 (MQ=255)                                                                           aTCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGt                                                 >  2:273431/1‑133 (MQ=255)                                                                                gTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGc                                            >  1:471069/1‑133 (MQ=255)                                                                                   gCGATTCAGTGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCAc                                         <  2:597208/133‑1 (MQ=255)                                                                                   gCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCAc                                         >  1:581645/1‑133 (MQ=255)                                                                                   gCGATTAAGCGCGTCAAAAGGGTAACCCCTTCCCGGCTCGCCGGCGGTAATCCCGCTACGCCGTTATTTCCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCAc                                         <  2:325101/133‑1 (MQ=255)                                                                                              cgTCATAACGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAg                              >  2:26522/1‑133 (MQ=255)                                                                                                    aaCGGTAAGCTCTTTCCGGTTCGCCGGCGGTATTCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGAAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCgg                        <  1:77927/133‑1 (MQ=255)                                                                                                    aaCGGTAAGCCCTTTCCGGCCCGCCGGGGGTAACCCCGCTCCGCCAGTACTTCCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCgg                        <  2:403622/133‑1 (MQ=255)                                                                                                    aaCGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCgg                        <  1:26522/133‑1 (MQ=255)                                                                                                    aaCGGTAAGCACTTTCCGGCGCCCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCgg                        <  1:558680/133‑1 (MQ=255)                                                                                                     aCGGTAAGCCCTTTCCGGCACGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCggg                       <  1:153435/133‑1 (MQ=255)                                                                                                     aCGGTAAGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCggg                       <  1:399996/133‑1 (MQ=255)                                                                                                           aGCCCTTTCGGGTTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCta                 <  1:71604/133‑1 (MQ=255)                                                                                                           aGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTTCTTTTCCTCCAGATGGTTTCCTCCCGGCCCGCCGGCAAAACCACGGCTTAATACCCCGTCCACCCACTCTTTCAGCAGCGCCGGGCAGCta                 >  2:77927/1‑133 (MQ=255)                                                                                                           aGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTCCTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCta                 <  1:104635/133‑1 (MQ=255)                                                                                                           aGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCta                 >  1:83154/1‑133 (MQ=255)                                                                                                           aGCACTTTCCGGCTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCta                 >  1:3149/1‑133 (MQ=255)                                                                                                               cTTTCCGGTTCGCCGCCGGTAAGCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAgg             <  1:214624/133‑1 (MQ=255)                                                                                                                       cTCGCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCCGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCCGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAaga     >  1:603612/1‑133 (MQ=255)                                                                                                                          gCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATGAAGAGGa  <  1:273431/133‑1 (MQ=255)                                                                                                                          gCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGa  <  2:3149/133‑1 (MQ=255)                                                                                                                          gCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGa  >  1:396996/1‑133 (MQ=255)                                                                                                                          gCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGa  >  1:376773/1‑133 (MQ=255)                                                                                                                          gCCGGCGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGa  <  2:83154/133‑1 (MQ=255)                                                                                                                               |                                                                                                                                AAATAATGATGGGACTTAACCAATGCATCCGGCACATGCCCGCCGCCAGTTCAAAGGGGCGCAGCACATCGCTCATCGGGTAGCGATTCAGCGCGTCATAACGGTAAGCACTTTCCGGCTCGCCGGTGGTAATCACGCTACGCCAGTACTTTCCCGCCAGTTGGTTTCCTCCCGGCCCGCTGGCAAAACCACGACTTAATACCCGGTCCAGCCACTCTTTCAGTAGCGCCGGGCAGCTATAGGTATAAAGAGGA  >  NC_000913/3480702‑3480955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑