| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,540,301 | G→A | D47N (GAC→AAC) | feoA → | ferrous iron transport protein A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,540,301 | 0 | G | A | 98.4% | 207.2 / NA | 62 | D47N (GAC→AAC) | feoA | ferrous iron transport protein A |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (28/33); total (29/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTA > NC_000913/3540171‑3540430 | cacTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAAc < 1:600687/133‑1 (MQ=255) tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTTATGCGGTACGCGTCGCTCCACCCGGCAAcccc > 2:204950/1‑133 (MQ=255) tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAAcccc > 1:537077/1‑133 (MQ=255) tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAAcccc > 1:335986/1‑133 (MQ=255) tCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGCGGTTCGCGTCGCTCCACACGGCAAcccc > 2:219339/1‑133 (MQ=255) cAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCAt > 1:487379/1‑133 (MQ=255) cAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCAt > 1:133105/1‑133 (MQ=255) gATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTc < 2:42731/133‑1 (MQ=255) aTACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCa > 1:438613/1‑133 (MQ=255) tGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATc < 2:108207/133‑1 (MQ=255) gCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCGCTCAGCGACCCCAATCATATCg > 1:167699/1‑133 (MQ=255) gCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCg > 1:351401/1‑133 (MQ=255) gCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCg > 2:91418/1‑133 (MQ=255) cGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGa > 1:438872/1‑133 (MQ=255) cGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGa > 1:519850/1‑133 (MQ=255) gTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGaa > 1:69690/1‑133 (MQ=255) ggAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAAc < 1:91418/133‑1 (MQ=255) aaaTCACTGGCTTTTCCCGTGAAATCAGCTCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCcg < 2:329890/133‑1 (MQ=255) aaaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCcg < 1:117579/133‑1 (MQ=255) aaaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCcg < 2:40623/133‑1 (MQ=255) aaaTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCcg < 2:80021/133‑1 (MQ=255) aTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTGATATCGAAACCcgtc > 1:229027/1‑133 (MQ=255) aTCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCcgtc > 2:587993/1‑133 (MQ=255) aCTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtg < 1:135439/133‑1 (MQ=255) cTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt < 2:351401/133‑1 (MQ=255) cTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt < 2:487379/133‑1 (MQ=255) cTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt < 1:557218/133‑1 (MQ=255) cTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt < 2:537077/133‑1 (MQ=255) tGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGtgtg < 2:133105/133‑1 (MQ=255) gCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt < 2:357061/130‑1 (MQ=255) gCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCgtgt > 1:357061/1‑130 (MQ=255) tGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGACGAGTGAGCTTGGTATTa > 1:246860/1‑133 (MQ=255) cATATCGCTAAAAACTGCTTTCGCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCt < 1:280307/133‑1 (MQ=255) atatCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTg < 2:438613/133‑1 (MQ=255) atCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGc < 2:438872/133‑1 (MQ=255) aaaaCTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGc > 2:626563/1‑126 (MQ=255) aaaaCTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGc < 1:626563/126‑1 (MQ=255) ttCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGaa < 2:519850/133‑1 (MQ=255) ttCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGaa < 2:499819/133‑1 (MQ=255) tctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAg > 2:459027/1‑131 (MQ=255) tctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAg < 1:459027/131‑1 (MQ=255) ctTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCGTAGTAGaagcggaagcgg > 1:290041/1‑133 (MQ=255) tGGCATGTCACCTGGCTCATCTGTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGtt < 1:222970/133‑1 (MQ=255) gCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTc > 2:419578/1‑133 (MQ=255) cATGTTACCTGGCTCCTCTTTTAATGTGCTGCTCGTCGCTCAACTCGGCAACCCCACTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTcc < 2:185338/133‑1 (MQ=255) gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGt < 2:69690/133‑1 (MQ=255) gTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGt < 1:602494/133‑1 (MQ=255) ggCTCCTCTTTTAATTTGGTGCGCATCGCTCCCCTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACg < 2:246860/133‑1 (MQ=255) ggCTCCTCTTGTAATGTGGTGCGCCTCGTTCCACTCGGCAAACCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACg < 2:229027/133‑1 (MQ=255) cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTCGAAATTGAAGCGGTTTGCTGTAAATACGGTGa > 2:280382/1‑133 (MQ=255) cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATa < 2:413945/127‑1 (MQ=255) cctcTTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATa > 1:413945/1‑127 (MQ=255) ctctTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGAt > 1:10578/1‑133 (MQ=255) ctctTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGAt > 2:98209/1‑133 (MQ=255) ctctTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAAAAGGGGAGGCGGTTTCGTGTTGATACGGTGAt > 2:164958/1‑133 (MQ=255) ccctTTTAAAGTGGTGCGCGTCGCCCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGAt < 2:290041/131‑1 (MQ=255) ctTTTAATGTGGTGCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGGGGAAGCGATTTTCTTTTAATAGGGTGATaa > 1:297055/1‑133 (MQ=255) tggtgCGCGTCGCTCGACGCGGTAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGa < 1:204950/133‑1 (MQ=255) tggtgCGCGTCGCTCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGa < 1:587993/133‑1 (MQ=255) tCCACTCGGCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCAt > 2:409739/1‑133 (MQ=255) cGGCAACCTCATTCACATCGAACCCCGTCGCGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCtt < 1:164958/133‑1 (MQ=255) ggCAACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTa > 1:450751/1‑133 (MQ=255) | CACTCCAGATACTGCGTGGAAAATCACTGGCTTTTCCCGTGAAATCAGCCCGGCATATCGCCAAAAACTGCTTTCTCTTGGCATGTTACCTGGCTCCTCTTTTAATGTGGTGCGCGTCGCTCCACTCGGCGACCCCATTCATATCGAAACCCGTCGTGTGAGCCTGGTATTACGCAAAAAAGATCTGGCCTTATTAGAAGTGGAAGCGGTTTCCTGTTAATACGGTGATAACAACAATGAAAAAATTAACCATTGGCTTA > NC_000913/3540171‑3540430 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |