Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	362,014	G→A	A389V (GCG→GTG)	lacY ←	lactose permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	362,014	0	G	A	100.0%	108.4 / NA	38	A389V (GCG→GTG)	lacY	lactose permease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

TTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAATCGTCG  >  NC_000913/361884‑362140                                                                                                                                   |                                                                                                                               ttGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCAcc                                                                                                                              >  2:482929/1‑133 (MQ=255)    gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCaccacc                                                                                                                           <  1:335348/133‑1 (MQ=255)    gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCaccacc                                                                                                                           >  2:350216/1‑133 (MQ=255)    gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGCGAAGCCCAGCaccacc                                                                                                                           >  1:419570/1‑133 (MQ=255)    gTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAAAACGGAAACTAACGTCAAGCCCTGCaccacc                                                                                                                           >  1:294547/1‑133 (MQ=255)         aTAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGAcc                                                                                                                      <  1:535128/133‑1 (MQ=255)           aaGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCa                                                                                                                    >  1:433118/1‑133 (MQ=255)            aGGCGCTCCCGCCGCATCCGACTTTGATTGTTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAg                                                                                                                   <  1:204597/133‑1 (MQ=255)               cgcTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCAc                                                                                                                <  1:613143/133‑1 (MQ=255)                   cgcgCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGa                                                                                                            >  1:405332/1‑133 (MQ=255)                   cgcgCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGa                                                                                                            >  2:146487/1‑133 (MQ=255)                                aCATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGAACCAGCACCTGATACGCGCCCTGGa                                                                                               >  2:197376/1‑133 (MQ=255)                                     gattgCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCGGCACCACCAGACTCAGCACCAGATACGCGCCCTGGAAACCg                                                                                          >  2:237316/1‑133 (MQ=255)                                      attgCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGa                                                                                         <  2:33157/133‑1 (MQ=255)                                       ttgCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGAt                                                                                        <  1:412146/133‑1 (MQ=255)                                        tgCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATg                                                                                       <  2:58559/133‑1 (MQ=255)                                         gCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGc                                                                                      >  2:92411/1‑133 (MQ=255)                                          cTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCt                                                                                     <  2:433118/133‑1 (MQ=255)                                           ttAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATg                                                                                       >  1:399886/1‑130 (MQ=255)                                           ttAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATg                                                                                       <  2:399886/130‑1 (MQ=255)                                           ttAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCtt                                                                                    >  2:463330/1‑133 (MQ=255)                                                       attcaCCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCCCCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTg                                                                        <  1:57059/133‑1 (MQ=255)                                                       attcaCCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCCCCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTg                                                                        <  1:466776/133‑1 (MQ=255)                                                        ttcaCCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGc                                                                       >  1:88680/1‑133 (MQ=255)                                                         tcaCCTGACGCCGCAACAGGGAAAAGGGCCCGTGGCCGCTACGCGTGAACACGGAAATAAAGGTGAAGCCCAGCCCCCCCAGACCCAGCCCCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGcc                                                                      <  2:213864/133‑1 (MQ=255)                                                         tcaCCTGAAGACGCAGCACGGATAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGcc                                                                      <  1:197376/133‑1 (MQ=255)                                                            ccTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCCCCGTGGAACCCGATGCTTTCATACATATTGCCCGc                                                                   <  2:419570/133‑1 (MQ=255)                                                               gacgacGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAg                                                                >  1:540250/1‑133 (MQ=255)                                                                acgacgCAGCAGGGAAAGCGGGCTTGGGCCGCAAAGCGTGATCACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGt                                                               <  2:294547/133‑1 (MQ=255)                                                                acgacgCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGt                                                               >  1:544247/1‑133 (MQ=255)                                                                                   gggccggggccgCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCg                                            >  1:355965/1‑133 (MQ=255)                                                                                          ggccgCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACAAAAAAATCATCGCCAGTTg                                     >  1:218674/1‑133 (MQ=255)                                                                                               cTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTaa                                >  2:562575/1‑133 (MQ=255)                                                                                               cTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTaa                                >  1:21968/1‑133 (MQ=255)                                                                                                     gTGAACACGGAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGc                          <  2:355965/133‑1 (MQ=255)                                                                                                              gAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGa                 >  1:438391/1‑133 (MQ=255)                                                                                                              gAAATTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGa                 <  1:6204/133‑1 (MQ=255)                                                                                                               aaaTTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGAc                <  1:350216/133‑1 (MQ=255)                                                                                                               aaaTTAAGGTGAAGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGAc                <  2:544247/133‑1 (MQ=255)                                                                                                                           aGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAAtcgt    >  1:451260/1‑133 (MQ=255)                                                                                                                           aGCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAAtcgt    <  1:482929/133‑1 (MQ=255)                                                                                                                            gCCCAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAAtcgtc   >  1:119452/1‑133 (MQ=255)                                                                                                                             cccAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAAtcgtcg  <  2:88680/133‑1 (MQ=255)                                                                                                                             cccAGCACCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAAtcgtcg  <  1:146487/133‑1 (MQ=255)                                                                                                                                   |                                                                                                                               TTGGTCGGATAAGGCGCTCGCGCCGCATCCGACATTGATTGCTTAAGCGACTTCATTCACCTGACGACGCAGCAGGGAAAGCGGGCCGGGGCCGCTAAGCGTGAACACGGAAATTAAGGTGAAGCCCAGCGCCACCAGACCCAGCACCAGATAAGCGCCCTGGAAACCGATGCTTTCATACATATTGCCCGCCAGTACAGACATAAAAATCATCGCCAGTTGCTTAAAGAAGCAGAAACAGACCAGATAAATCGTCG  >  NC_000913/361884‑362140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑