Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,577,903	T→C	H275R (CAC→CGC)	gntR ←	DNA‑binding transcriptional repressor GntR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,577,903	0	T	C	100.0%	120.3 / NA	34	H275R (CAC→CGC)	gntR	DNA‑binding transcriptional repressor GntR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/12);  total (22/12)

CATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCTGCGGATATTCCCGCCGCG  >  NC_000913/3577773‑3578029                                                                                                                                   |                                                                                                                               cATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCgg                                                                                                                              >  2:405937/1‑133 (MQ=255)      tCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAAcc                                                                                                                         >  2:540883/1‑133 (MQ=255)           gtCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCaa                                                                                                                    <  1:381009/133‑1 (MQ=255)              acaGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCg                                                                                                                 >  1:108489/1‑133 (MQ=255)                      gCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCg                                                                                                         >  1:540986/1‑133 (MQ=255)                      gCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCg                                                                                                         >  1:510830/1‑133 (MQ=255)                            aaTACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGa                                                                                                   <  2:342741/133‑1 (MQ=255)                                  cgcCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACtttt                                                                                             >  2:281966/1‑133 (MQ=255)                                      agcagGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACGGCGGAAACAGGCAATCACCATATCGGCAGAGACTTTTAAcc                                                                                         >  2:165508/1‑133 (MQ=255)                                             gTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACg                                                                                  <  2:510830/133‑1 (MQ=255)                                                                  tCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAAcgccgcgcc                                                             >  1:319271/1‑133 (MQ=255)                                                                   ccGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAAcgccgcgccg                                                            >  1:480186/1‑133 (MQ=255)                                                                   ccGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAAcgccgcgcc                                                             >  1:498876/1‑132 (MQ=255)                                                                   ccGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAAcgccgcgcc                                                             <  2:498876/132‑1 (MQ=255)                                                                   ccGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCCCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAAcgccgcgccg                                                            >  2:359467/1‑133 (MQ=255)                                                                    cGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGa                                                           <  2:319271/133‑1 (MQ=255)                                                                      cTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGAcc                                                         >  2:459623/1‑133 (MQ=255)                                                                      cTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGAcc                                                         >  1:355494/1‑133 (MQ=255)                                                                       tCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCg                                                        <  1:405937/133‑1 (MQ=255)                                                                       tCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCg                                                        <  2:108489/133‑1 (MQ=255)                                                                              gcgTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGtc                                                 <  1:540883/133‑1 (MQ=255)                                                                                     cACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTc                                          >  1:325020/1‑133 (MQ=255)                                                                                         cTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTAc                                      >  1:44113/1‑133 (MQ=255)                                                                                         cTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGCCATCATTCGTAc                                      >  1:569156/1‑133 (MQ=255)                                                                                           cGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAg                                    <  1:585939/133‑1 (MQ=255)                                                                                            gCAAGTCGTGGCTCCCTCTCCTCACCAATGCCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGa                                   <  1:165508/133‑1 (MQ=255)                                                                                                    tGGCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACTGCCAGGTAATCGTTGGTACAGAACACGCCa                           >  1:230272/1‑133 (MQ=255)                                                                                                      gCTCCATCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATc                         >  1:570703/1‑133 (MQ=255)                                                                                                           aTCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGTCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCt                    <  1:81172/133‑1 (MQ=255)                                                                                                            tCACCTGACCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCTg                   <  1:404085/133‑1 (MQ=255)                                                                                                                   aCCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCGGGTCATCAATCGTACAGAACACGCCGTCCAGATGCGGatat            >  2:282406/1‑133 (MQ=255)                                                                                                                   aCCAATGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCTGCGGatat            >  2:85194/1‑133 (MQ=255)                                                                                                                        tGTCATGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCTGCGGATATTCccg       >  1:407199/1‑133 (MQ=255)                                                                                                                             tGACCGCGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCCGCCGATATTCCCGCcgcg  >  1:237147/1‑133 (MQ=255)                                                                                                                                   |                                                                                                                               CATTTTCGGTGTCACAGATTCGCCACGAATACGCGCCAGCAGGCGTTCAGCGCCAATACTGCCCATCCGCTCACGCGGCGTCAGCACGCTCGCAAGTCGTGGCTCCATCACCTGACCAATGTCATGACCGTGGAAACCGGCAATCGCCATATCGTCAGGAACTTTTAACCCCAGACGCTGACATTCAAACGCCGCGCCGACCGCCAGGTCATCATTCGTACAGAACACGCCATCCAGCTGCGGATATTCCCGCCGCG  >  NC_000913/3577773‑3578029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑