Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,838,052	T→C	I367T (ATC→ACC)	setC →	putative arabinose exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,838,052	0	T	C	100.0%	123.7 / NA	36	I367T (ATC→ACC)	setC	putative arabinose exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/15);  total (21/15)

TGGTATCGGCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGA  >  NC_000913/3837924‑3838180                                                                                                                                 |                                                                                                                                 tgGTATCGGCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCCGCTCTGTTGACGGAATGATGGGTGAAACCTgg                                                                                                                              >  2:262973/1‑133 (MQ=255)      tCGGCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATTGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTa                                                                                                                         >  2:212778/1‑133 (MQ=255)      tCGGCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTa                                                                                                                         >  1:149744/1‑133 (MQ=255)         gCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCa                                                                                                                      >  1:36962/1‑133 (MQ=255)         gCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCa                                                                                                                      >  1:48413/1‑133 (MQ=255)           aTGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATg                                                                                                                    <  2:359219/133‑1 (MQ=255)              cTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTGTGTTGACGTAATGATGGTTGAAACCCGGAGTTACCATGCgt                                                                                                                 >  2:161523/1‑133 (MQ=255)                        aGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTctggct                                                                                                       >  1:457975/1‑133 (MQ=255)                               gATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATa                                                                                                <  2:595399/133‑1 (MQ=255)                               gATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATa                                                                                                <  2:149744/133‑1 (MQ=255)                                    cTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGAt                                                                                           >  2:337232/1‑133 (MQ=255)                                    cTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGAt                                                                                           >  2:438758/1‑133 (MQ=255)                                       aaaaaaTAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCGTGCGTTGATCTAGCGGGCGATGGGGATGtt                                                                                        >  1:160023/1‑133 (MQ=255)                                       aaaaaaTAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAggg                                                                                             >  2:431085/1‑128 (MQ=255)                                       aaaaaaTAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAggg                                                                                             <  1:431085/128‑1 (MQ=255)                                       aaaaaaTAAGCTCTGCGACAACCTTATATGGAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGtt                                                                                        <  1:161523/133‑1 (MQ=255)                                                    tGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCgatg                                                                           >  1:582471/1‑133 (MQ=255)                                                             gTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCctg                                                                  <  2:439281/133‑1 (MQ=255)                                                               tataTGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCctact                                                                <  2:36962/133‑1 (MQ=255)                                                                     cAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttat                                                          <  1:337232/133‑1 (MQ=255)                                                                     cAAATACTTCACGCGACGGCTGGAATATCGCCCGCTCTGTTTACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttat                                                          <  2:195575/133‑1 (MQ=255)                                                                     cAAATACTGCACGCGTCGTCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttat                                                          <  1:262973/133‑1 (MQ=255)                                                                      aaaTACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttatt                                                         >  2:101625/1‑133 (MQ=255)                                                                      aaaTACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttatt                                                         >  1:245475/1‑133 (MQ=255)                                                                       aaTACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTttatta                                                        >  2:89350/1‑133 (MQ=255)                                                                        aTACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTaa                                                       >  2:436995/1‑133 (MQ=255)                                                                             tCACGCGTCGTCTGGTTTATCGCCTGCTCTGTCGACGGAATCATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGata                                                  <  2:160023/133‑1 (MQ=255)                                                                             tCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGata                                                  <  2:48413/133‑1 (MQ=255)                                                                             tCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTACGATGATTTGCTTGATGTTTATTAAAGata                                                  >  1:195575/1‑133 (MQ=255)                                                                                    tCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCt                                           >  1:155747/1‑133 (MQ=255)                                                                                      ggCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCa                                         <  1:438758/133‑1 (MQ=255)                                                                                                                 ggAATTATTGTTGAACCCAGGATCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGt              <  1:212778/133‑1 (MQ=255)                                                                                                                      tATGGTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGgcgc         >  1:569606/1‑133 (MQ=255)                                                                                                                         ggTTGAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCaaa      <  2:69558/133‑1 (MQ=255)                                                                                                                             gAAACCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACaga  >  2:519839/1‑133 (MQ=255)                                                                                                                             gAAACCTGGAGCTACCATGCGTTGTTCTGGCGGGCGATAGGGATGTTGGGCATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCGCACCAGTCAGGGATAACAAAAATCGGCGTGCCGCAAACaga  >  2:218110/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                 TGGTATCGGCATGCTTTATTTTCAGGACTTGATGCCTGAAAAAATAGGCTCTGCGACAACGTTATATGCAAATACTTCACGCGTCGGCTGGATTATCGCCGGCTCTGTTGACGGAATTATGGTTGAAATCTGGAGCTACCATGCGTTGTTCTGGCTGGCGATAGGGATGTTGGGTATTGCGATGATTTGCCTGCTGTTTATTAAAGATATTTAGCTCACCAGTCTGGGCTAAGAAAAATCGGCGTGGCGCAAACAGA  >  NC_000913/3837924‑3838180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑