Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,846,324	A→G	S282P (TCA→CCA)	uhpT ←	hexose‑6‑phosphate:phosphate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,846,324	0	A	G	94.7%	56.1 / ‑4.4	19	S282P (TCA→CCA)	uhpT	hexose‑6‑phosphate:phosphate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (4/14);  total (4/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

ACCGTTCGCCAGGTCAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTC  >  NC_000913/3846196‑3846453                                                                                                                                 |                                                                                                                                  aCCGTTCGCCAGGTCAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCAc                                                                                                                               <  1:11752/133‑1 (MQ=255)      tCGCCAGGTCAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTc                                                                                                                          <  1:468706/133‑1 (MQ=255)        gCCAGGTCAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGa                                                                                                                        <  2:569683/133‑1 (MQ=255)          cAGGTCAGAGGGCCACCCCCACAGCAGCCTACCGACCAGCGAACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATa                                                                                                                      <  2:169172/133‑1 (MQ=255)               cAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAAt                                                                                                                 <  2:15570/133‑1 (MQ=255)               cAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAAt                                                                                                                 <  2:82296/133‑1 (MQ=255)               cAGAGAGCCAGCCCCACAGCAGCGGACCGACCGGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAAt                                                                                                                 <  2:250897/133‑1 (MQ=255)                    agccagccCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTa                                                                                                            <  1:137821/133‑1 (MQ=255)                           cccACAGCAGTGTACTGACCAGCGTACCGGCTTCAAACAGCCTAAAGCCCTGAATGGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATa                                                                                                     <  2:190134/133‑1 (MQ=255)                              acaGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATagag                                                                                                  >  1:130140/1‑133 (MQ=255)                                                      cAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCAcagcag                                                                          <  2:59382/133‑1 (MQ=255)                                                      cAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCAcagcag                                                                          <  1:443537/133‑1 (MQ=255)                                                                         gCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTc                                                       <  2:130140/133‑1 (MQ=255)                                                                             tGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGca                                                   >  2:417856/1‑133 (MQ=255)                                                                                   gCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACt                                             <  1:417856/133‑1 (MQ=255)                                                                                                agagTTTCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTg                                <  2:414158/133‑1 (MQ=255)                                                                                                     ttCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCa                             <  1:425930/131‑1 (MQ=255)                                                                                                     ttCAGTTCCTGGAACGCGTATACGGTTGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCa                             >  2:425930/1‑131 (MQ=255)                                                                                                                              ttGGCCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGtctc  >  2:146945/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                  ACCGTTCGCCAGGTCAGAGAGCCAGCCCCACAGCAGCGTACCGACCAGCGCACCAGCTTCAAACAGCGTAAAGCCCTGAATCGCCACCGCTTTAGAGAGTTTCAGTTCCTGGAACGCGTATACGGTTGACCACTGGTCGATACCAATACGTACCACATAGAGGAAAATGTTGGCGAAGCACAGCAGCCAGATCACTTTGTTTTTCAGCACATACTCAACAAAGATCTGCCACTTGGTCATATCGGTAGATTCTGTCTC  >  NC_000913/3846196‑3846453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑