Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,090,640	C→T	V96I (GTA→ATA)	frvX ←	peptidase M42 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,090,640	0	C	T	100.0%	92.1 / NA	28	V96I (GTA→ATA)	frvX	peptidase M42 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/14);  total (14/14)

CCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGAC  >  NC_000913/4090516‑4090768                                                                                                                             |                                                                                                                                 ccAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCggtggt                                                                                                                          <  2:584115/133‑1 (MQ=255)                  ctcATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGa                                                                                                                <  2:331132/126‑1 (MQ=255)                  ctcATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGa                                                                                                                >  1:331132/1‑126 (MQ=255)                  ctcATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTc                                                                                                         <  1:478615/133‑1 (MQ=255)                     atcaAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTccac                                                                                                      >  1:153610/1‑133 (MQ=255)                     atcaAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTccac                                                                                                      >  2:152930/1‑133 (MQ=255)                     atcaAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTccac                                                                                                      >  1:585149/1‑133 (MQ=255)                          aTGACAGCGGTTGTTGCTTTTGCTTTTCCGTTCACGCATGATGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAgcc                                                                                                 <  2:167060/133‑1 (MQ=255)                                    ttgttgCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAAtggtg                                                                                       >  2:351658/1‑133 (MQ=255)                                       ttgcttttgcttTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATGGACTGATACCAACACCCGCCAGTGGTGGTa                                                                                    >  1:181529/1‑133 (MQ=255)                                                      cGTTAACGCATGAGGCGCTACGTAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCg                                                                     <  1:166800/133‑1 (MQ=255)                                                                tGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAGCGCGGAAAACCGCTCTCGTCGa                                                           >  1:189840/1‑133 (MQ=255)                                                                tGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGa                                                           <  1:139551/133‑1 (MQ=255)                                                                   ggCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGAtgt                                                        >  2:577456/1‑133 (MQ=255)                                                                                   tCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGcc                                        <  1:149904/133‑1 (MQ=255)                                                                                     acacCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGa                                      <  2:27518/133‑1 (MQ=255)                                                                                       acCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACt                                    <  1:351658/133‑1 (MQ=255)                                                                                       acCAGGGATTTTCACT‑CCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTACACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACtt                                   >  2:42851/1‑133 (MQ=255)                                                                                        ccAGGGATTTGCACTCCCTTGTGTGTGCTTATCGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACtt                                   <  2:207709/133‑1 (MQ=255)                                                                                                 ttCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCatat                          >  1:111113/1‑133 (MQ=255)                                                                                                 ttCACTCCCTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCatat                          >  2:47118/1‑133 (MQ=255)                                                                                                       cccTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGa                    >  2:432497/1‑133 (MQ=255)                                                                                                        ccTTGTGTGTGCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGAc                   <  1:152930/133‑1 (MQ=255)                                                                                                                  gCGTATGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACt         >  2:16474/1‑133 (MQ=255)                                                                                                                       tGGTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTgg    >  2:59705/1‑133 (MQ=255)                                                                                                                       tGGTTATCCGCTGGTTGAGCATCGCCTGTTTCCACCAGCCGGCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTgg    <  2:198061/133‑1 (MQ=255)                                                                                                                         gTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGAc  <  2:111113/133‑1 (MQ=255)                                                                                                                         gTTATCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGAc  <  2:585149/133‑1 (MQ=255)                                                                                                                             |                                                                                                                                 CCAATATCAATGAACATCTCATCAAATGACAGCGGTTGTTGCTTTTGCTTTTCCGTTAACGCATGAGGCGCGACGGAACCAATCACACCAGGGATTTTCACTCCCTTGTGTGTGCGTATGGTTACCCGGTGGTTGAGCATCGACTGATTCCACCAGCCGCCAATGGTGGTAAAACGCAGAAAACCGCTCTCGTCGATGTGGGTGACCATAAAGCCGACTTCATCCATATGTCCGACAACGGCAACTTTTGGAC  >  NC_000913/4090516‑4090768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑