Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,205,209	A→C	D241E (GAT→GAG)	purD ←	phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,205,209	0	A	C	100.0%	75.7 / NA	23	D241E (GAT→GAG)	purD	phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (12/11);  total (12/11)

TGTCGATCATCAGGCCCGCGTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCATCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTCCACCATC  >  NC_000913/4205100‑4205335                                                                                                              |                                                                                                                               tGTCGATCATCAGGCCCGCGTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGa                                                                                                         <  2:477239/133‑1 (MQ=255)                  gcgTAGAGTAAACCGGTGGAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCccgc                                                                                        <  1:236789/133‑1 (MQ=255)                  gcgTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCccgc                                                                                        <  1:5156/133‑1 (MQ=255)                   cgTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCccgcc                                                                                       >  2:324286/1‑133 (MQ=255)                   cgTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTAACGGCGCGGGGGGGTAAGCGCCCATCccgcc                                                                                       >  1:199590/1‑133 (MQ=255)                        agaAAACCGGTGTAGG‑‑‑TGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTg                                                                               <  1:459788/133‑1 (MQ=255)                                         gtTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTa                                                                 <  1:324286/133‑1 (MQ=255)                                            gCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCg                                                              >  2:556533/1‑133 (MQ=255)                                                   gccgccATGCCTTTCACGGTTGGCGAGACAATCCGTTCCATTGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTAcgc                                                       <  2:267363/133‑1 (MQ=255)                                                    ccgccATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCTATCCCGCCGGTGATTGGTCCGGTATATTTATCGCCTAcgcg                                                      >  2:268007/1‑133 (MQ=255)                                                        cATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTtg                                                  <  2:547052/133‑1 (MQ=255)                                                                        ggCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAg                                  >  1:441639/1‑133 (MQ=255)                                                                                        ccATGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATg                  >  1:347440/1‑133 (MQ=255)                                                                                          aTGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCt                >  2:137736/1‑133 (MQ=255)                                                                                          aTGGTGCGCTGATGAACGCCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCt                <  1:137736/133‑1 (MQ=255)                                                                                           tGGTGCGCTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTc               >  1:61453/1‑133 (MQ=255)                                                                                                cgcTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGt          >  2:333599/1‑133 (MQ=255)                                                                                                cgcTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGt          >  1:73115/1‑133 (MQ=255)                                                                                                  cTGATGAACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTcc        <  1:550771/133‑1 (MQ=255)                                                                                                     atgaACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCt                >  1:93017/1‑122 (MQ=255)                                                                                                     atgaACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCt                <  2:93017/122‑1 (MQ=255)                                                                                                       gaACGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTCCACCAt   <  1:333599/133‑1 (MQ=255)                                                                                                        aaCGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGTTCGCCGCCCACCATc  >  1:269178/1‑133 (MQ=255)                                                                                                        aaCGTCCTCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTCCACCATc  <  2:73115/133‑1 (MQ=255)                                                                                                        aaCGCCCTCGTTTACTACCGGCGCTGGGCAGTGAGCGCCCTGCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTCCACCATc  <  2:199590/133‑1 (MQ=255)                                                                                                              |                                                                                                                               TGTCGATCATCAGGCCCGCGTAGAGAAAACCGGTGTAGGTGTTGCCTTCCGCCGCCATGCCTTTCACGGTTGGCCAGATAATACGTTCCATGGTGCGCTGATGAACGTCATCGGTTACTACCGGCGCGGGGGAGTAAGCGCCCATCCCGCCGGTGTTTGGTCCGGTATCTTTATCGCCTACGCGTTTGTGATCCTGGCTGGTAGCCATCGGCAGCACATGCTCGCCGTCCACCATC  >  NC_000913/4205100‑4205335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑