Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,262,370	G→A	E234E (GAG→GAA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,262,370	0	G	A	100.0%	130.5 / NA	38	E234E (GAG→GAA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/17);  total (21/17)

GCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCC  >  NC_000913/4262241‑4262492                                                                                                                                  |                                                                                                                           gCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGTTTACCGGAGGTATCAATTCGCTGGAAGAAGcc                                                                                                                         >  2:255686/1‑133 (MQ=255) gCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGTTTAACGGGGGTATCAATTCGCTGGAAGAAGcc                                                                                                                         >  2:251030/1‑133 (MQ=255)       ggggTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGca                                                                                                                   <  2:496415/133‑1 (MQ=255)           ttAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACAcc                                                                                                               >  1:23922/1‑133 (MQ=255)           ttAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACAcc                                                                                                               >  1:421336/1‑133 (MQ=255)                     aagaaaACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACatat                                                                                                     >  1:30049/1‑133 (MQ=255)                        aaaaCCGTTAAATCCCGCTGCTTGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATatgga                                                                                                  <  1:226215/133‑1 (MQ=255)                             cgggAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCg                                                                                             <  2:157718/130‑1 (MQ=255)                             cGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCg                                                                                             <  2:512075/133‑1 (MQ=255)                             cGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCg                                                                                             <  2:405130/133‑1 (MQ=255)                               tGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTg                                                                                           >  1:410547/1‑133 (MQ=255)                                          cgcTCGATTAACCGCTTGTGTATCTACTGAAGCGTGACTTTGCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGgcg                                                                                <  2:261426/133‑1 (MQ=255)                                            cTCGATTATCCGCGTGTGTATCACGTCAGGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGcgcg                                                                              <  2:287139/133‑1 (MQ=255)                                                      cgcgTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTACAACATATGGATGGCGGGATGGTCGGGCGCCAGGCGTATCa                                                                    >  2:161976/1‑133 (MQ=255)                                                         gtgtgtATCAACTGAAGCGTGATTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGaa                                                                 <  1:266439/133‑1 (MQ=255)                                                         gtgtgtATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGaa                                                                 >  2:497156/1‑133 (MQ=255)                                                              tATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGACGCCAGGCGTATCAGAATCCgg                                                            >  1:276392/1‑133 (MQ=255)                                                                   aCTGAAGCCTGACTTCCCCCATCTGACAATCTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTAtt                                                       <  1:161976/133‑1 (MQ=255)                                                                     tGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCt                                                     >  1:486321/1‑133 (MQ=255)                                                                      gAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTg                                                    >  2:429787/1‑133 (MQ=255)                                                                      gAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTg                                                    >  2:379004/1‑133 (MQ=255)                                                                       aaGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTgg                                                   <  1:572390/133‑1 (MQ=255)                                                                       aaGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTgg                                                   <  1:497156/133‑1 (MQ=255)                                                                        aGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTggc                                                  <  2:23922/133‑1 (MQ=255)                                                                              aCTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGt                                            >  2:395407/1‑133 (MQ=255)                                                                                 ttCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGa                                         <  1:379004/133‑1 (MQ=255)                                                                                       aTCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGga                                   <  1:380103/133‑1 (MQ=255)                                                                                                 gTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGTGGCGGTAGACCGGGAGATCTTTGGt                         <  2:421336/133‑1 (MQ=255)                                                                                                 gTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGt                         >  1:393077/1‑133 (MQ=255)                                                                                                 gTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGt                         <  2:30049/133‑1 (MQ=255)                                                                                                          cGGTGGTATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGAt                >  1:554552/1‑133 (MQ=255)                                                                                                               gtATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGa           >  2:346079/1‑133 (MQ=255)                                                                                                                tATCAAGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGAACTTTGGATCCTCGGATACCGAt          >  1:240468/1‑133 (MQ=255)                                                                                                                    aaGTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCCATGCCg      >  2:147609/1‑133 (MQ=255)                                                                                                                      gTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGAt    >  2:494344/1‑133 (MQ=255)                                                                                                                      gTCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAAATCTTTGGTTCGTTCGATACCTATGCCGAt    >  1:288727/1‑133 (MQ=255)                                                                                                                       tCGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATc   >  1:140010/1‑133 (MQ=255)                                                                                                                        cGCTGGAAGAAGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATcc  <  2:486321/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                           GCTTTCGGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTTCCGCATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAGGCCAAAGCACACCTGCAACATATGGATGGCGTGATGGTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGCCGATCC  >  NC_000913/4262241‑4262492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑