Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	423,231	G→A	Q238Q (CAG→CAA)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,231	0	G	A	100.0%	98.6 / NA	30	Q238Q (CAG→CAA)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/17);  total (13/17)

CTGTATCTCAATCCGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCG  >  NC_000913/423106‑423350                                                                                                                              |                                                                                                                        cTGTATCTCAATCCGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGaa                                                                                                                  >  2:384360/1‑133 (MQ=255)       ctcAATCCGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGc                                                                                                            <  2:367521/133‑1 (MQ=255)            tCCGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGAGGCTGGTg                                                                                                       >  1:225362/1‑133 (MQ=255)              cGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCt                                                                                                     >  2:524274/1‑133 (MQ=255)                     tAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGc                                                                                              <  1:384360/133‑1 (MQ=255)                      aaaGCTCCCAGCGTAGATAAATACTATAGCGACGATTATCGGCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCg                                                                                             <  2:225362/133‑1 (MQ=255)                      aaaGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCg                                                                                             >  2:475308/1‑133 (MQ=255)                           tCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGttt                                                                                        <  2:422774/133‑1 (MQ=255)                             ccAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGGTGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTaa                                                                                      >  1:65414/1‑133 (MQ=255)                             ccAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTaa                                                                                      >  1:226417/1‑133 (MQ=255)                               aGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGTTGCTGGGCGGGGGGTTTAAcc                                                                                    >  2:426232/1‑133 (MQ=255)                                gcatACATAAATACGACACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACca                                                                                   <  2:442199/130‑1 (MQ=255)                                         aaTACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGa                                                                          <  2:497401/133‑1 (MQ=255)                                                tACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAATCACAGTGGCTATTCCCAt                                                                   >  1:260250/1‑133 (MQ=255)                                                     aGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTg                                                              >  2:560666/1‑133 (MQ=255)                                                          tATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTg                                                         <  2:77666/133‑1 (MQ=255)                                                          tATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTg                                                         >  1:39282/1‑133 (MQ=255)                                                           aTCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGa                                                        <  2:27508/133‑1 (MQ=255)                                                            tCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGAc                                                       <  1:484785/133‑1 (MQ=255)                                                               ccATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAgg                                                    <  1:437573/133‑1 (MQ=255)                                                                        tCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGt                                           <  1:524274/133‑1 (MQ=255)                                                                           gCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGc                                        <  1:98217/133‑1 (MQ=255)                                                                           gCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGc                                        <  2:226417/133‑1 (MQ=255)                                                                            cAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCa                                       <  1:578638/133‑1 (MQ=255)                                                                                ttGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACggg                                   <  1:560666/133‑1 (MQ=255)                                                                                ttGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACggg                                   <  1:355044/133‑1 (MQ=255)                                                                                 tGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGggt                                  >  1:541824/1‑133 (MQ=255)                                                                                             ggCGTTGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTcac                      >  2:589832/1‑133 (MQ=255)                                                                                                 ttGCTGCGTTTGCGACACAATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAAcc                  >  1:47991/1‑133 (MQ=255)                                                                                                                 acaATACGCAGCAACTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGgcg  <  2:39282/133‑1 (MQ=255)                                                                                                                              |                                                                                                                        CTGTATCTCAATCCGGTGTTTAAAGCTCCCAGCGTACATAAATACGATACCGAGGATTATCGCCATGTCGATCCGCAGTTTGGCGGTGATGGGGCGTTGCTGCGTTTGCGACACAATACGCAGCAGCTGGGAATGCGGCTGGTGCTGGACGGCGTGTTTAACCACAGTGGCGATTCCCATGCCTGGTTTGACAGGCATAATCGTGGCACGGGTGGTGCTTGTCACAACCCCGAATCGCCCTGGCG  >  NC_000913/423106‑423350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑