Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,300,748	A→G	S95P (TCC→CCC)	mdtP ←	putative multidrug efflux pump outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,300,748	0	A	G	100.0%	105.7 / NA	31	S95P (TCC→CCC)	mdtP	putative multidrug efflux pump outer membrane channel
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/15);  total (16/15)

TGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGG  >  NC_000913/4300629‑4300873                                                                                                                        |                                                                                                                              tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTttct                                                                                                                  >  1:368185/1‑133 (MQ=255) tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTttct                                                                                                                  >  1:329772/1‑133 (MQ=255) tGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGGAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTttct                                                                                                                  >  2:74383/1‑133 (MQ=255)           tCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGtt                                                                                                        >  2:72119/1‑133 (MQ=255)                    tAAGGGCTTAAAAAGCCGTTCGCCCAGACACGTTGGCGGTTAAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCgg                                                                                               <  2:220461/133‑1 (MQ=255)                     aaGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGc                                                                                              <  1:98844/133‑1 (MQ=255)                         gCTTAAAAAGTCGTTCGCCGAGACTCGTTGGCGGTTGAGCATCCCTAACTCTGCGACCTGTAATTGTGGACCGGCATCTAGCAAATCGGCCTGGGGCTGCCCTTTTTCGTCCCGCAGTTTCGCTTCGGCGAgg                                                                                          <  2:380326/133‑1 (MQ=255)                         gCTTAAAAAGCCGTTTGCTGAGGCACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAgg                                                                                          <  1:239768/133‑1 (MQ=255)                         gCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAgg                                                                                          <  1:489912/133‑1 (MQ=255)                         gCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAgg                                                                                          <  1:3145/133‑1 (MQ=255)                         gCTTAAAAAGCCGTTAGCCGAGACTCGTCGGCAGTTGAGCACCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAgg                                                                                          <  2:170919/133‑1 (MQ=255)                            tAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTATCTTCCCCCAGTTTAGCTTCGGCGAGGgtg                                                                                       >  1:235272/1‑133 (MQ=255)                              aaaaGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGgtgtg                                                                                     >  1:47299/1‑133 (MQ=255)                                            gagaCACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCg                                                                       <  2:381233/133‑1 (MQ=255)                                            gagaCACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCg                                                                       <  1:606708/133‑1 (MQ=255)                                                cacGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCAGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCg                                                                   >  2:113277/1‑133 (MQ=255)                                                                      tAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGc                                             <  2:14328/133‑1 (MQ=255)                                                                          gCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGt                                         >  2:111964/1‑133 (MQ=255)                                                                            tGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCa                                       <  2:348254/133‑1 (MQ=255)                                                                             gCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATAAGCACATCCAGCTACGGGTCAt                                      >  1:291868/1‑133 (MQ=255)                                                                              cGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCAtt                                     >  2:138555/1‑133 (MQ=255)                                                                              cGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCAtt                                     >  2:317507/1‑133 (MQ=255)                                                                                 ccTGTAATTGTGAACCGGCATCTAACAAATCAGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAg                                  <  1:113277/133‑1 (MQ=255)                                                                                 ccTGTAATTGTGAACCGGAATCTAACAAATAGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAg                                  <  1:203137/133‑1 (MQ=255)                                                                                   tGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAgtt                                <  2:368185/133‑1 (MQ=255)                                                                                        ttGTGAACCGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTc                           <  1:74383/133‑1 (MQ=255)                                                                                         tgtgAACCGGTATCTTACAAATCGGCCTGGGGATGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTcc                          <  2:220554/133‑1 (MQ=255)                                                                                               ccGGCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCTGCTGCGGCTCATTGTGTTGTTCCCACCACt                    >  1:220554/1‑133 (MQ=255)                                                                                                  gCATCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTgcg                 >  2:624414/1‑133 (MQ=255)                                                                                                     tCTAACAAATCGGCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCt              >  1:382657/1‑133 (MQ=255)                                                                                                                 gCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCgg  >  1:361132/1‑133 (MQ=255)                                                                                                                 gCCTGGGGCTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCgg  >  1:344879/1‑133 (MQ=255)                                                                                                                        |                                                                                                                              TGCTGGCGCATCCATTGAATAAGGGCTTAAAAAGCCGTTCGCCGAGACACGTTGGCGGTTGAGCATCCCTAACGCTGCGACCTGTAATTGTGAACCGGCATCTAACAAATCGGCCTGGGACTGCGCTTTTTCTTCCCGCAGTTTCGCTTCGGCGAGGGTGTGTGAACCACTTAGCGTCCGTTGGATCAGCGCATCCAGCTGCGGGTCATTGAGTTGTTTCCACCACTGCGCCTGCGGCCAGCCGG  >  NC_000913/4300629‑4300873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑