Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,329,752	A→G	E131E (GAA→GAG)	yjcZ →	uncharacterized protein YjcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,329,752	0	A	G	100.0%	86.6 / NA	25	E131E (GAA→GAG)	yjcZ	uncharacterized protein YjcZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/9);  total (16/9)

ACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGG  >  NC_000913/4329624‑4329879                                                                                                                                 |                                                                                                                                aCGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAgg                                                                                                                             >  2:337612/1‑133 (MQ=255)              agcaTCTGGCGTTGTTGAAAAAAAGGCTGCTCCGACAGCACTCTGCTTTCCCGGAGCGGCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACg                                                                                                                <  2:54336/133‑1 (MQ=255)              agcaTCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACg                                                                                                                <  2:499704/133‑1 (MQ=255)                     ggCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTa                                                                                                         <  1:337612/133‑1 (MQ=255)                            ctgcAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTgc                                                                                                  >  1:475684/1‑133 (MQ=255)                             tgcAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTgca                                                                                                 <  2:475684/133‑1 (MQ=255)                               cAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCATTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTgcagg                                                                                               <  1:576254/133‑1 (MQ=255)                                         aTCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCa                                                                                     >  2:395096/1‑133 (MQ=255)                                                ccAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAAGCCAAATGATGCAGGCAGGTGACCAgagcgag                                                                              >  2:296208/1‑133 (MQ=255)                                                               cccGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGCAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGc                                                               >  2:309121/1‑133 (MQ=255)                                                                   gAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGc                                                           >  1:448828/1‑133 (MQ=255)                                                                   gAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGc                                                           >  2:114581/1‑133 (MQ=255)                                                                   gAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGc                                                           >  2:463615/1‑133 (MQ=255)                                                                        cccccAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCt                                                      <  1:309121/133‑1 (MQ=255)                                                                         ccccAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTTATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTg                                                     <  1:492648/133‑1 (MQ=255)                                                                           ccAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTggg                                                   <  2:448828/133‑1 (MQ=255)                                                                                        gccTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTATTACCCGCTGGCGTGCTGGGGCGTATAACGCCt                                      >  2:336484/1‑133 (MQ=255)                                                                                                tGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTg                              >  2:38651/1‑133 (MQ=255)                                                                                                      gTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTggg                        >  2:494067/1‑133 (MQ=255)                                                                                                      gTTTTCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTggg                        >  1:47457/1‑133 (MQ=255)                                                                                                        tttCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGgcg                      >  1:486813/1‑133 (MQ=255)                                                                                                        tttCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGgcg                      >  1:122679/1‑133 (MQ=255)                                                                                                         ttCAGCCCGTTGTCGCTGGAAGGGGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGcc                            <  1:222379/126‑1 (MQ=255)                                                                                                         ttCAGCCCGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGTGTATAACGCCTGGTCGTTGcc                            >  2:222379/1‑126 (MQ=255)                                                                                                              cccGTTGTCGCTGGAAGGAGAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGttt                >  2:10801/1‑133 (MQ=255)                                                                                                                            aaggagAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTAt             <  2:243541/122‑1 (MQ=255)                                                                                                                            aaggagAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTAt             >  1:243541/1‑122 (MQ=255)                                                                                                                            aaggagAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTggagg  >  1:544486/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                ACGCAGATCGCGCAGCATCTGGCGCTGCTGCAAAAAATGGATCACCGCCAGCACTCTGCTTTCCCGGAGCTCCCCCAGCAAATTGCCGCCTTGTATGAGTGGTTTTCAGCCCGTTGTCGCTGGAAGGAAAAGGCGTTAACGCAACGAGGCCTACTGGTGCAGGCAGGTGATCAGAGCGAGCAAATTTTTACCCGCTGGCGTGCTGGGGCGTATAACGCCTGGTCGTTGCCTGGGCGCTGTTTTATCGTTCTGGAGG  >  NC_000913/4329624‑4329879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑