Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,359,871	G→A	A54V (GCG→GTG)	cadB ←	lysine:cadaverine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,359,871	0	G	A	100.0%	199.3 / NA	59	A54V (GCG→GTG)	cadB	lysine:cadaverine antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/39);  total (20/39)

TACCAATCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACGCCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTACCGGCAACAACACCGGTACA  >  NC_000913/4359743‑4359998                                                                                                                                 |                                                                                                                                tACCAATCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAg                                                                                                                             <  1:579851/133‑1 (MQ=255)  aCCAATCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGc                                                                                                                            <  1:588139/133‑1 (MQ=255)    cAATCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGa                                                                                                                          >  1:14766/1‑133 (MQ=255)      aTCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACa                                                                                                                        <  2:116214/133‑1 (MQ=255)         cAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTg                                                                                                                     >  1:212503/1‑133 (MQ=255)         cAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTg                                                                                                                     >  2:532455/1‑133 (MQ=255)          aGTTAGCATGGTAATTAATAACGCCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGc                                                                                                                    <  1:256863/133‑1 (MQ=255)            ttAGCATGGTAATAAAGATCACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCAc                                                                                                                  <  1:268210/133‑1 (MQ=255)            ttAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCAc                                                                                                                  <  2:469481/133‑1 (MQ=255)             tAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCAcc                                                                                                                 <  1:24831/133‑1 (MQ=255)              aGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCa                                                                                                                <  1:569375/133‑1 (MQ=255)                 aTGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCAcc                                                                                                                 <  2:4066/129‑1 (MQ=255)                 aTGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCAcc                                                                                                                 >  1:4066/1‑129 (MQ=255)                  tGGTAATAATGAACAACTGTCTGTAAACGAAACGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCaataa                                                                                                            <  2:290199/133‑1 (MQ=255)                   ggTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCaataat                                                                                                           <  1:554499/133‑1 (MQ=255)                    gTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAataata                                                                                                          >  1:309564/1‑133 (MQ=255)                      aataaAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACTAGCGACATTGCACCAATAATaga                                                                                                        >  2:274157/1‑133 (MQ=255)                       ataaAGAACACCTGTCTGCAAACCAAATGCTGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATagag                                                                                                       <  1:171571/133‑1 (MQ=255)                        taaAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAgaga                                                                                                      >  1:442291/1‑133 (MQ=255)                         aaaGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGAt                                                                                                     <  1:546540/133‑1 (MQ=255)                             aaCACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATc                                                                                                 <  1:443181/133‑1 (MQ=255)                              acacCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATcc                                                                                                >  1:323870/1‑133 (MQ=255)                               cacCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCa                                                                                               <  2:369535/133‑1 (MQ=255)                               cacCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTCCACTAATAATAGATATAATCCa                                                                                               >  1:262690/1‑133 (MQ=255)                               cacCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATAAACATACACGAGCCACAATGCACCAATAATAGAGATAATCCa                                                                                               >  2:171571/1‑133 (MQ=255)                                  cTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAAcc                                                                                            >  1:501958/1‑133 (MQ=255)                                   tGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAAccc                                                                                           <  1:532455/133‑1 (MQ=255)                                    gTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACGTTGCACCAATAATACAGATAAGCCAAcccc                                                                                          >  1:197324/1‑133 (MQ=255)                                    gTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAAcccc                                                                                          >  1:434214/1‑133 (MQ=255)                                    gTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAAcccc                                                                                          >  1:380067/1‑133 (MQ=255)                                     tCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCa                                                                                         <  2:65047/133‑1 (MQ=255)                                     tCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCa                                                                                         <  2:434214/133‑1 (MQ=255)                                     tCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCa                                                                                         <  2:157408/133‑1 (MQ=255)                                     tCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCa                                                                                         >  1:78883/1‑133 (MQ=255)                                     tCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACAATGCACCAATAAAAGTGATAATTCAACCCCa                                                                                         >  1:276484/1‑133 (MQ=255)                                      cTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAg                                                                                        >  2:123192/1‑133 (MQ=255)                                        gAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGAt                                                                                      <  2:212503/133‑1 (MQ=255)                                         aaaaCCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATa                                                                                     <  2:512834/133‑1 (MQ=255)                                                   gCAGGGGAAATTTCTCCGGCATGAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACTAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATaccac                                                                           <  1:274157/133‑1 (MQ=255)                                                              ttCTCCGGCATAAGCCATGGGGCCACCTTGCTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCt                                                                <  1:216032/133‑1 (MQ=255)                                                              ttCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCt                                                                <  2:14766/133‑1 (MQ=255)                                                              ttCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCt                                                                <  2:442291/133‑1 (MQ=255)                                                                  ccGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGt                                                            <  2:561678/133‑1 (MQ=255)                                                                     gCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCg                                                         <  2:309564/133‑1 (MQ=255)                                                                       aTAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCa                                                       <  1:577588/133‑1 (MQ=255)                                                                         aaGCAATTTGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAgg                                                     <  1:270646/133‑1 (MQ=255)                                                                          aGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGt                                                    <  1:433021/133‑1 (MQ=255)                                                                                   gCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGtaa                                                  <  1:137615/126‑1 (MQ=255)                                                                                   gCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGtaa                                                  >  2:137615/1‑126 (MQ=255)                                                                                    ccACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCaa                                          <  2:501958/133‑1 (MQ=255)                                                                                       ccTTGTTGCGGGTTTCTTGTTGCCTGCCTGGCGTATACATATACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATAc                                       <  2:276484/133‑1 (MQ=255)                                                                                           gttgCGGGTTTTTAGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCt                                   <  2:573568/133‑1 (MQ=255)                                                                                            ttgCGGGTTTTTTGTAGCCAGTCGAGCACATATATACACCACCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTc                                  <  2:159438/133‑1 (MQ=255)                                                                                                 ggTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCcat                             >  1:55981/1‑133 (MQ=255)                                                                                                  gTTTTTTGTTGCCAGTCGGGCATATACATACACCAGCGACATTGCACCAACAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCcatc                            <  2:380067/133‑1 (MQ=255)                                                                                                          ttgCCAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTAcc                    <  2:78883/133‑1 (MQ=255)                                                                                                             ccAGTCGGGCATATACATACACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTACCGGc                 >  2:328358/1‑133 (MQ=255)                                                                                                                gTCGGGCATAGACATACACCAGCGGCATTGCATCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTACCGGcaac              <  2:262690/133‑1 (MQ=255)                                                                                                                            catacACCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTACCGGCAACAACACCGGTACa  <  1:123192/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                TACCAATCCAGTTAGCATGGTAATAAAGAACACCTGTCTGAAAACCAAATGCAGGGGAAATTTCTCCGGCATAAGCAATTGGGCCACCTTGTTGCGGGTTTTTTGTTGCCAGTCGGGCATATACATACGCCAGCGACATTGCACCAATAATAGAGATAATCCAACCCCAGATAGCAATACCACCGATACTTGCTAGGTTCGCAGGTAATAATGCAATACCGCTCCCCATCATATTACCGGCAACAACACCGGTACA  >  NC_000913/4359743‑4359998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑