| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,362,343 | A→G | intergenic (‑409/+208) | cadC ← / ← pheU | DNA‑binding transcriptional activator CadC/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,362,343 | 0 | A | G | 97.2% | 120.7 / NA | 36 | intergenic (‑409/+208) | cadC/pheU | DNA‑binding transcriptional activator CadC/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (17/18); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAA > NC_000913/4362218‑4362470 | tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCCGCAGCATc > 1:229335/1‑133 (MQ=255)tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATc > 2:461411/1‑133 (MQ=255)tGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGATGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATc > 2:86320/1‑133 (MQ=255) gCATGAGCCAGCGAATTGCTACATTTCTCTATGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCa < 1:161912/133‑1 (MQ=255) aGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACgggg > 1:467065/1‑133 (MQ=255) aaGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCg < 2:69015/133‑1 (MQ=255) cGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCAc < 2:336160/133‑1 (MQ=255) tGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGGCGTCATTTAACGATAATGAGCATCACGGGGTCGCACGActt > 2:306074/1‑131 (MQ=255) tttCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGt < 1:463582/133‑1 (MQ=255) ctTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGtt > 1:346351/1‑133 (MQ=255) ttGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAg < 2:467065/133‑1 (MQ=255) tctcATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCt > 1:345533/1‑133 (MQ=255) cAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTc > 1:74614/1‑133 (MQ=255) gAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGTCATTTCAGGTCAGTGAGTGCTATAGCTCAg > 1:192598/1‑133 (MQ=255) tctcATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACa > 1:495436/1‑133 (MQ=255) aGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTc < 2:461352/133‑1 (MQ=255) aGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTc < 1:461411/133‑1 (MQ=255) gTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTcc < 2:345533/133‑1 (MQ=255) cGTTCAGACCGGTGTAGTGCTTTATGGATGAGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAAt < 1:243070/133‑1 (MQ=255) gtgCTTTATGGATGCTTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCaa < 1:182794/133‑1 (MQ=255) tATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTATCCCGAAATGGAATATGCCCGAAAATTGCt > 1:162085/1‑133 (MQ=255) gCGTTACGCTACTGACGACGTCATTTGAAGTTCAGCAGCATAACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGAtg < 2:192598/133‑1 (MQ=255) ttACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTg > 2:481721/1‑127 (MQ=255) ttACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTg < 1:481721/127‑1 (MQ=255) tACGCTCCTGCTGACCTCATTTGATGTTAAGCGGCATCACGTGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTggg < 2:162085/133‑1 (MQ=255) tACGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTggg < 1:511090/133‑1 (MQ=255) cGCTCCTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCCCAAGTTGCTGGTGATGTGGGga > 1:274734/1‑133 (MQ=255) ccTGATGACGCCATTTGACGTTCAACAGCATCTCGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAAt < 2:158567/133‑1 (MQ=255) cTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATc > 2:572812/1‑133 (MQ=255) cTGATGACGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGACCGCAAAGTGCGGGGGATGTGAGGAGAATc > 1:158567/1‑133 (MQ=255) gatgaCGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGCGTATGCCCGCAAATTGCTGGTGATGTGGGga < 1:553872/126‑1 (MQ=255) gatgaCGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGCGTATGCCCGCAAATTGCTGGTGATGTGGGga > 2:553872/1‑126 (MQ=255) atgaCGTCATTTGACTTTCAGCAGTATCACGGTGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTgg < 2:207229/133‑1 (MQ=255) gaCGTCATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGtt < 2:495436/133‑1 (MQ=255) cATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGtt > 1:134338/1‑133 (MQ=255) cATTTGACGTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATACCCGCAAACTGCTGGTGATGTGGGGGGAATCTGGTTGAGtt > 2:253284/1‑133 (MQ=255) ttGACGTTCAGCAGCATCTCGGAGCGGCACTACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCgg < 1:306074/133‑1 (MQ=255) gTTCAGCAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGaa < 2:229335/133‑1 (MQ=255) | TGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAGTCGCCGTTCAGACCGGTGTTGTGCTTTATGGATGCGTTACGCTCCTGATGACGTCATTTGACGTTCAACAGCATCACGGGGCCGCACGACATTTCACGTCAGTTAGTGCTATAGCTCAGGAACAAATTTTCCCGAATTGGGATATGCCCGCAAATTGCTGGTGATGTGGGGAGAATCTGGTTGAGTTCGGTAGAA > NC_000913/4362218‑4362470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |