Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,181	T→C	N278S (AAC→AGC)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,181	0	T	C	100.0%	164.7 / NA	47	N278S (AAC→AGC)	tsx	nucleoside‑specific channel‑forming protein Tsx
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/26);  total (21/26)

CGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATA  >  NC_000913/431056‑431309                                                                                                                              |                                                                                                                                 cgcATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAgtt                                                                                                                           >  1:25421/1‑133 (MQ=255)    aTCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAgttgcc                                                                                                                        <  1:561190/133‑1 (MQ=255)           aTGAACAAAGGACACTTTGTTAACAAGCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGttgccgttgccg                                                                                                                 <  2:267683/133‑1 (MQ=255)           aTGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGttgccgttgccg                                                                                                                 <  1:412561/133‑1 (MQ=255)                cAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAagtt                                                                                                            <  1:381628/133‑1 (MQ=255)                  aaGCACACGTTGTTATCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAagttca                                                                                                          <  1:497405/133‑1 (MQ=255)                        acGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTg                                                                                                    <  2:25421/133‑1 (MQ=255)                        acGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGAAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTg                                                                                                    >  2:282049/1‑133 (MQ=255)                           ttgttAACAACCAGAAATGCCGGGCATAAATCCCGGCATTTGCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAt                                                                                                 <  2:224202/133‑1 (MQ=255)                             gttAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAACTTGCCGTAGCCGAAGTTCAGTTCTGCAtcg                                                                                               >  1:203925/1‑133 (MQ=255)                                aaCAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCAtcgtcg                                                                                            <  2:90454/133‑1 (MQ=255)                                    aTCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGATGCCGAAATTCAGTTCTGCATCGTCGTTcc                                                                                        >  1:280691/1‑133 (MQ=255)                                    aTCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCAGAAGTTGCGGTTGCCGAAGTTCAGTGCTGCCTCGTCGTTcc                                                                                        >  2:171845/1‑133 (MQ=255)                                     tCAGAAATTCCGGGAATAAATCCCGGCATTATCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCa                                                                                       <  1:255739/133‑1 (MQ=255)                                     tCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCa                                                                                       <  1:82746/133‑1 (MQ=255)                                          aaTGCCGGGATTAAACCCCGGCATTTTCGTAATCAGAAGTTTTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGac                                                                                  <  2:283672/133‑1 (MQ=255)                                             gCCGGGAATAAATCCTGGTATTATCATAATAAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGaccac                                                                               <  1:189400/133‑1 (MQ=255)                                               cGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTAAGTTCTGAATCTTCTTTCCACGGACCACcg                                                                             >  1:204553/1‑133 (MQ=255)                                                        aTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAg                                                                    >  2:78735/1‑133 (MQ=255)                                                           ccGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa                                                                 <  1:66958/133‑1 (MQ=255)                                                           ccGGCATTATCATAAACATAAGTTGTAACCCACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTaa                                                                 <  1:205531/133‑1 (MQ=255)                                                             ggCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACg                                                               >  1:27090/1‑133 (MQ=255)                                                             ggCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACg                                                               >  2:312473/1‑133 (MQ=255)                                                              gCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGa                                                              >  1:562419/1‑133 (MQ=255)                                                                aTTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGc                                                            <  1:78735/133‑1 (MQ=255)                                                                  tttCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTa                                                          <  1:577094/133‑1 (MQ=255)                                                                  tttCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTa                                                          <  1:95619/133‑1 (MQ=255)                                                                    tCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCTACTGACCCCCGTCGTGACAGTAACGAGCTACg                                                        >  2:205531/1‑133 (MQ=255)                                                                     cATAATCAGTAGTTGTACCCTACGACCAGGTACCCGCCCCATCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGa                                                       <  2:306344/133‑1 (MQ=255)                                                                                           ctacCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTa                                 >  2:481665/1‑133 (MQ=255)                                                                                           ctacCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTa                                 >  1:331318/1‑133 (MQ=255)                                                                                            tacCAGGTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAg                                >  1:596247/1‑133 (MQ=255)                                                                                              ccAGGTACCCACCCCAGCTGGTGGAGCGAATGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGtt                              <  1:228734/133‑1 (MQ=255)                                                                                              ccAGGTAACCCCCCCTGCCGGTAGGGCGCACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGtt                              <  2:203925/133‑1 (MQ=255)                                                                                                 ggTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCag                           <  1:403644/133‑1 (MQ=255)                                                                                                  gTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCaga                          >  2:375598/1‑133 (MQ=255)                                                                                                  gTAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGAAACGAGCTACGAGAGAGTAGTGCCAGTGATCCTAGTTCaga                          >  2:228734/1‑133 (MQ=255)                                                                                                   tAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCagag                         >  2:13100/1‑133 (MQ=255)                                                                                                   tAACCACCCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCagag                         >  2:193/1‑133 (MQ=255)                                                                                                     accaccCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGcc                       >  1:320095/1‑133 (MQ=255)                                                                                                      ccaccCCAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCa                      >  2:73951/1‑133 (MQ=255)                                                                                                          cccAGCCGGTAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAat                  <  2:596247/133‑1 (MQ=255)                                                                                                                  gTAGAGCGAACGCTGAAGTTGCCGTTGCCGGAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTgg          >  2:384348/1‑133 (MQ=255)                                                                                                                   tAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTTCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGa         <  2:26771/133‑1 (MQ=255)                                                                                                                   tAGAGCGAACGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGa         <  1:378377/133‑1 (MQ=255)                                                                                                                       gCGAACGCTGAAGTTGCCGTTGCCCAAGTTCAATTCTGCATAGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCg     <  2:269996/133‑1 (MQ=255)                                                                                                                          aaCGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATa  <  2:331318/133‑1 (MQ=255)                                                                                                                          aaCGCTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATa  <  1:375598/133‑1 (MQ=255)                                                                                                                              |                                                                                                                                 CGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATA  >  NC_000913/431056‑431309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑