Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,474,725	C→T	intergenic (+8/‑137)	bdcR → / → tabA	putative transcriptional regulator BdcR/DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,474,725	0	C	T	100.0%	168.9 / NA	57	intergenic (+8/‑137)	bdcR/tabA	putative transcriptional regulator BdcR/DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/28);  total (29/28)

AGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGA  >  NC_000913/4474604‑4474852                                                                                                                          |                                                                                                                                aGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTAATCTTGAGGGGGGATGCTGGCTTTGACCCGaaa                                                                                                                      >  2:262802/1‑133 (MQ=255)        tGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACGATTCTTTAGGGGGGATACGGGCTTTTACCCGAAAGGCGGGa                                                                                                               >  2:275743/1‑133 (MQ=255)        tGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGa                                                                                                               >  1:101219/1‑133 (MQ=255)             tCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTTTTGTGGAGGGATGCGGGCTTTTACCCGAAAGGCGGGGAAgag                                                                                                          >  2:374318/1‑131 (MQ=255)             tCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAgcg                                                                                                          >  1:342288/1‑133 (MQ=255)                 ggCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTg                                                                                                      >  1:597716/1‑133 (MQ=255)                     ttCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGa                                                                                                  <  2:3552/133‑1 (MQ=255)                      tCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 <  2:597716/133‑1 (MQ=255)                      tCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 <  2:32227/133‑1 (MQ=255)                      tCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 >  2:31502/1‑133 (MQ=255)                      tCTGCGAAGGCACGAAAGGGGCCCTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 <  1:275743/133‑1 (MQ=255)                            aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 >  1:341592/1‑127 (MQ=255)                            aaGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGAt                                                                                                 <  2:341592/127‑1 (MQ=255)                             aGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTtgt                                                                                          <  2:472431/133‑1 (MQ=255)                                cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGAt                                                                                       <  2:602415/133‑1 (MQ=255)                                cACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGAt                                                                                       <  1:374318/133‑1 (MQ=255)                                  cGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATaa                                                                                     <  2:101219/133‑1 (MQ=255)                                              tctATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCa                                                                         <  2:389285/133‑1 (MQ=255)                                                    gAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGtt                                                                   <  1:14895/133‑1 (MQ=255)                                                       caacTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAg                                                                >  2:593670/1‑133 (MQ=255)                                                         acTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGc                                                              <  1:544702/133‑1 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGGAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:50973/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGGGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:602179/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:111698/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:82330/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:367985/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  2:521897/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  1:139906/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGGTAAGGCt                                                             >  2:316891/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAAATCACGCGTTAAGGCt                                                             >  2:390696/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGGTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  2:84001/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCTTTGACCCGAAAGGCGGGGAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  2:134461/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  2:41673/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAAATATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCt                                                             >  2:592093/1‑133 (MQ=255)                                                          ctctGTGCAACAGCTGCAATGGCGGGGGAAACGATAAAAACTATTCTTGCGGAGCGATGCGGGCTTTGTCCCGAAAGGCGGGAAAGAGCCTCCCGTGAAGATGGGATACAACAAAAATTCACTCGGTAAGGcg                                                             >  2:257616/1‑132 (MQ=255)                                                            ctGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAg                                                           >  2:480097/1‑133 (MQ=255)                                                               tgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATCAGACAATAATTGACCCGTTAAAGCTAGCgg                                                        >  1:245551/1‑133 (MQ=255)                                                               tgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCgg                                                        >  2:101824/1‑133 (MQ=255)                                                               tgCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGGGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCgg                                                        >  2:436556/1‑133 (MQ=255)                                                                 cAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGaa                                                      <  2:342288/133‑1 (MQ=255)                                                                     aGCTGCAATGGCGGGGGGAGGGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGa                                                  >  2:419355/1‑133 (MQ=255)                                                                                   gggAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGtat                                    <  2:136823/133‑1 (MQ=255)                                                                                         cGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGAt                              >  1:35441/1‑133 (MQ=255)                                                                                         cGATAAAAACTATTCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGAt                              <  1:436556/133‑1 (MQ=255)                                                                                                     ttCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg                  <  1:519425/133‑1 (MQ=255)                                                                                                     ttCTTGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg                  <  2:79611/133‑1 (MQ=255)                                                                                                     ttCTTGAGCAGTGATCCGCGCTATGACCCGGAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACg                  <  1:197433/133‑1 (MQ=255)                                                                                                         tGAGGAGTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgttgt              <  2:413316/133‑1 (MQ=255)                                                                                                          gaggagTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACgttgtt             >  1:325625/1‑133 (MQ=255)                                                                                                           aggagTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGttt            >  2:41485/1‑133 (MQ=255)                                                                                                           aggagTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGttt            <  1:41485/133‑1 (MQ=255)                                                                                                              agTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaa         <  1:593670/133‑1 (MQ=255)                                                                                                               gTGATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaat        >  2:539439/1‑133 (MQ=255)                                                                                                                tGATGCGGGCTTTGAACCGAAAGGCGGGTAAGGGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaatt       <  2:245551/133‑1 (MQ=255)                                                                                                                 gATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaatta      <  1:41673/133‑1 (MQ=255)                                                                                                                 gATGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaatta      <  2:139906/133‑1 (MQ=255)                                                                                                                  aTGCGGGCTTTGAACCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTttaattaa     >  1:461429/1‑133 (MQ=255)                                                                                                                   tGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAg    <  1:390696/133‑1 (MQ=255)                                                                                                                   tGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAg    <  2:50973/133‑1 (MQ=255)                                                                                                                   tGCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAg    <  1:521897/133‑1 (MQ=255)                                                                                                                    gCGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAgg   >  2:104435/1‑133 (MQ=255)                                                                                                                     cGGGCTTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGa  <  1:31502/133‑1 (MQ=255)                                                                                                                          |                                                                                                                                AGTACCGTGATGTCTGGGCTTTCTGCGAAGGCACGAGAGGGGCACTCTATAGAACAACTCTGTGCAACAGCTGCAATGGCGGGGGAAGCGATAAAAACTATTCTTGAGGAGTGATGCGGGCCTTGACCCGAAAGGCGGGAAAGCGCCTGCCGATAAGTTGTGATAAGACAATAATTCACGCGTTAAGGCTAGCGGAATTGATTATCTTTTCGTATAACGATAGAAATGAAACGTTGTTTTAATTAAGGA  >  NC_000913/4474604‑4474852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑