Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,525,117	A→G	N229N (AAT→AAC)	yjhI ←	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,525,117	0	A	G	100.0%	159.7 / NA	44	N229N (AAT→AAC)	yjhI	KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

TAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATC  >  NC_000913/4524989‑4525244                                                                                                                                 |                                                                                                                                taatGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTc                                                                                                                             <  1:537628/133‑1 (MQ=255) taatGCCGCTGAATTGTTTCATTATCTCAGTTTCCTTTTCGCAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTc                                                                                                                             <  2:274347/133‑1 (MQ=255)      ccGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGaa                                                                                                                        >  1:20090/1‑133 (MQ=255)      ccGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGaa                                                                                                                        >  1:491713/1‑133 (MQ=255)      ccGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGaa                                                                                                                        >  1:371993/1‑133 (MQ=255)         cTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATaa                                                                                                                     >  1:406083/1‑133 (MQ=255)                        aCCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATGTTGTTTCATGAGTAAACTACCCGATCGCCa                                                                                                      >  1:181659/1‑133 (MQ=255)                        aCCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCa                                                                                                      <  2:406083/133‑1 (MQ=255)                           tCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGaa                                                                                                   >  2:123935/1‑133 (MQ=255)                                  ccTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTgaga                                                                                            <  1:123935/133‑1 (MQ=255)                                  ccTTTTCGTAAGTGCTCATTTTCGCTTCCGTACCCCAGCCCCTATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTgaga                                                                                            <  2:181659/133‑1 (MQ=255)                                    ttttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCTCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGATTAAACTACCGGATCGCCAGAAAGTGAGAtt                                                                                          >  2:92055/1‑133 (MQ=255)                                      ttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGc                                                                                        <  2:491713/133‑1 (MQ=255)                                      ttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGc                                                                                        <  2:20090/133‑1 (MQ=255)                                      ttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGc                                                                                        <  1:65198/133‑1 (MQ=255)                                      ttCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGc                                                                                        <  1:597504/133‑1 (MQ=255)                                              tGCTCATTTTAGTTTTTGTATCCCTGCCTATACGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTaa                                                                                <  2:232634/133‑1 (MQ=255)                                                cTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAAct                                                                              <  2:400514/133‑1 (MQ=255)                                                cTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAAct                                                                              <  1:148125/133‑1 (MQ=255)                                                cTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAAct                                                                              <  1:566319/133‑1 (MQ=255)                                                cTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAAct                                                                              >  1:400514/1‑133 (MQ=255)                                                cTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAAct                                                                              <  1:479782/133‑1 (MQ=255)                                                       tCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTtat                                                                       <  1:57738/133‑1 (MQ=255)                                                                       gCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCgg                                                       <  2:370911/133‑1 (MQ=255)                                                                        cccATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCgg                                                       >  1:595479/1‑132 (MQ=255)                                                                        cccATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCgg                                                       <  2:595479/132‑1 (MQ=255)                                                                         ccATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCa                                                     <  2:371993/133‑1 (MQ=255)                                                                                      gCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATAGAATACCGGCATAGTTAAACATAc                                        >  2:252448/1‑133 (MQ=255)                                                                                       cTCAGCGCTTTTCCGAAGGAATTCCAGATATCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATAcc                                       <  1:280710/133‑1 (MQ=255)                                                                                       cTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATAcc                                       >  2:22962/1‑133 (MQ=255)                                                                                          aGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGcc                                    <  2:347662/133‑1 (MQ=255)                                                                                            cgcATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGcccc                                  >  2:374700/1‑133 (MQ=255)                                                                                            cgcATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGcccc                                  >  2:610534/1‑133 (MQ=255)                                                                                                 ttCCGGAGCAATTCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAgg                             >  2:525023/1‑133 (MQ=255)                                                                                                             tCCAGATAGCTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGt                 <  1:22962/133‑1 (MQ=255)                                                                                                                      cTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATgg        >  1:552125/1‑133 (MQ=255)                                                                                                                      cTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATgg        >  2:82312/1‑133 (MQ=255)                                                                                                                      cTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATgg        >  1:598578/1‑133 (MQ=255)                                                                                                                      cTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATgg        >  2:478913/1‑133 (MQ=255)                                                                                                                      cTGTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATACGTTGATTCTTCGTGGTCTATgg        >  1:173823/1‑133 (MQ=255)                                                                                                                        gTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTAt          <  1:314708/129‑1 (MQ=255)                                                                                                                        gTCTATCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTAt          >  2:314708/1‑129 (MQ=255)                                                                                                                           tATCTTGTTTTCTGACTAAACTACCGGAACGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCAt   <  2:173823/133‑1 (MQ=255)                                                                                                                            aTCTTGTTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATc  >  2:386001/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                TAATGCCGCTGAATTTTTTCATTACCTCAGTTTCCTTTTCGTAAGTGCTCATTTTCGTTTCTGTATCCCAGCCCATATGATATTTGCTCAGCGCATTTCCGGAGCAATTCCAGATAGCTGTCTATCTTATTTCCTGAATAAACTACCGGATCGCCAGAAAGTGAGATTGCATAGTTAACTCTGTTATACATATTGAATACCGGCATACTTAAACATACCGCCCCATAGGTTGATTCTTCGTTGTCTATGGCCCATC  >  NC_000913/4524989‑4525244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑