Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	474,228	C→T	H421H (CAC→CAT)	amtB →	ammonia/ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	474,228	0	C	T	100.0%	68.5 / NA	21	H421H (CAC→CAT)	amtB	ammonia/ammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/9);  total (12/9)

GAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGC  >  NC_000913/474100‑474342                                                                                                                                 |                                                                                                                   gAAAGCATCGCCATTCCGATCGTCAGGTTCGGCGTTGTGGCTTTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCg                                                                                                                <  2:235551/133‑1 (MQ=255)   aaGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCgag                                                                                                              <  2:111772/133‑1 (MQ=255)    aGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCgaga                                                                                                             <  2:450925/133‑1 (MQ=255)      cATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAAt                                                                                                           >  2:411779/1‑133 (MQ=255)        tCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGc                                                                                                         >  2:343767/1‑133 (MQ=255)              ttACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATaa                                                                                                   <  1:356736/133‑1 (MQ=255)                 cGATCGTCTGGTCCGTTGGTGTTGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAAcgc                                                                                                <  1:206542/133‑1 (MQ=255)                     cGTCTGGTCCGGCGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAAAAAGCCTATAACGCGTaa                                                                                            >  1:222594/1‑133 (MQ=255)                              cGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTg                                                                                   >  1:109322/1‑133 (MQ=255)                                gtgtTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCa                                                                                 >  2:453940/1‑133 (MQ=255)                                           tttATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCg                                                                      >  2:478607/1‑133 (MQ=255)                                              aTCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGAc                                                                   >  2:12065/1‑133 (MQ=255)                                               tCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACg                                                                  <  2:625931/133‑1 (MQ=255)                                                   cTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGttt                                                              >  1:422194/1‑133 (MQ=255)                                                                gATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTa                                                 >  1:90343/1‑133 (MQ=255)                                                                     gACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTAttttt                                            <  1:411779/133‑1 (MQ=255)                                                                          ttGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAAt                                       <  2:422194/133‑1 (MQ=255)                                                                             gTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATtgt                                    >  1:370353/1‑133 (MQ=255)                                                                                tGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGAt                                 >  1:349487/1‑133 (MQ=255)                                                                                                  aGGAGCGAGAAGGGCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTGTTTAATTGTGAATAGGCATCATCCCTTCCt               >  1:170407/1‑133 (MQ=255)                                                                                                               gCTGGATGTCAACAGCCATGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGc  <  1:503459/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                   GAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAGAAGGGCTGGATGTCAACAGCCACGGCGAGAATGCCTATAACGCGTAACAAGCACTGCAAAAAACAGCCGGACGGTTTTCACCTCCGGCTATTTTTTTAATTGTGATTACGCATCACCCCTTCCTGAACGGTCGAGGC  >  NC_000913/474100‑474342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑