Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,920	C→A	T202T (ACC→ACA)	nadR →	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,920	0	C	A	100.0%	109.9 / NA	34	T202T (ACC→ACA)	nadR	DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/12);  total (22/12)

CGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAA  >  NC_000913/4627791‑4628046                                                                                                                                  |                                                                                                                               cGGCATCAAAAAGTTTATGGCT‑AAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTa                                                                                                                            >  1:522745/1‑133 (MQ=255)            aGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAg                                                                                                                  <  2:73590/133‑1 (MQ=255)                                  ccAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAAc                                                                                            <  2:130124/133‑1 (MQ=255)                                     gCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCg                                                                                         <  1:152894/133‑1 (MQ=255)                                              gatctACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTAc                                                                                <  2:441390/133‑1 (MQ=255)                                               atctACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACt                                                                               >  1:447692/1‑133 (MQ=255)                                                 ctACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTgg                                                                             <  2:522745/133‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCg                                                                                    >  2:445581/1‑123 (MQ=255)                                                    cacCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCg                                                                                    <  1:445581/123‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTAc                                                                                >  2:550955/1‑127 (MQ=255)                                                    cacCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTAc                                                                                <  1:550955/127‑1 (MQ=255)                                                    cacCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAAATCCGCGAAAATCCGTTCCGCTACTGGGaa                                                                          >  1:250131/1‑133 (MQ=255)                                                         cGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAatatat                                                                     >  1:345389/1‑133 (MQ=255)                                                         cGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAatatat                                                                     >  1:6892/1‑133 (MQ=255)                                                         cGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCCCTACTGAGAatatat                                                                     >  1:284101/1‑133 (MQ=255)                                                                          ccACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGc                                                    >  2:432677/1‑133 (MQ=255)                                                                                        aCATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGt                                      >  1:476995/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    >  1:547492/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    >  1:507257/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    >  2:117792/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    >  1:465467/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGAGAATATATGCCTGCCGAAGTGAAGCTGTTTGTTGTGCGTAc                                    >  2:163990/1‑133 (MQ=255)                                                                                          aTCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGAGAATATATCCCTGCCGTAGTGAAGCGGTTTTTTGTGCGTAc                                    >  2:174039/1‑133 (MQ=255)                                                                                            cTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGGATATATTCCTACCGTACAGAAGCCGTTTTTTGTGCGTAcgg                                  >  2:287934/1‑131 (MQ=255)                                                                                            cTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    <  1:554155/131‑1 (MQ=255)                                                                                            cTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTAc                                    >  2:554155/1‑131 (MQ=255)                                                                                            cTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCg                                  >  2:464752/1‑133 (MQ=255)                                                                                                                 gTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGt             >  1:155276/1‑133 (MQ=255)                                                                                                                 gTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGt             >  2:392646/1‑133 (MQ=255)                                                                                                                 gTCGATCCGAAACGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGt             >  1:331519/1‑133 (MQ=255)                                                                                                                      tCCGAAACCTACATTCATCAGTTTCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGc        <  1:174039/133‑1 (MQ=255)                                                                                                                         gAAACGTACATTTATGAGTATCAGGGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGt     <  2:250131/133‑1 (MQ=255)                                                                                                                           atcGTACATTCATGAATATCAGCGGTGCCCAGATCCGCGAACACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTaa   <  2:284101/131‑1 (MQ=255)                                                                                                                            aCGTACATTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTaaa  <  1:117792/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                               CGGCATCAAAAAGTTTATGGCTGAAAAAGGGATCCAGCCGGATCTGATCTACACCTCGGAAGAAGCCGATGCGCCACAGTATATGGAACATCTGGGGATCGAGACGGTGCTGGTCGATCCGAAACGTACCTTTATGAGTATCAGCGGTGCGCAGATCCGCGAAAACCCGTTCCGCTACTGGGAATATATTCCTACCGAAGTGAAGCCGTTTTTTGTGCGTACCGTGGCGATCCTTGGCGGCGAGTCGAGCGGTAAA  >  NC_000913/4627791‑4628046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑