Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	485,852	C→T	S31F (TCC→TTC)	acrR →	DNA‑binding transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	485,852	0	C	T	100.0%	170.2 / NA	50	S31F (TCC→TTC)	acrR	DNA‑binding transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/28);  total (22/28)

ATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTA  >  NC_000913/485724‑485979                                                                                                                                 |                                                                                                                                atgtatgtAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTAGGTCTTTTGTCACAGCAAGGGGGATCATTCAcc                                                                                                                             >  2:176143/1‑133 (MQ=255) atgtatgtAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTAGGTCATTTATCACAGCATGGGGTATCATTCAcc                                                                                                                             >  2:247137/1‑133 (MQ=255)   gtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTTGCTCTACGGCTTGTCTAACAGCAGGAGGTAGCATTCACCTc                                                                                                                           >  2:173023/1‑133 (MQ=255)   gtatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTc                                                                                                                           >  1:459592/1‑133 (MQ=255)    tatgtaAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAGGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCg                                                                                                                          <  1:455462/133‑1 (MQ=255)         aaaTCTAACGCCTGTTAAGTCACGAACATAGGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTggg                                                                                                                     <  1:176143/133‑1 (MQ=255)           aTCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCg                                                                                                                   >  1:59316/1‑133 (MQ=255)             cTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCgag                                                                                                                 >  2:554515/1‑133 (MQ=255)                aCGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTGTCTCACAGCAGGGGATATCATTCACCTCGCTGGGCGAGAtt                                                                                                              >  2:273651/1‑133 (MQ=255)                 cGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCGTTCCCCCCGCTGGACGAGATTg                                                                                                             >  1:205718/1‑133 (MQ=255)                     tGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCaaa                                                                                                         >  1:154672/1‑133 (MQ=255)                     tGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCaaa                                                                                                         >  2:546169/1‑133 (MQ=255)                     tGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCaaa                                                                                                         <  1:465251/133‑1 (MQ=255)                      gTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCaaaa                                                                                                        >  1:100550/1‑133 (MQ=255)                       tAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAag                                                                                                       >  2:547765/1‑133 (MQ=255)                       tAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAag                                                                                                       >  2:370973/1‑133 (MQ=255)                                      atGGCACGAAAAACCTAACAAGAAGCGCCAGAAACGCGCCACCACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgc                                                                                        <  2:247460/133‑1 (MQ=255)                                      atGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgc                                                                                        <  1:22007/133‑1 (MQ=255)                                       tGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                       <  1:69873/133‑1 (MQ=255)                                       tGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                       <  2:341494/133‑1 (MQ=255)                                       tGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTAcgcg                                                                                       <  1:247137/133‑1 (MQ=255)                                            cGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTg                                                                                  >  1:544193/1‑133 (MQ=255)                                              aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                                                                                <  1:546169/133‑1 (MQ=255)                                              aaaaaCCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCa                                                                                <  1:554515/133‑1 (MQ=255)                                                     aaaCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACt                                                                         <  2:325674/133‑1 (MQ=255)                                                       aCAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTgg                                                                       >  2:419827/1‑133 (MQ=255)                                                        cAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGc                                                                      <  2:565337/133‑1 (MQ=255)                                                             agCGCAAGCAACGCGCCACCACCTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAtttt                                                                 <  1:185537/133‑1 (MQ=255)                                                                 cAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTTCGCGCGGTGCAATCAACTGGCTTTTTAAAg                                                             >  1:201178/1‑133 (MQ=255)                                                                       aCGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGt                                                       <  1:47081/133‑1 (MQ=255)                                                                            ccAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGAt                                                  <  1:88154/133‑1 (MQ=255)                                                                                cacaTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                              <  1:370973/133‑1 (MQ=255)                                                                                cacaTCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGt                                              <  2:59316/133‑1 (MQ=255)                                                                                     ccTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                         <  1:419827/133‑1 (MQ=255)                                                                                     ccTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGt                                         <  2:394713/133‑1 (MQ=255)                                                                                      cTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTg                                        <  2:557995/133‑1 (MQ=255)                                                                                       tCGATGTGGCTCTACTTCTTTTCTTACATCAGGGGGTATCAATCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTga                                       <  2:201178/133‑1 (MQ=255)                                                                                       tCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTga                                       >  2:505626/1‑133 (MQ=255)                                                                                           tgtgGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCCACTGGCATCTTAAAGAGAAGTCGGATTTGTTCAGTGAGATc                                   >  2:241798/1‑133 (MQ=255)                                                                                              ggCTCTACATCTTTTTTCGCAGTAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTgg                                <  1:273651/133‑1 (MQ=255)                                                                                               gCTCTACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTggg                               >  2:612158/1‑133 (MQ=255)                                                                                                   tACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACt                           >  2:10315/1‑133 (MQ=255)                                                                                                   tACGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCAGTTTAAAGATAAGTCAGATTTGTTCATTGAGATCTGGGAACt                           >  2:274943/1‑133 (MQ=255)                                                                                                     cGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGt                         <  2:459592/133‑1 (MQ=255)                                                                                                     cGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGt                         >  1:82758/1‑133 (MQ=255)                                                                                                     cGTCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGt                         <  1:589567/133‑1 (MQ=255)                                                                                                       tCTTTTCTCACAGCAGGGGGTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCa                       <  2:556405/133‑1 (MQ=255)                                                                                                                acaGCAGGAGGTATCCTTCACCCCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAat              <  1:241798/133‑1 (MQ=255)                                                                                                                       ggggTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGGCAGAATCCAGTATTGGTg       >  1:303600/1‑133 (MQ=255)                                                                                                                          gTATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAAc    <  2:544193/133‑1 (MQ=255)                                                                                                                           tATCATTCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACt   <  2:400233/133‑1 (MQ=255)                                                                                                                            atcattCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCGGGGAACTGTCAGAATCTAATAGTGGTGAACTa  >  2:178853/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                                ATGTATGTAAATCTAACGCCTGTAAATTCACGAACATATGGCACGAAAAACCAAACAAGAAGCGCAAGAAACGCGCCAACACATCCTCGATGTGGCTCTACGTCTTTTCTCACAGCAGGGGGTATCATCCACCTCGCTGGGCGAGATTGCAAAAGCAGCTGGCGTTACGCGCGGTGCAATCTACTGGCATTTTAAAGACAAGTCGGATTTGTTCAGTGAGATCTGGGAACTGTCAGAATCCAATATTGGTGAACTA  >  NC_000913/485724‑485979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑