Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	510,996	A→C	T128T (ACT→ACG)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	510,996	0	A	C	100.0%	62.4 / NA	19	T128T (ACT→ACG)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/10);  total (9/10)

GGCGTTTAGCGTCATCTTCAATCGCTTCCGCGCCGTAGCCCGCTTTTTCCACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCT  >  NC_000913/510869‑511117                                                                                                                                |                                                                                                                          ggcgTTTAGCGTCATCTTCAATCGCTTCCGCGCCGTAGCCCGCTTTTTCCACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGAc                                                                                                                      <  2:116579/133‑1 (MQ=255)                   cAATCGCTGCCGCGCCGTCGCCTGCTTTTTTCACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAg                                                                                                    <  1:183693/133‑1 (MQ=255)                         cTTCCGCGCCGTAGCCCGCTTTTTCCACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCAtt                                                                                              >  1:140254/1‑133 (MQ=255)                                            tttttCCACCGCCTGAACGAAAACTTGTGGAGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAg                                                                           <  1:302393/133‑1 (MQ=255)                                                  cACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGgcg                                                                     <  2:140254/133‑1 (MQ=255)                                                     cGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAg                                                                  >  2:606078/1‑133 (MQ=255)                                                       ccTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTATGCAGCGCTTTTTGTACGCGGGTGACACAGATGGCGCAGCt                                                                >  2:194954/1‑133 (MQ=255)                                                        cTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTAGCAGCGCATTTTGTACGCGGGTGACCCAGCGGGCGCAGCTc                                                               >  2:228781/1‑133 (MQ=255)                                                           cACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATg                                                            <  2:548178/133‑1 (MQ=255)                                                                     tgtgGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTcagca                                                  >  2:12172/1‑133 (MQ=255)                                                                     tgtgGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTcagca                                                  >  2:32669/1‑133 (MQ=255)                                                                          ggAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAAc                                             >  1:31606/1‑133 (MQ=255)                                                                                aCTGCCCAGCACCAGAGCTGTACGCTCCGCGAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTgctgg                                       <  1:194954/133‑1 (MQ=255)                                                                                        tCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACACGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAActgct                                         >  1:464427/1‑123 (MQ=255)                                                                                        tCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACACGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAActgct                                         <  2:464427/123‑1 (MQ=255)                                                                                          aCCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTgtcatcgt                             <  1:132543/133‑1 (MQ=255)                                                                                                                   ttACCCGTGCATGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAgcgc    <  2:37076/133‑1 (MQ=255)                                                                                                                    tACCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGcc   <  2:566137/133‑1 (MQ=255)                                                                                                                     aCCCGTGCCTGCGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCAACGTCATCCGCGATCACTGCCGAAAGCGCCt  >  1:213435/1‑133 (MQ=255)                                                                                                                                |                                                                                                                          GGCGTTTAGCGTCATCTTCAATCGCTTCCGCGCCGTAGCCCGCTTTTTCCACCGCCTGCACTAAATCTTGTGGGGAGGCACTGCCCATCACCAGCGCAGTACGCTCCGCCAGGTTTACCCGTGCCTGAGTGACGCCCGGTACGCTTTGCAGCGCATTTTGTACGCGGGTGACACAGCTGGCGCAGCTCATGCCGCTCAGCAGCAACTGCTGGCTGTCATCGTCATCGGCGGTCGCTGCCGGAAGCGCCT  >  NC_000913/510869‑511117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑