Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	669,942	A→G	L211L (TTG→CTG)	nadD ←	nicotinate‑mononucleotide adenylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	669,942	0	A	G	100.0%	128.9 / NA	38	L211L (TTG→CTG)	nadD	nicotinate‑mononucleotide adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/15);  total (23/15)

CGCGCGGTCA‑GGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTT  >  NC_000913/669819‑670070                                                                                                                              |                                                                                                                                 cgcgcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACCGGTATCAGGGATAAAGGCCttgtt                                                                                                                          >  2:243206/1‑133 (MQ=255) cgcgcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCttgtt                                                                                                                          >  2:310150/1‑133 (MQ=255) cgcgcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCttg                                                                                                                            >  2:375632/1‑131 (MQ=255) cgcgcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCttg                                                                                                                            <  1:375632/131‑1 (MQ=255)    gcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGt                                                                                                                       <  1:310150/133‑1 (MQ=255)    gcgGTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTAACAGTGAGACAGGCCTTGTTGGt                                                                                                                       >  1:295900/1‑133 (MQ=255)    gcgGTCA‑GGGTGGGTCGGCGCATGCCCGCTGTCAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGt                                                                                                                       <  2:176596/133‑1 (MQ=255)      ggTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTa                                                                                                                     >  2:151692/1‑133 (MQ=255)      ggTCAGGGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTa                                                                                                                     >  1:110803/1‑133 (MQ=255)         cagggg‑ggaTGGGCGTATCACCCCTGTAAATACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATg                                                                                                                  <  1:293203/130‑1 (MQ=255)          agggg‑gggTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGt                                                                                                                 <  2:479994/131‑1 (MQ=255)                   tGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGTTACAGGCTTTGTTGGTTAATGTAAGTCAGt                                                                                                         >  1:234678/1‑133 (MQ=255)                   tGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGt                                                                                                         >  1:13947/1‑133 (MQ=255)                          aTGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGtt                                                                                                  <  1:346589/133‑1 (MQ=255)                                           cATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTca                                                                                 <  2:110803/133‑1 (MQ=255)                                            aTCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTcac                                                                                <  2:453975/133‑1 (MQ=255)                                             tCGATATTAGCTTGCTTTTCACCATGACGACTTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTcaca                                                                               <  1:166272/133‑1 (MQ=255)                                                   ttCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCCGCAATAAATCCTCACATGATTc                                                                         >  1:238124/1‑133 (MQ=255)                                                        ttGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGt                                                                    >  2:56052/1‑133 (MQ=255)                                                        ttGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAACGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGt                                                                    >  2:432013/1‑133 (MQ=255)                                                           cGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTTACATGATTCACCGtttt                                                                 >  1:146916/1‑133 (MQ=255)                                                                      gACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTcg                                                      >  1:587727/1‑133 (MQ=255)                                                                      gACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTcg                                                      >  2:127747/1‑133 (MQ=255)                                                                      gACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTcg                                                      >  1:563147/1‑133 (MQ=255)                                                                              aaCCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGgatgat                                              <  2:313837/133‑1 (MQ=255)                                                                                    aGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGc                                        >  2:71112/1‑133 (MQ=255)                                                                                     gTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGt                                           >  2:518548/1‑129 (MQ=255)                                                                                     gTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGt                                           <  1:518548/129‑1 (MQ=255)                                                                                      tCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCg                                      <  1:127747/133‑1 (MQ=255)                                                                                           tcatGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATg                                 >  1:92217/1‑133 (MQ=255)                                                                                              tGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCg                                      <  2:94115/125‑1 (MQ=255)                                                                                              tGCCCTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCg                                      >  1:94115/1‑125 (MQ=255)                                                                                                cccTCCGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTaaa                            >  2:11359/1‑133 (MQ=255)                                                                                                   tgcGTAACGCCAGGTCTCAGCGATTCTGCCCTAGTTGGTTACTGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCa                         <  2:295900/131‑1 (MQ=255)                                                                                                     cGTAACGACAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACg                       >  1:576935/1‑133 (MQ=255)                                                                                                             cAGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCa               >  2:611673/1‑133 (MQ=255)                                                                                                              aGGTATCAGCGATACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAg              <  2:13947/133‑1 (MQ=255)                                                                                                                          tACAGGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAAttt  >  2:443463/1‑133 (MQ=255)                                                                                                                              |                                                                                                                                 CGCGCGGTCA‑GGG‑GGGTGGGCGCATGACCGCTGTAAAGACCATCGATATTCGCTTGCGTTTCACCATGACGAATTAACCACAGTCGCATCATGCCCTCCGTAACGACAGGTATCAGCGATACAAGCCTTGTTGGTTAATGTAAGTCAGTACCGGTTCCGGCAATAAATCCTCACATGATTCACCGTTTTGCAAACGTTCGCGGATGATGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGATAAATTT  >  NC_000913/669819‑670070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑