Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	742,864	A→G	K13R (AAA→AGA)	ybgI →	radiation resistance protein YbgI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	742,864	0	A	G	100.0%	102.5 / NA	30	K13R (AAA→AGA)	ybgI	radiation resistance protein YbgI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

ATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAAACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAG  >  NC_000913/742745‑742983                                                                                                                        |                                                                                                                        atGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGTAACGACTGATTAACGAGAGACGGAACAGcgcg                                                                                                            >  2:193209/1‑133 (MQ=255) atGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGATAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGcgcg                                                                                                            <  2:336588/133‑1 (MQ=255)         aGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTAGAACAACTGATTAACGAAAGACTGAGCAGCGCGGCGATTAg                                                                                                    >  2:178795/1‑133 (MQ=255)                cTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTAc                                                                                             <  2:357359/133‑1 (MQ=255)                       cgaACTGGTGAGGTAACGATATTTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGa                                                                                      <  1:234006/133‑1 (MQ=255)                       cgaACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGa                                                                                      <  2:424513/133‑1 (MQ=255)                        gaACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGaa                                                                                     <  1:50842/133‑1 (MQ=255)                              gCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGttt                                                                               >  1:357725/1‑133 (MQ=255)                                       cGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGa                                                                      <  2:322373/133‑1 (MQ=255)                                                 aCGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACACGCTGTATGGTTTGCAGGTGGACGGCAAagag                                                            >  2:274340/1‑133 (MQ=255)                                                      gtAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAGTGGTTTGTAGGTGGTAGGCAACGAGACGGt                                                       >  1:232504/1‑133 (MQ=255)                                                      gtAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGt                                                       <  1:363305/133‑1 (MQ=255)                                                      gtAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGt                                                       <  2:357725/133‑1 (MQ=255)                                                        aGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGt                                                       <  2:352841/131‑1 (MQ=255)                                                        aGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGt                                                       >  1:352841/1‑131 (MQ=255)                                                               aGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAAt                                              >  1:11118/1‑133 (MQ=255)                                                               aGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAAt                                              >  1:523496/1‑133 (MQ=255)                                                                 aaTACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTAGAAGGCAAAGAGCCGGTACAAAAAATTg                                            >  2:246418/1‑133 (MQ=255)                                                                   tACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGtt                                          >  1:360307/1‑133 (MQ=255)                                                                           gcgcGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGgtgt                                  >  1:6320/1‑133 (MQ=255)                                                                           gcgcGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAAGTGGAAGGAAAATAAACTGTGCAAAAAATTGATACCGgtgt                                  >  2:299050/1‑133 (MQ=255)                                                                             gcgAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCa                                >  2:332918/1‑133 (MQ=255)                                                                                aaGATGAAGAACACCCAATTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCg                             <  1:299050/133‑1 (MQ=255)                                                                                     gAAAAACACCGAACTTGAACAACTGATTAAAGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCgccagc                        <  1:208490/133‑1 (MQ=255)                                                                                           cacCGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTATTACCGGTGTCACCGCCAGCCAGGCt                  >  1:610818/1‑133 (MQ=255)                                                                                             ccGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTCTGAACGGTGTCAACGCCCGCCAGGCttt                >  2:290238/1‑133 (MQ=255)                                                                                              cGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGTAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTg               >  2:559724/1‑133 (MQ=255)                                                                                              cGAACTGGAACAACTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTg               <  2:349523/133‑1 (MQ=255)                                                                                                           cTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAg  >  2:9358/1‑133 (MQ=255)                                                                                                           cTGATTAACGAAAGACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAg  >  2:96546/1‑133 (MQ=255)                                                                                                                        |                                                                                                                        ATGTCAAAAGATTGGCTATCGACGAACTGGCGAGGTAACGCTATGTTAACGGTGTAGCTATCAGAATACGGAAGGCGCGAAGATGAAAAACACCGAACTGGAACAACTGATTAACGAAAAACTGAACAGCGCGGCGATTAGTGATTACGCGCCGAATGGTTTGCAGGTGGAAGGCAAAGAGACGGTGCAAAAAATTGTTACCGGTGTCACCGCCAGCCAGGCTTTGCTCGATGAGGCAG  >  NC_000913/742745‑742983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑