Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 784,092 T→G V244V (GTA→GTC)  zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913784,0920TG100.0% 147.8 / NA 44V244V (GTA→GTC) zitBZn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/17);  total (27/17)

GATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATCTTTCAACAGTCGCCA  >  NC_000913/783983‑784220
                                                                                                             |                                                                                                                                
gATAGTGATCTATCATGTAGTGTTTGATCTGACCCAACAAGACATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCg                                                                                                           <  2:158705/133‑1 (MQ=255)
     tGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACtt                                                                                                      >  1:450515/1‑133 (MQ=255)
       aTCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTcc                                                                                                    >  2:488878/1‑133 (MQ=255)
       aTCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGCTGGACATTGAGGACTTcc                                                                                                    >  1:265314/1‑133 (MQ=255)
           aTCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGa                                                                                                >  2:440255/1‑133 (MQ=255)
           aTCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGAGGCACATTGCGGACTTCCGGGa                                                                                                >  1:204607/1‑133 (MQ=255)
               ggTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTc                                                                                            >  2:49658/1‑133 (MQ=255)
                    tgtTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGAAATGTACATGGTATACATTGCGGACTACCGGGATTTCCCGGc                                                                                       >  2:227383/1‑133 (MQ=255)
                      ttGGATCTGGTCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGcac                                                                                     >  2:495935/1‑133 (MQ=255)
                      ttGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGGACATTGCAGACGTCGGGGATTACCCGGcac                                                                                     >  1:239799/1‑133 (MQ=255)
                         gATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATa                                                                                  >  2:581606/1‑133 (MQ=255)
                          aTCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATac                                                                                 >  1:605435/1‑133 (MQ=255)
                            cTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATacga                                                                               <  1:581606/133‑1 (MQ=255)
                              gATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATacgacg                                                                             >  1:315222/1‑133 (MQ=255)
                              gATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATacgacg                                                                             >  1:331827/1‑133 (MQ=255)
                              gATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATacgacg                                                                             >  2:498087/1‑133 (MQ=255)
                                   aacaaGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCttca                                                                        >  1:539991/1‑133 (MQ=255)
                                    acaaGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCttcag                                                                       >  2:403833/1‑133 (MQ=255)
                                    acaaGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCttcag                                                                       >  1:144585/1‑133 (MQ=255)
                                      aaGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCttcagtt                                                                     <  2:331827/133‑1 (MQ=255)
                                           atcgtgatcgtgTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCg                                                                >  2:36521/1‑133 (MQ=255)
                                               tgatcgtgTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGatat                                                            <  1:440255/133‑1 (MQ=255)
                                                 atcgtgTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATcc                                                          <  2:315222/133‑1 (MQ=255)
                                                   cgtgTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAg                                                        <  1:495935/133‑1 (MQ=255)
                                                      gtgGCGGGATCACCTTCACATGCAGCGTCATCCCCGGCTTCCCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGa                                                     <  2:239799/133‑1 (MQ=255)
                                                      gtgGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGa                                                     <  2:450515/133‑1 (MQ=255)
                                                       tgGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGAt                                                    >  1:42786/1‑133 (MQ=255)
                                                       tgGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGAt                                                    <  1:36521/133‑1 (MQ=255)
                                                        ggagggATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTGAGTGCATCGATATGCAGCGATa                                                   >  1:290703/4‑133 (MQ=255)
                                                         gCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATAc                                                  <  1:49658/133‑1 (MQ=255)
                                                         gCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATAc                                                  >  2:33741/1‑133 (MQ=255)
                                                              aTCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTg                                             <  1:39321/133‑1 (MQ=255)
                                                               tCACCTGCACATGCAACGTCATCACCGGCTTCTCCCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGGTTTCCCGGCACATACGACGCTTCGATTGAGCGATATACCGCAATACCGGTGc                                            >  2:221494/1‑133 (MQ=255)
                                                                cACCTGCACACGCAACGTCATCACCGGCATCTTGCCTACCAACCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCa                                           <  2:204607/133‑1 (MQ=255)
                                                                 aCCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTCCAGGTCAGCGATATCCAGCGACACCGGTGCAc                                          >  2:266438/1‑133 (MQ=255)
                                                                   cTGCACATGCCACGTTATCACCGGGTTCCCGCATACCATCCCGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCt                                        <  2:218810/133‑1 (MQ=255)
                                                                           gCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTaa                                >  1:626751/1‑133 (MQ=255)
                                                                           gCAACGTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCAATACCGATGCACCTTCAAGTaa                                >  1:231050/1‑133 (MQ=255)
                                                                                gTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCGGCGATACGGGTTCAACTTCAACTAattcat                           >  1:163345/1‑133 (MQ=255)
                                                                                gTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAattcat                           >  1:522284/1‑133 (MQ=255)
                                                                                gTCATCACCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTCCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAattcat                           <  2:378695/133‑1 (MQ=255)
                                                                                   atcaCCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAAttcattca                        <  2:144585/133‑1 (MQ=255)
                                                                                      aCCGGCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGc                     <  2:468771/133‑1 (MQ=255)
                                                                                          gCTTCTCGCCTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATc                 >  1:443368/1‑133 (MQ=255)
                                                                                                  ccTACCATCCAGACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATCTTTCAACa         <  1:387869/133‑1 (MQ=255)
                                                                                                        atccagACATGTACATGGTGCACATTGCGGACTTCCGGGATTTTCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATCTTTCAACAGTCGcc   <  2:31207/133‑1 (MQ=255)
                                                                                                         tccagACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATCTTTCAACAGTCGCCa  >  2:36002/1‑133 (MQ=255)
                                                                                                             |                                                                                                                                
GATAGTGATCCATCAGGTAGTGTTGGATCTGATCCAACAAGGCATCGTGATCGTGTGGCGGGATCACCTGCACATGCAACGTCATCACCGGCTTCTCGCCTACCATCCATACATGTACATGGTGCACATTGCGGACTTCCGGGATTTCCCGGCACATACGACGCTTCAGTTCAGCGATATCCAGCGATACCGGTGCACCTTCAAGTAATTCATTCACGCTATCTTTCAACAGTCGCCA  >  NC_000913/783983‑784220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑