| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 830,378 | T→C | Q89R (CAG→CGG) | cecR ← | DNA‑binding transcriptional dual regulator CecR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 830,378 | 0 | T | C | 96.3% | 85.7 / NA | 27 | Q89R (CAG→CGG) | cecR | DNA‑binding transcriptional dual regulator CecR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (18/8); minor base A (1/0); total (19/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCTGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGAAGTAGTAGGTGATGG > NC_000913/830263‑830504 | gagCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAg > 2:605834/1‑133 (MQ=255)gagCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAg > 1:395114/1‑133 (MQ=255) ctCACGGGAGATAAACTTGCTGAGGTTGGCGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGGTCAGTTCACGGGTGGCAGCCGGATTCAGAC‑GTGGTTGAGCGACAAAGCGttag > 2:299126/1‑131 (MQ=255) gggAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCCGCCCGA‑TCCGGCAGTGCTTGCGCGAACAAGGGTTCGGCTTc > 2:199353/1‑133 (MQ=255) aaCTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCAt < 2:570285/133‑1 (MQ=255) aaCTTGCTGAGGTTGACGGTGTCACCCTGGGTCAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCAt < 2:186367/133‑1 (MQ=255) gCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGcgga > 2:577138/1‑133 (MQ=255) gCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGcgga > 1:578262/1‑133 (MQ=255) ggTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGaa > 1:324695/1‑133 (MQ=255) aCGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTCCGGCTTCGTCGTCATGCGGACGGAACTGCt > 1:249633/1‑133 (MQ=255) tCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCaa > 2:85419/1‑133 (MQ=255) tCATCCAGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCaa > 2:455808/1‑133 (MQ=255) tGGGTGAGCAGCTTATTCTTGTTCCTGCAGTCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAAt < 1:199353/133‑1 (MQ=255) ggTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCg > 1:39743/1‑133 (MQ=255) ggTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCg > 1:376701/1‑133 (MQ=255) ggTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCCGTTGGAACTGTTCGCCAATAAAAtag > 2:287930/1‑131 (MQ=255) tGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGc > 1:399983/1‑133 (MQ=255) cTTAATCACGTTCCTGCATGCGTGAAGGATAAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCAc < 1:287930/133‑1 (MQ=255) ttCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACAGAACTGCTCGCCGATAAAATCGGCAGTCCACTGGGCGCAgg > 2:260481/1‑133 (MQ=255) tCCTGCAGGCACGAAGGATCAGTTTACGGATGGCAGCCCGA‑ACAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGc < 2:246498/133‑1 (MQ=255) cgAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAAt > 1:342708/1‑133 (MQ=255) cgAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAAt > 1:20653/1‑133 (MQ=255) cgAAGGATCAATTCACGGATGGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAAt > 1:495294/1‑133 (MQ=255) gTTCACGGCTGGCAGCCCAA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGa < 1:299126/133‑1 (MQ=255) cGGATGGCTGCCCGA‑TCATGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGa < 1:257764/133‑1 (MQ=255) cGGATAGCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGa > 2:78972/1‑133 (MQ=255) gCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTTGAACCGAag > 2:394180/1‑129 (MQ=255) gCAGCCCGA‑TCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGAag < 1:394180/129‑1 (MQ=255) tCAGGCCGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGAAGTAGTAGGTGATgg < 1:605834/133‑1 (MQ=255) | GAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCTGCAGGCGCGAAGGATCAGTTCACGGATGGCAGCCCGA‑TCAGGCTGTGGTTGCGCGAACAAGCGTTCGGCTTCCTCGGCATGCGGACGGAACTGCTCGCCAATAAAATCGGCAATCCACTGGGCGCAGGCGAGGTACAAATCTTCTTTCGAACCGAAGTAGTAGGTGATGG > NC_000913/830263‑830504 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |