Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 858,226 A→G V279A (GTG→GCG)  ybiU ← DUF1479 domain‑containing protein YbiU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913858,2260AG97.5% 137.8 / NA 40V279A (GTG→GCG) ybiUDUF1479 domain‑containing protein YbiU
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (25/14);  total (26/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCA  >  NC_000913/858115‑858354
                                                                                                               |                                                                                                                                
tGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGAc                                                                                                             >  2:1338/1‑133 (MQ=255)
    ctGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAgg                                                                                                         <  1:325300/133‑1 (MQ=255)
       aTACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCa                                                                                                           >  1:133424/1‑128 (MQ=255)
       aTACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCa                                                                                                           <  2:133424/128‑1 (MQ=255)
       aTACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGgcag                                                                                                      >  2:534904/1‑133 (MQ=255)
       aTACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGgcag                                                                                                      >  1:132398/1‑133 (MQ=255)
       aTACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCGTCAGGAATAGGCACTATTTGCAGCACCCCCCGACCAGgcgg                                                                                                      >  2:294602/1‑131 (MQ=255)
            ggCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCatat                                                                                                 >  1:459431/1‑133 (MQ=255)
                acaCTCTTCCCGGCGGTACGCCGCTCAGTTCAACCTCCGGCTCATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCaga                                                                                             <  2:288565/131‑1 (MQ=255)
                 tACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCagag                                                                                            >  2:509327/1‑133 (MQ=255)
                      ttCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGAGCAGTCATCATATCTGAGAgcgc                                                                                       >  1:288565/1‑133 (MQ=255)
                          gggCGCTATGCCGCATAGATCATCCTCCGGTACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTc                                                                                   <  1:294602/133‑1 (MQ=255)
                          gggCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTc                                                                                   <  1:509327/133‑1 (MQ=255)
                          gggCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTc                                                                                   <  1:534904/133‑1 (MQ=255)
                           ggCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTcc                                                                                  >  1:562342/1‑133 (MQ=255)
                             cgcTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTc                                                                                   >  1:458216/1‑130 (MQ=255)
                             cgcTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTc                                                                                   <  2:458216/130‑1 (MQ=255)
                              gcTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGc                                                                               >  1:359462/1‑133 (MQ=255)
                                         caGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGAGCAGGCAGCATATAAGAGAGCGCTGTTCAGCCCTGGAATGTc                                                                    >  2:252008/1‑133 (MQ=255)
                                              cATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGaaa                                                               >  2:523159/1‑133 (MQ=255)
                                                 cctccGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGATAGCCCTGTCCAGCCCTGGAATCTCCGAAacac                                                            >  2:213348/1‑133 (MQ=255)
                                                 cctccGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCg                                                                  >  1:393558/1‑127 (MQ=255)
                                                 cctccGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCg                                                                  <  2:393558/127‑1 (MQ=255)
                                                  ctccGGCACATCATCAAGCAGCGGTCGTGACACTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACg                                                           <  2:241201/133‑1 (MQ=255)
                                                  ctccGGCACAACATCAAGCAGCGGCCGTGACATTACGTACGGCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACg                                                           <  2:194032/133‑1 (MQ=255)
                                                     cGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGaa                                                        >  2:39718/1‑133 (MQ=255)
                                                      ggCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAAc                                                       >  1:330341/1‑133 (MQ=255)
                                                      ggCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATAAGAGAGCGATGTCCAGCCCTGGAATGTCCGGAACACGGAAc                                                       >  2:239972/1‑133 (MQ=255)
                                                                       cGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTGCGAAACAAGGATCATTTGGTGGTGTTGTcc                                      >  1:173181/1‑133 (MQ=255)
                                                                         gACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGAGGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCTAAACACGAAACATTGGGTTGTATTGTCCAc                                    >  1:203828/1‑133 (MQ=255)
                                                                          aCGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCAcc                                   <  2:459431/133‑1 (MQ=255)
                                                                             tAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCgtg                                <  2:132398/133‑1 (MQ=255)
                                                                             tAACAGTACGTACGCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCgtg                                <  2:330341/133‑1 (MQ=255)
                                                                                      gtacgCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCa                       >  2:104310/1‑133 (MQ=255)
                                                                                        acgCCATAGCTTCAGGAATGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTAGGTGGTGATGTGCAACGTGTAATCTTCAAc                     >  1:175372/1‑133 (MQ=255)
                                                                                                         aTGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCTTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCg    <  2:465003/133‑1 (MQ=255)
                                                                                                         aTGGGCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCg    >  1:377755/1‑133 (MQ=255)
                                                                                                           gggCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCa  >  2:340879/1‑133 (MQ=255)
                                                                                                           gggCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCa  >  2:34498/1‑133 (MQ=255)
                                                                                                           gggCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCa  >  1:93930/1‑133 (MQ=255)
                                                                                                           gggCGCGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCa  <  1:34498/133‑1 (MQ=255)
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TGCTCTGATACCGGCAATACTCTTCCGGGCGCTACGCCGCACAGTTCATCCTCCGGCACATCATCAAGCAGCGGACGTAACAGTACGTACGCCATAGCTTCAGGAATGGGCACGACGTGCAGCAGCCCCTGACCAGGCAGCATATCAGAGAGCGCTGTCCAGCCCTGGAATGTCCGAAACACGGAACATTTGGTGGTGTTGTCCACCGTGTACTCTTCAACTTCCGTACGATGTGCCGCA  >  NC_000913/858115‑858354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑