Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	874,796	A→G	R606R (AGA→AGG)	pdeI →	putative c‑di‑GMP phosphodiesterase PdeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	874,796	0	A	G	100.0%	99.4 / NA	28	R606R (AGA→AGG)	pdeI	putative c‑di‑GMP phosphodiesterase PdeI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/12);  total (16/12)

TTGTGCCGTTGGGTGTCTGCGGAGCGGGGGATCATTTCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGACATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTACCACCATCATGAAAGAAAATGGCCTG  >  NC_000913/874668‑874919                                                                                                                                 |                                                                                                                            ttGTGCCGTTGGGTGCCTGCGGAGTGGGGGATTATTTCACCACTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCAtt                                                                                                                         <  2:187861/133‑1 (MQ=255)      ccGTTGGGTGTCTGCGGAGCGGGGGATCATTTCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGt                                                                                                                    >  1:385224/1‑133 (MQ=255)                              gATCATTTCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATAAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCACTTTAGTCGGCGGGCTTCGCTGAAGgatgat                                                                                            >  1:182625/1‑133 (MQ=255)                                    ttCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTc                                                                                         <  1:120904/130‑1 (MQ=255)                                    ttCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTc                                                                                         >  2:120904/1‑130 (MQ=255)                                    ttCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTa                                                                                      <  1:110184/133‑1 (MQ=255)                                      cACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACt                                                                                    <  1:478484/133‑1 (MQ=255)                                      cACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACt                                                                                    <  2:446600/133‑1 (MQ=255)                                      cACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACt                                                                                    >  2:478484/1‑133 (MQ=255)                                       aCCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTg                                                                                   <  1:73939/133‑1 (MQ=255)                                         cGCTGAAGTTCATTATCATTGCTGAAGATATCTGGTTTATATATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCa                                                                                 <  2:182625/133‑1 (MQ=255)                                            tGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCatat                                                                              >  1:611607/1‑133 (MQ=255)                                              aaGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTa                                                                            <  2:611607/133‑1 (MQ=255)                                                   cATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGTTTTCTTACTGCATATTAATGtt                                                                       >  1:209978/1‑133 (MQ=255)                                                           ttGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTg                                                               >  1:530279/1‑133 (MQ=255)                                                              cTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCa                                                            >  2:10176/1‑133 (MQ=255)                                                                        cGGGTTTATCAATGAACTGGGTTACCAGGTTACTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGaa                                                  <  2:209978/133‑1 (MQ=255)                                                                           gTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACca                                               >  2:626182/1‑133 (MQ=255)                                                                                    tGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCAt                                      <  2:385224/133‑1 (MQ=255)                                                                                        cTGGGTTATCAGATTATTTAAATGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGc                                  <  1:269955/133‑1 (MQ=255)                                                                                           ggTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAAAGAACCAGACTTTCATGAGCGtt                               >  1:244214/1‑133 (MQ=255)                                                                                            gTTATCAGATTATTAAAACGGCAAAGGGCGAACTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGttt                              <  2:244214/133‑1 (MQ=255)                                                                                                tCAGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACAACACTTTCATGAGCGTTTTacc                          >  1:583108/1‑133 (MQ=255)                                                                                                  aGATTATTAAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTaccac                        >  2:523409/1‑133 (MQ=255)                                                                                                     ttattaAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTACCACcat                     >  1:74223/1‑133 (MQ=255)                                                                                                     ttattaAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTACCACcat                     >  1:23293/1‑133 (MQ=255)                                                                                                       attaAAACGGCAATGGGTGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTACCACcatca                   >  1:20414/1‑133 (MQ=255)                                                                                                                        tGAATTCAGGCATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACCTTCATGAGGGTTTTACGACCATCATGAAAGTAAATGGCCTg  >  2:162923/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                            TTGTGCCGTTGGGTGTCTGCGGAGCGGGGGATCATTTCACCGCTGAAGTTCATTACCATTGCTGAAGATATCGGGTTTATCAATGAGCTGGGTTATCAGATTATTAAAACGGCAATGGGTGAATTCAGACATTTTAGTCAGCGTGCGTCGCTGAAGGATGATTTCTTACTGCATATTAATGTTTCGCCCTGGCAGTTAAACGAACCACACTTTCATGAGCGTTTTACCACCATCATGAAAGAAAATGGCCTG  >  NC_000913/874668‑874919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑