Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	898,332	A→G	Y115C (TAC→TGC)	ybjO →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	898,332	0	A	G	100.0%	92.1 / NA	27	Y115C (TAC→TGC)	ybjO	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/10);  total (17/10)

GCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGAT  >  NC_000913/898210‑898428                                                                                                                           |                                                                                                 gCTGGTTTTCATTGAAATCTGGCGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCg                                                                                        <  1:210431/133‑1 (MQ=255)    ggTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAg                                                                                     >  2:557750/1‑133 (MQ=255)               agaTCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCAt                                                                          >  1:57730/1‑133 (MQ=255)                 aTCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTc                                                                        >  1:433865/1‑133 (MQ=255)                           cGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATc                                                              >  2:459173/1‑133 (MQ=255)                                tCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTTAGCGTTTCCGGTGTATCAAAAc                                                         >  1:237658/1‑133 (MQ=255)                                tCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAAc                                                         >  2:41409/1‑133 (MQ=255)                                   cgggTAAAAGGGCGTGGTTGGGCGCGGTGGCTATATCTGCTGACACAAACCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTg                                                      <  1:524379/131‑1 (MQ=255)                                   cTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTg                                                      <  1:41409/133‑1 (MQ=255)                                     ggTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGaa                                                    >  2:532223/1‑133 (MQ=255)                                      gTGAAGGGGCGTCGGTGGCCGCGCTGGCTATATCTGGGGACACAAACCCCCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGaaa                                                   <  2:448674/133‑1 (MQ=255)                                       tGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAAt                                                  <  1:459173/133‑1 (MQ=255)                                       tGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAAt                                                  <  2:433865/133‑1 (MQ=255)                                        gAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATc                                                 <  1:369352/133‑1 (MQ=255)                                                gTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCGCGGTGAATCAAAACTTGAAATCTTCCATAg                                         >  1:255601/1‑133 (MQ=255)                                                 tCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGc                                        >  2:141809/1‑133 (MQ=255)                                                      gggCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAt                                   >  1:525935/1‑133 (MQ=255)                                                           cgcTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGAtgctgc                              >  1:621575/1‑133 (MQ=255)                                                                        tgctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGa                 <  1:557750/133‑1 (MQ=255)                                                                          ctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGata               >  1:127516/1‑133 (MQ=255)                                                                          ctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGata               >  1:462241/1‑133 (MQ=255)                                                                          ctgACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCAATAGCCTCATGATGAAGAAGCTGCCGGata               >  1:226892/1‑133 (MQ=255)                                                                             acacAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATCCTGGAGAAGCTGCAGGATATGc            >  1:173195/1‑133 (MQ=255)                                                                              cacaAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCt           <  2:462241/133‑1 (MQ=255)                                                                               acaAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTc          >  1:462567/1‑133 (MQ=255)                                                                                   aTCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATcc      <  1:141809/133‑1 (MQ=255)                                                                                       ccgccgCAAGTTACTTGTGGGCGGCTTCGCTGGGGTGCGGTTATCCGGAGCTGTTCAGCATTCACGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGAt  >  2:116414/1‑133 (MQ=255)                                                                                                                           |                                                                                                 GCTGGTTTTCATTGAGATCTGGTGTGCGTTTTCACTGGTGAAAGGGCGTCGCTGGGCGCGCTGGCTATATCTGCTGACACAAATCACCGCCGCAAGTTACTTGTGGGCGGCTTCGCTGGGGTACGGTTATCCGGAGCTGTTCAGCATTCCCGGTGAATCAAAACGTGAAATCTTCCATAGCCTGATGCTGCAGAAGCTGCCGGATATGCTCATCCTGAT  >  NC_000913/898210‑898428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑