Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,044,474	G→T	A68D (GCC→GAC)	etp ←	phosphotyrosine‑protein phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,044,474	0	G	T	100.0%	70.1 / NA	21	A68D (GCC→GAC)	etp	phosphotyrosine‑protein phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/8);  total (13/8)

ATTGCCCAAACAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCCGGCAGACGTTTAC  >  NC_000913/1044346‑1044594                                                                                                                                 |                                                                                                                         aTTGCCCAAACAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATg                                                                                                                      <  1:324734/133‑1 (MQ=255)        aaaCAGCACTGTCTTCCCTCGGTCCTCGGGCGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCa                                                                                                               <  1:198415/133‑1 (MQ=255)         aaCAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCaa                                                                                                              >  2:381047/1‑133 (MQ=255)                        ccGCGGACCTGGGGAGCAATTGCGGTAACCTGCGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGAt                                                                                               <  2:246243/133‑1 (MQ=255)                          gcgGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTa                                                                                             <  2:74273/133‑1 (MQ=255)                                  cGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAAc                                                                                     >  1:557029/1‑133 (MQ=255)                                                        aGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCAGCAACATCTGCCGCTGTCGCATCTGCa                                                               >  1:520503/1‑133 (MQ=255)                                                        aGCAATATGTTCCGACTCCATTGCAAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCa                                                               <  2:511630/133‑1 (MQ=255)                                                                   ccGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTAc                                                    >  2:92995/1‑133 (MQ=255)                                                                    cGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCAACTGCAGGGTGTTTTAcc                                                   >  1:376438/1‑133 (MQ=255)                                                                      aCTCCATTGTCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGgtg                                                          >  2:134835/1‑124 (MQ=255)                                                                      aCTCCATTGTCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGgtg                                                          <  1:134835/124‑1 (MQ=255)                                                                      aCTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAg                                                 <  1:92995/133‑1 (MQ=255)                                                                                 aGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCAGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAacac                                      <  2:520503/133‑1 (MQ=255)                                                                                    aTCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTg                                   >  1:614133/1‑133 (MQ=255)                                                                                              aaTTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGAc                         >  1:505077/1‑133 (MQ=255)                                                                                                     tGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACg                  >  1:460169/1‑133 (MQ=255)                                                                                                     tGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACg                  >  1:410957/1‑133 (MQ=255)                                                                                                     tGCCATCTCAGCAGTGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACg                  >  2:542544/1‑133 (MQ=255)                                                                                                                   tGAGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCCGGCAGACGttt    >  1:313656/1‑133 (MQ=255)                                                                                                                     aGCTTACGTCCGTCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCCGGCAGACGTTTAc  >  2:372723/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                         ATTGCCCAAACAGCATTGTCTTCCCGCGGACCTCGGGAGCAATTGCGGTAACCTGAGCAATATGTTCCGACTCCATTGCCAGAATCAGATCGTAATTTCGTGCCATCTCAGCAGTGAGCTTACGTCCGGCATGTCCTTCAAGAGATACGCCATGATTAGCGGCAACATCTGCCGCTGTCGCATCTGCAGGGTGTTTTACCAGACCATGAACACCTGCCGATTTGACTTTTACGCCCGGCAGACGTTTAC  >  NC_000913/1044346‑1044594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑