Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,079,319	A→G	T5T (ACA→ACG)	putP →	proline:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,079,319	0	A	G	100.0%	98.6 / NA	28	T5T (ACA→ACG)	putP	proline:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/18);  total (10/18)

GCAGGATACGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCA  >  NC_000913/1079198‑1079448                                                                                                                          |                                                                                                                                  gCAGGATACGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGt                                                                                                                        <  1:14730/133‑1 (MQ=255)  cAGGATACGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTg                                                                                                                       <  1:90283/133‑1 (MQ=255)         cGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACAtttt                                                                                                                <  2:607176/133‑1 (MQ=255)         cGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACAtttt                                                                                                                <  2:580398/133‑1 (MQ=255)            tCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTtgtg                                                                                                             <  1:535877/133‑1 (MQ=255)               ccatGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCt                                                                                                          >  1:513650/4‑133 (MQ=255)                ccTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCta                                                                                                         >  1:420838/1‑133 (MQ=255)                ccTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCta                                                                                                         <  2:420838/133‑1 (MQ=255)                  tGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCtata                                                                                                       >  2:401517/1‑133 (MQ=255)                   ggTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTatat                                                                                                      <  1:28664/133‑1 (MQ=255)                    gTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATc                                                                                                     >  1:399298/1‑133 (MQ=255)                      acaacataacatCTGCTACCCCGTCCCGTTGGTAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCtt                                                                                                   <  2:288972/131‑1 (MQ=255)                          cATAAACTTTGTTACCCCTTTCCGGTGGCACATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGc                                                                                               <  1:171684/133‑1 (MQ=255)                           aTAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCTATGTTGGTGACATTTTGTGTCTATATCTTTGGCa                                                                                              <  2:429437/133‑1 (MQ=255)                                ctctGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGata                                                                                         <  2:420787/133‑1 (MQ=255)                                   tGTTACCCCGTTCCGGGGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGCCATTTTGTGTCTTTTTCTTTGGCATGATattg                                                                                      >  2:398962/1‑133 (MQ=255)                                             ttCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTa                                                                            >  2:105649/1‑133 (MQ=255)                                             ttCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTa                                                                            >  1:384425/1‑133 (MQ=255)                                                     gCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTgg                                                                    >  1:139268/1‑133 (MQ=255)                                                       aGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGACATGATAATGAGTGGGTTTATAGCCTGGCg                                                                  >  1:175942/1‑133 (MQ=255)                                                                       ttCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACttt                                                  <  1:105649/133‑1 (MQ=255)                                                                         cGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTga                                                <  2:351118/133‑1 (MQ=255)                                                                         cGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTga                                                <  2:139268/133‑1 (MQ=255)                                                                         cGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTga                                                <  1:69888/133‑1 (MQ=255)                                                                                gCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATACTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACtat                                         <  2:399298/133‑1 (MQ=255)                                                                                        taatTTTTTGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTggg                                 <  2:552510/133‑1 (MQ=255)                                                                                               ttGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGt                          <  1:398962/133‑1 (MQ=255)                                                                                                tGGAGACTTTAGATGGCTATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCAATCAACGAGAAAGTTTGACGACTATATTCTGGGCGGTCGTa                         >  2:163449/1‑133 (MQ=255)                                                                                                                 tATTAGCACGCCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTg        >  1:86918/1‑133 (MQ=255)                                                                                                                       cacgCCGATGTTGGTAACATTTCGTGTCTACATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCa  <  1:163449/133‑1 (MQ=255)                                                                                                                          |                                                                                                                                  GCAGGATACGGTCGCCCTGGTAAAACATAAACTCTGTTACCCCGTTCCGGTGGCAGATATAACGGCAAGTTTCGACATTGCCGATAATAATTTTTTGGAGACTTTAGATGGCTATTAGCACACCGATGTTGGTGACATTTTGTGTCTATATCTTTGGCATGATATTGATTGGGTTTATCGCCTGGCGATCAACGAAAAACTTTGACGACTATATTCTGGGCGGTCGTAGTCTTGGGCCATTCGTGACGGCA  >  NC_000913/1079198‑1079448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑