Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,902	T→C	C430R (TGC→CGC)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,902	0	T	C	100.0%	58.6 / NA	18	C430R (TGC→CGC)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/11);  total (7/11)

TGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGA  >  NC_000913/132777‑133010                                                                                                                              |                                                                                                             tGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTggg                                                                                                       >  1:69827/1‑133 (MQ=255)    tAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCtt                                                                                                    <  2:52585/133‑1 (MQ=255)             ccTGTGGCGTCAAAGCCATTCGTCAGGGTGCGCACTGTGAAACGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGa                                                                                           <  2:178447/133‑1 (MQ=255)                 tgGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTCGGGTTCTCGGCTGACCTg                                                                                       >  1:218668/1‑133 (MQ=255)                  ggCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTgg                                                                                      <  2:452724/133‑1 (MQ=255)                        aaaGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGGCTGCGCTTCTCGGCTGACCTGGTGATGc                                                                                >  2:212574/1‑133 (MQ=255)                        aaaGGCATTCGTCCGGGCGCGTACCGTGGACCTAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGc                                                                                <  2:218668/133‑1 (MQ=255)                         aaGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCa                                                                               <  2:448684/133‑1 (MQ=255)                                        gcgcGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCAGCTGACCTGGTGATGCAGTCTGTCTGCCacac                                                                >  2:167053/1‑133 (MQ=255)                                            gTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACcgcg                                                            <  2:69827/133‑1 (MQ=255)                                                       cGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGaa                                                 <  2:542684/133‑1 (MQ=255)                                                          aaaTGACATCTGTAGGTTCACAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGcc                                              >  2:360619/1‑133 (MQ=255)                                                                               aGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGcacca                         <  1:360619/133‑1 (MQ=255)                                                                                 gACACCACTGGCCCGATGTCCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACcaca                       <  1:263008/133‑1 (MQ=255)                                                                                    accaccGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGGGGCGTATCCGAAACCAATTGACGTGGACACCCACCACACGc                    >  2:263008/1‑133 (MQ=255)                                                                                    accaccGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGc                    <  2:518242/133‑1 (MQ=255)                                                                                      caccGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGCAGCAAGTTTACGTGAATACGCTCCACACGcag                  >  2:296964/1‑131 (MQ=255)                                                                                                      cGTGATGAGCTGAAACACGTGGAGCGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGa  <  1:256266/133‑1 (MQ=255)                                                                                                                              |                                                                                                             TGGTAGGCCGTGCCTGTGGCGTGAAAGGCATTCGTCCGGGCGCGTACTGTGAACCGAAAATGACTTCTGTAGGTTCCCAGGACACCACCGGCCCGATGACCCGTGATGAACTGAAAGACCTGGCGTGCCTGGGCTTCTCGGCTGACCTGGTGATGCAGTCTTTCTGCCACACCGCGGCGTATCCGAAGCCAGTTGACGTGAACACGCACCACACGCTGCCGGACTTCATTATGA  >  NC_000913/132777‑133010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑