| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,106,285 | T→C | F156L (TTT→CTT) | ymdB → | 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,106,285 | 0 | T | C | 96.8% | 100.9 / ‑4.3 | 31 | F156L (TTT→CTT) | ymdB | 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (18/12); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTG > NC_000913/1106165‑1106412 | gTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTAGCCGTCACGGTTTGCCCGAACAGGTAGACTTCGTCTGTTatg > 2:193063/1‑133 (MQ=255)gTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTatg > 1:20110/1‑133 (MQ=255)gTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTatg > 1:451491/1‑133 (MQ=255)gTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTCTACCCGAGCAGATAGACCTTGACTGTTatg > 1:165212/1‑133 (MQ=255) ttCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATgaaga < 2:20110/133‑1 (MQ=255) tGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAACAAAAc > 2:130368/1‑133 (MQ=255) gTTTATGGTTACCCTAGTGCGGCACCGGATGACATCGCAGTCAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGaaa < 2:165212/133‑1 (MQ=255) tATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGAc > 1:153727/1‑133 (MQ=255) tATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGAc > 2:425854/1‑133 (MQ=255) tATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATCATGAAGATAACTCCTACCTCTACGAAAGAc > 1:289130/1‑133 (MQ=255) aTGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACt < 2:398021/133‑1 (MQ=255) cccTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTAcc < 1:471198/133‑1 (MQ=255) cccTCGTGCCGCAGCAGCTGAAATCGCAGTAAGAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTAcc < 2:289130/133‑1 (MQ=255) cAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGgaga > 1:575431/1‑133 (MQ=255) cGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGatga > 2:509789/1‑133 (MQ=255) ttCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCAGCTGGCGAgcgc < 2:451491/133‑1 (MQ=255) ttattaCCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGtgctgc > 1:484547/1‑133 (MQ=255) aCCCGTCACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCGCCGGCTGGCGAGCGCGGAGCTGCCACtg > 2:224332/1‑133 (MQ=255) cACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAATGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCg > 2:518798/1‑133 (MQ=255) cACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCg > 2:142262/1‑133 (MQ=255) cACGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGACTGGCGAGCGCGGTGCTGCCACTGTGTTCg > 1:211712/1‑133 (MQ=255) cGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa < 2:538633/133‑1 (MQ=255) cGCTTTACCCGAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCa < 2:575431/133‑1 (MQ=255) cGAACAGGTATACCTTGTCTGTTATGATTAAGTAAACGCCCGCCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCTgg < 2:206837/133‑1 (MQ=255) gAACAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGt < 1:509789/133‑1 (MQ=255) aaCAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTc > 2:587826/1‑133 (MQ=255) aaCAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTc > 2:149500/1‑133 (MQ=255) aaCAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTc > 1:334568/1‑133 (MQ=255) aaCAGGTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGCTGCTGCCCCTGTGTACGCAACCTCCCGGTc > 1:206837/1‑133 (MQ=255) ggTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAgtg > 1:53639/1‑133 (MQ=255) gTATACCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAgtgt < 1:142262/133‑1 (MQ=255) tataCCTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGtgtg < 2:463465/133‑1 (MQ=255) | GTGGCTTTTCCTGCAATCAGTACTGGGGTTTATGGTTACCCTCGTGCGGCAGCGGCTGAAATCGCAGTAAAAACCGTTTCAGAATTTATTACCCGTCACGCTTTACCCGAACAGGTATACTTTGTCTGTTATGATGAAGAAAACGCCCACCTCTACGAAAGACTCCTTACCCAACAAGGAGATGAATGATTTGCCCCGGCTGGCGAGCGCGGTGCTGCCACTGTGTTCGCAACATCCCGGTCAGTGTG > NC_000913/1106165‑1106412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |