Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,140	T→C	T410A (ACT→GCT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,140	0	T	C	100.0%	101.6 / NA	29	T410A (ACT→GCT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

CAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCA  >  NC_000913/1143012‑1143268                                                                                                                                 |                                                                                                                                 cagcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACGGCGGTTCGTTGTCACGAACGGGGCCAGCACCa                                                                                                                              >  2:229282/1‑133 (MQ=255)  agcagGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAg                                                                                                                             >  2:479211/1‑133 (MQ=255)                 aTTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCGCGGTGCCAGCAGCAGAACAACACGGACaaa                                                                                                              >  1:162233/1‑133 (MQ=255)                          ggAACAATGGCGTGAACTTCCTGGATGTCCTCTTTCAGCGCATCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGAc                                                                                                     <  2:211568/133‑1 (MQ=255)                                    cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                           <  2:557430/133‑1 (MQ=255)                                    cGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCAcc                                                                                           <  2:22610/133‑1 (MQ=255)                                      tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCa                                                                                         >  1:407896/1‑133 (MQ=255)                                      tGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAAAGCGGACAAACGAGATGACTGGGTTCACCCa                                                                                         >  1:185051/1‑133 (MQ=255)                                                     ttCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgc                                                                          >  1:600253/1‑133 (MQ=255)                                                       ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcgc                                                                        >  2:93164/1‑133 (MQ=255)                                                       ctctTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGgcgc                                                                        >  1:151477/1‑133 (MQ=255)                                                              aGCGCTTCTTCTTCGCTCAGACGCCGAATAGACAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                 <  2:162233/133‑1 (MQ=255)                                                              aGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACggg                                                                 <  2:606727/133‑1 (MQ=255)                                                                   ttcttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACAtt                                                            <  1:479211/133‑1 (MQ=255)                                                                     cttcttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTc                                                          >  2:114618/1‑133 (MQ=255)                                                                        cttcGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCcag                                                       <  1:566720/133‑1 (MQ=255)                                                                             aTCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGc                                                  >  1:26585/1‑133 (MQ=255)                                                                                  aCGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGCCGCTGACGGGACATCTCCAGCCGGCCAAAg                                             >  1:251188/1‑133 (MQ=255)                                                                                     cAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCga                                          <  1:93164/133‑1 (MQ=255)                                                                                               agCGACAGCGATTCGTTGTCACGCCCGGGGCCCGCACCAGACCAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGTAGGCCAAAGCGAGAAATATGGc                                <  2:185051/133‑1 (MQ=255)                                                                                                gCGACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGATGACGGGAAATTACCAACAGGCCAACGCGACCAATATGGCt                               >  2:306591/1‑133 (MQ=255)                                                                                                  gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGTAGGCCAAAGCGAGAAATATGGCTGa                             >  1:534429/1‑133 (MQ=255)                                                                                                  gACAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGa                             >  2:354029/1‑133 (MQ=255)                                                                                                    cAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAtt                           >  2:346925/1‑133 (MQ=255)                                                                                                    cAGCGATTCGTTGTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGAtt                           >  1:330528/1‑133 (MQ=255)                                                                                                                gTCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCa               <  1:346925/133‑1 (MQ=255)                                                                                                                 tCACGCACGGTGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCAc              <  1:354029/133‑1 (MQ=255)                                                                                                                           tGCCAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACg    >  1:621337/1‑133 (MQ=255)                                                                                                                             ccAGCACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCa  <  2:407896/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                 CAGCAGGTAAGAAGCGATTGGCACAGGAACAATGGCGTGAACTTCCTGGGTGTTCTCTTTCAGCGCTTCTTCTTCGATCAGACGCAGAATAGAGAGCGACAGCGATTCGTTGTCACGCACGGTGCCAGTACCAGAACAACGCGGACAAACGTGATGACTGGATTCACCCAGTGATGGGCTCAGGCGCTGACGGGACATTTCCAGCAGGCCAAAGCGAGAAATATGGCTGATTTGAATACGCGCACGGTCCTGACGCA  >  NC_000913/1143012‑1143268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑