Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,363,657	A→C	G305G (GGA→GGC)	puuC →	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,363,657	0	A	C	100.0%	64.4 / NA	20	G305G (GGA→GGC)	puuC	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (11/9);  total (11/9)

CTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGAC  >  NC_000913/1363534‑1363780                                                                                                                            |                                                                                                                            ctggctggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGGGTGCATGGCCGGCACGCGCCTg                                                                                                                    >  1:182848/1‑133 (MQ=255)              ggcggcAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAagaga                                                                                                       >  1:515126/1‑133 (MQ=255)                         cgcCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                                                                            <  2:76330/133‑1 (MQ=255)                          gcCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATg                                                                                           <  1:403918/133‑1 (MQ=255)                                   gTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGGTGGAAGAAAGCGTCACCGATGGATTCTTAg                                                                                  >  1:185469/1‑133 (MQ=255)                                    ttttCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGc                                                                                 <  2:47215/133‑1 (MQ=255)                                         gCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAACTCTTAGc                                                                                 <  2:239673/128‑1 (MQ=255)                                         gCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAACTCTCAGc                                                                                 >  1:239673/1‑128 (MQ=255)                                                           cAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCaaa                                                          >  2:99106/1‑133 (MQ=255)                                                            aaCAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCaaaa                                                         >  2:430473/1‑133 (MQ=255)                                                                ggcggcAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCTATGAATTCTTAGCCCTTTTAAAACAGCAGGCGCAAAACTgg                                                     >  2:247084/1‑133 (MQ=255)                                                                          gcCACCGCAGGAGGCCTTTTCTACCACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGcc                                           <  2:185469/133‑1 (MQ=255)                                                                                           tttCTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCa                          <  1:430473/133‑1 (MQ=255)                                                                                            ttCTACAACCTGGGAAAGGTGTGCCTCGCCGGCACGCGCCTATTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCaa                         <  2:182848/133‑1 (MQ=255)                                                                                              cTACAACCAGGGACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAacc                       <  2:515126/133‑1 (MQ=255)                                                                                                      aGGGACAGGTTTGCATCGCCGGCATGCGCCTGTTGCTGGAATAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATggg               <  2:250079/133‑1 (MQ=255)                                                                                                        ggACAGGTGTGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCAGCCACTTGACCCCGCAACCACCATGGGCa             >  2:218379/1‑133 (MQ=255)                                                                                                              gtgtGCATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTaa       >  2:561020/1‑133 (MQ=255)                                                                                                                   taTCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGAc  >  1:446923/2‑133 (MQ=255)                                                                                                                   cATCGCCGGCACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGAc  >  1:149551/1‑133 (MQ=255)                                                                                                                            |                                                                                                                            CTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGAC  >  NC_000913/1363534‑1363780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑