BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,454,938	T→C	V24A (GTT→GCT)	paaB →	phenylacetyl‑CoA 1,2‑epoxidase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,454,938	0	T	C	100.0%	128.6 / NA	36	V24A (GTT→GCT)	paaB	phenylacetyl‑CoA 1,2‑epoxidase subunit B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/15); total (21/15)

CAGCGCTGGCCCATGCACAAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGCAACCGGAA  >  NC_000913/1454810‑1455065

cagcGCTGGCCCATGCACAAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGc                                                                                                                             <  2:414624/133‑1 (MQ=255)

   cgcTGGCCCATGCACAAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGt                                                                                                                          >  2:589493/1‑133 (MQ=255)

                          caTGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGAt                                                                                                   >  2:414292/1‑133 (MQ=255)

                              cccGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCa                                                                                               >  2:435320/1‑133 (MQ=255)

                                       tCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAAt                                                                                      >  2:310553/1‑133 (MQ=255)

                                              aGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTg                                                                               <  1:310553/133‑1 (MQ=255)

                                                gagaTTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGAt                                                                             >  2:363049/1‑133 (MQ=255)

                                                             aGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCcgtc                                                                >  2:601978/1‑133 (MQ=255)

                                                             aGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCcgtc                                                                <  1:414292/133‑1 (MQ=255)

                                                                  ggttgccTGGCCGTTATACGAAGTTTTCGTGCGTGGTAAACAGGGCTTACCCCACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACCCCCGTCGTAGc                                                           <  2:512042/127‑1 (MQ=255)

                                                                      tACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAg                                                       <  1:363049/133‑1 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGGCCGTGATGCTTACAGCCGTCGAAGCGAAgg                                                      >  1:174668/1‑133 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCcgtc                                                                <  2:112144/123‑1 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCcgtc                                                                >  1:112144/1‑123 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAgg                                                      <  2:62314/133‑1 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAgg                                                      >  2:83201/1‑133 (MQ=255)

                                                                       aCTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAgg                                                      >  1:129562/1‑133 (MQ=255)

                                                                        cTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGa                                                     >  2:92996/1‑133 (MQ=255)

                                                                           gCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGGTTCGTACACCCGTCGTAGAGAAGGATGt                                                  >  2:274333/1‑133 (MQ=255)

                                                                            ccGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGtt                                                 >  2:341367/1‑133 (MQ=255)

                                                                              gTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCa                                               <  1:351962/133‑1 (MQ=255)

                                                                              gTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCa                                               >  2:502215/1‑133 (MQ=255)

                                                                                tataCGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAAt                                             >  2:344773/1‑133 (MQ=255)

                                                                                     gAAGTTTTGGTGCGTCGCCAACGGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTggg                                        <  1:274333/133‑1 (MQ=255)

                                                                                     gAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTggg                                        <  1:37159/133‑1 (MQ=255)

                                                                                     gAAGTTTTCGCGCGTCGCAAACAGTGCTCATCCCACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTggg                                        <  2:174668/133‑1 (MQ=255)

                                                                                           ttCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGa                                  <  1:435320/133‑1 (MQ=255)

                                                                                            tCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGGCGTGGCGAGGGATGTTCAATTCTGGTGGTGaa                                 >  2:293906/1‑133 (MQ=255)

                                                                                               tgcgtgGCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGc                              <  1:344773/133‑1 (MQ=255)

                                                                                                     gCAAACAGGGCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGa                        <  1:36051/133‑1 (MQ=255)

                                                                                                            gggCTTATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAAttgtt                 <  1:83201/133‑1 (MQ=255)

                                                                                                                 tATCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTc            >  1:450495/1‑133 (MQ=255)

                                                                                                                   tCACACCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGc          <  1:626810/133‑1 (MQ=255)

                                                                                                                     acacCGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGCGACATTGTTACCTCGCaa        >  1:285937/1‑133 (MQ=255)

                                                                                                                        ccGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGCAACCg     >  2:355027/1‑133 (MQ=255)

                                                                                                                         cGCCATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGCAACCgg    >  1:405816/1‑133 (MQ=255)

                                                                                                                           ccATGCTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGCAACCGgaa  >  2:581161/1‑133 (MQ=255)

CAGCGCTGGCCCATGCACAAAAACAACATGCCCGTAAGGTCGCATAAGGAGATTCAAAATGAGTAATGTTTACTGGCCGTTATACGAAGTTTTCGTGCGTGGCAAACAGGGCTTATCACACCGCCATGTTGGCAGTTTACATGCTGCCGATGAGCGGATGGCACTGGAAAATGCCCGTGATGCTTACACCCGTCGTAGCGAAGGATGTTCAATTTGGGTGGTGAAGGCGAGTGAAATTGTTGCCTCGCAACCGGAA  >  NC_000913/1454810‑1455065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`