Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,487,050	T→G	intergenic (+87/‑185)	hrpA → / → ydcF	ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,487,050	0	T	G	100.0%	84.5 / NA	24	intergenic (+87/‑185)	hrpA/ydcF	ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/14);  total (10/14)

ATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAG  >  NC_000913/1486931‑1487175                                                                                                                        |                                                                                                                              aTTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGaa                                                                                                                  <  1:625535/133‑1 (MQ=255)  ttttGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGaa                                                                                                                  >  1:328608/1‑132 (MQ=255)  ttttGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGaa                                                                                                                  <  2:328608/132‑1 (MQ=255)   tttGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGaaaa                                                                                                                >  2:16219/1‑133 (MQ=255)        aGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACa                                                                                                           >  2:91433/1‑133 (MQ=255)                       ttAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAg                                                                                            <  2:517684/133‑1 (MQ=255)                       ttAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAg                                                                                            <  1:445266/133‑1 (MQ=255)                            ggTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGaa                                                                                       <  1:542556/133‑1 (MQ=255)                             gTTAACCCTGGTATTTGCCTGATAAAGAGAAAGCCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGaat                                                                                      <  2:209858/133‑1 (MQ=255)                             gTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGaat                                                                                      <  1:16219/133‑1 (MQ=255)                                aaCCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGaataat                                                                                   >  2:19396/1‑133 (MQ=255)                                       cTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATc                                                                            >  2:41887/1‑133 (MQ=255)                                         aTTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTa                                                                          <  1:146145/133‑1 (MQ=255)                                         aTTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTa                                                                          <  1:41887/133‑1 (MQ=255)                                                tGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCAtt                                                                   <  1:19396/133‑1 (MQ=255)                                                  aTAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTc                                                                 >  2:383984/1‑133 (MQ=255)                                                                         tttAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGc                                          >  2:457665/1‑133 (MQ=255)                                                                           tAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCgg                                        >  2:140746/1‑133 (MQ=255)                                                                                ccGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTgg                                   <  1:400379/133‑1 (MQ=255)                                                                                                           tAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCtt        >  2:532062/1‑133 (MQ=255)                                                                                                           tAAGGGTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAAGAAAAACGATTTTCTCCtt        >  2:96234/1‑133 (MQ=255)                                                                                                              gggTAAAAATGCAGGAATACAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCa     <  1:274701/133‑1 (MQ=255)                                                                                                                gTAAAAATGCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCaga   <  1:140746/133‑1 (MQ=255)                                                                                                                 tAAAAATGCACGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCagag  <  2:199746/133‑1 (MQ=255)                                                                                                                        |                                                                                                                              ATTTTGCAGGCGATGGAGCAGATTAGCGGTTAACCCTGCTATTTGCCTGATAAAGAAAAAACCCGGTAAGCATTTAGCGCCGGGTTTTTTATTAATTCTAAAACGGTAAGGGTAAAAATTCAGGAATTCAGAAAAATACAATTCTCTGCTGCAAGATGAATAATGTTTATCTACAGCATTTCCTTAAAAGATATGTCAGGCTTGCGGAGTGGCGGTTAAGGACATACGATTTCCTCCTTTCAGAG  >  NC_000913/1486931‑1487175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑