Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,511,133	T→C	R377R (CGT→CGC)	ydcR →	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,511,133	0	T	C	100.0%	94.9 / NA	29	R377R (CGT→CGC)	ydcR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/11);  total (18/11)

TTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGA  >  NC_000913/1511005‑1511260                                                                                                                                 |                                                                                                                                ttGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCt                                                                                                                             >  1:486804/1‑133 (MQ=255)           tgaGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCAct                                                                                                                   <  2:133004/133‑1 (MQ=255)            gaGTACGCTTTCCACGAGGTCAGCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCActg                                                                                                                  <  1:235413/133‑1 (MQ=255)                 cGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCg                                                                                                             >  1:502129/1‑133 (MQ=255)                 cGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACCTAAACAACGCGACTGGCAGGCACTGCTGCg                                                                                                             >  1:268728/1‑133 (MQ=255)                    tttCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTa                                                                                                          >  2:622889/1‑133 (MQ=255)                      tCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATc                                                                                                        >  2:45937/1‑133 (MQ=255)                         accaGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATctgc                                                                                                     >  1:47174/1‑133 (MQ=255)                         accaGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATctgc                                                                                                     >  2:495439/1‑133 (MQ=255)                                cACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAGCAACGCGCATGGCAGGGACTGCTGCATTATATGCCTGCGGa                                                                                              >  2:185138/1‑133 (MQ=255)                                cACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGa                                                                                              >  1:377879/1‑133 (MQ=255)                                           cTTGCGCTGGTGGATTACCTTTCCACGCGCTGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtc                                                                                   <  1:280804/133‑1 (MQ=255)                                             tGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGTTGCATTATCTGCCTGCTGAAGAGAAAATtcat                                                                                 >  2:253833/1‑133 (MQ=255)                                             tGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATtcat                                                                                 <  2:377879/133‑1 (MQ=255)                                                gcTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCATGCGGATGTGAAATTTcatcat                                                                              >  1:241239/1‑133 (MQ=255)                                                      ggATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGAc                                                                        <  2:321386/133‑1 (MQ=255)                                                        aTTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAg                                                                      <  2:486804/133‑1 (MQ=255)                                                            ccTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAGAATGCAGCATAATGACAGTGGt                                                                  >  1:162837/1‑133 (MQ=255)                                                                tCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACt                                                              >  1:504696/1‑133 (MQ=255)                                                                    cgcgCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTtct                                                          <  1:45937/133‑1 (MQ=255)                                                                    cgcgCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTtct                                                          >  2:35673/1‑133 (MQ=255)                                                                                 cgCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTc                                             >  1:606888/1‑133 (MQ=255)                                                                                 cgCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTc                                             >  1:363611/1‑133 (MQ=255)                                                                                   cccATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCTTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAcccccc                                           <  2:483615/133‑1 (MQ=255)                                                                                      aTCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGa                                        <  1:470697/133‑1 (MQ=255)                                                                                       tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg                                       <  2:47174/133‑1 (MQ=255)                                                                                       tCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCCCCCCGAg                                       <  2:606888/133‑1 (MQ=255)                                                                                            gtcgCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGtt                                  >  2:340727/1‑133 (MQ=255)                                                                                                     gTCGCCAGGTTGCGGCACCTAACCAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg                         <  1:185138/133‑1 (MQ=255)                                                                                                       cGCCAGTTTGCGGAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCg                       >  2:37379/1‑133 (MQ=255)                                                                                                                   gAACGTAAACAACGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTgg           <  1:495439/133‑1 (MQ=255)                                                                                                                            caacGCGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGa  <  1:342467/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                TTGCAGTTGATGAGTACGCTTTCCACCAGCTCACCGATGCAACTTGCGCTGGTGGATTACCTTTCCACGCGCCGATACGACGCCCATCTTCGTCGCCTGCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCGGCACTGA  >  NC_000913/1511005‑1511260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑