Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,550,077	A→G	T893A (ACG→GCG)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,550,077	0	A	G	100.0%	86.1 / NA	25	T893A (ACG→GCG)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/14);  total (11/14)

AGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTGGCGGCGGCGAAAGGC  >  NC_000913/1549952‑1550193                                                                                                                              |                                                                                                                     aGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTa                                                                                                               <  1:115344/133‑1 (MQ=255)      aTTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTc                                                                                                          <  2:80173/133‑1 (MQ=255)      aTTGAAACCCCGCTGAGCCCTAACCCGCTGGATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTc                                                                                                          <  1:215155/133‑1 (MQ=255)       ttGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCt                                                                                                         >  2:52968/1‑133 (MQ=255)             cgccgcTGGGCACTAACCCGCTGCGTCCGAACGTGGTGTCTCACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGa                                                                                                   <  2:260564/133‑1 (MQ=255)              gccgcTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAg                                                                                                  <  1:372869/133‑1 (MQ=255)              gccgcTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAg                                                                                                  <  1:44094/133‑1 (MQ=255)                    gggCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTc                                                                                            <  2:565352/133‑1 (MQ=255)                            aCCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTg                                                                                    >  1:567443/1‑133 (MQ=255)                               cGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGAc                                                                                 >  2:540093/1‑133 (MQ=255)                                 cTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCa                                                                               >  1:312212/1‑133 (MQ=255)                                    cATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGAc                                                                                 >  2:424985/1‑128 (MQ=255)                                    cATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGAc                                                                                 <  1:424985/128‑1 (MQ=255)                                         gAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCa                                                                       <  2:567443/133‑1 (MQ=255)                                                             ttgttCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCa                                                   >  2:70581/1‑133 (MQ=255)                                                                ttCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGcc                                                >  2:487832/1‑133 (MQ=255)                                                                   gTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGa                                             >  1:361090/1‑133 (MQ=255)                                                                    tCTGTATGAATAAGACGCGCTGCGTATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGaa                                            <  2:187630/133‑1 (MQ=255)                                                                    tCTGTATGAAGAAGAAGCGCTGCGCATGCGTATAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGaa                                            <  2:250245/133‑1 (MQ=38)                                                                                  aCGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAAt                              >  2:531730/1‑133 (MQ=255)                                                                                      gcTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGc                          >  1:310968/1‑133 (MQ=255)                                                                                       cTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCg                         >  1:331877/1‑133 (MQ=255)                                                                                         gCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGaa                       <  1:52968/133‑1 (MQ=255)                                                                                                 gTAAAAAAGAGCAGTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTggc               <  2:312212/133‑1 (MQ=255)                                                                                                              gTTCCCGTATGTGGGTGCGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTGGCGGCGGCGAAAGGc  <  2:331877/133‑1 (MQ=255)                                                                                                                              |                                                                                                                     AGCCGATTGAAACGCCGCTGGGCACTAACCCGCTGCATCCGAACGTGGTGTCTAACCCGGTTGTTCGTCTGTATGAACAAGACGCGCTGCGGATGGGTAAAAAAGAGCAGTTCCCGTATGTGGGTACGACCTATCGTCTGACCGAGCACTTCCACACCTGGACCAAGCACGCATTGCTCAACGCAATTGCTCAGCCGGAACAGTTTGTGGAAATCAGCGAAACGCTGGCGGCGGCGAAAGGC  >  NC_000913/1549952‑1550193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑