Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	166,957	A→C	Y743S (TAT→TCT)	mrcB →	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	166,957	0	A	C	100.0%	88.3 / NA	25	Y743S (TAT→TCT)	mrcB	peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/11);  total (14/11)

AACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTC  >  NC_000913/166842‑167084                                                                                                                    |                                                                                                                                aaCGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTa                                                                                                                >  2:575059/1‑133 (MQ=255)   cGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAAc                                                                                                              <  2:536994/133‑1 (MQ=255)        aTACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGAc                                                                                                         >  1:47529/1‑133 (MQ=255)                  tGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAAcgccg                                                                                               >  1:560011/1‑133 (MQ=255)                         aTTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATc                                                                                        >  1:38066/1‑133 (MQ=255)                                 cagcaCGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCg                                                                                >  1:609004/1‑133 (MQ=255)                                      cGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCaga                                                                           <  2:47529/133‑1 (MQ=255)                                      cGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCaga                                                                           <  2:440342/133‑1 (MQ=255)                                         tGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                        <  2:609004/133‑1 (MQ=255)                                         tGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                        <  1:383118/133‑1 (MQ=255)                                         tGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                        <  2:104108/133‑1 (MQ=255)                                         tGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCagaaga                                                                        <  2:38066/133‑1 (MQ=255)                                                   cTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGat                                                              >  2:419394/1‑133 (MQ=255)                                                    tGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGata                                                             <  1:575059/133‑1 (MQ=255)                                                     gggTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGatat                                                            >  1:81164/1‑133 (MQ=255)                                                           gCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGt                                                      >  1:3217/1‑133 (MQ=255)                                                                           gCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAAc                                      >  2:20328/1‑133 (MQ=255)                                                                           gCCGACC‑AACTGTATGGTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACt                                     >  2:573134/1‑133 (MQ=255)                                                                                            gTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                     >  2:367861/1‑133 (MQ=255)                                                                                            gTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTgg                     <  2:560011/133‑1 (MQ=255)                                                                                            gTGCCAGCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTAGACGACGGCGGCAACTGTGTTCGCAGCGGTgg                     >  2:201927/1‑133 (MQ=255)                                                                                                  gCGGGGCAATGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCg               >  1:320480/1‑133 (MQ=255)                                                                                                           tGTCGATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGcc      >  1:377913/1‑133 (MQ=255)                                                                                                               gATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTc  <  2:3217/133‑1 (MQ=255)                                                                                                               gATTTCTCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTc  <  2:320480/133‑1 (MQ=255)                                                                                                                    |                                                                                                                                AACGTAGATACCTGGTTTGCGGGCATTGACGGCAGCACGGTGACCATCACCTGGGTCGGCCGTGATAACAACCAGCCGACCAAACTGTATGGTGCCAGCGGGGCAATGTCGATTTATCAGCGTTATCTGGCTAACCAGACGCCAACGCCGCTGAATCTTGTTCCGCCAGAAGATATTGCAGATATGGGCGTGGACTACGACGGCAACTTTGTTTGCAGCGGTGGCATGCGTATCTTGCCGGTC  >  NC_000913/166842‑167084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑