Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,612,466	A→C	V262V (GTT→GTG)	glsB ←	glutaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,612,466	0	A	C	100.0%	63.7 / NA	20	V262V (GTT→GTG)	glsB	glutaminase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/11);  total (9/11)

GTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATAT  >  NC_000913/1612348‑1612593                                                                                                                       |                                                                                                                                gTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGc                                                                                                                   >  2:153196/1‑133 (MQ=255)    tCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCTgg                                                                                                                >  2:61469/1‑133 (MQ=255)    tCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCgg                                                                                                                >  1:127254/1‑133 (MQ=255)    tCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCgg                                                                                                                >  1:132635/1‑133 (MQ=255)    tCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCgg                                                                                                                >  2:589349/1‑133 (MQ=255)          aTTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGccc                                                                                                          >  2:508226/1‑133 (MQ=255)              ttCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCAcc                                                                                                      >  1:317300/1‑133 (MQ=255)                        gCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCaa                                                                                            >  2:106996/1‑133 (MQ=255)                                     gCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGtt                                                                               <  2:525189/133‑1 (MQ=255)                                      cGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTc                                                                              <  1:372737/133‑1 (MQ=255)                                      cGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTc                                                                              <  2:132635/133‑1 (MQ=255)                                        aGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCCCCCGCCAGGCAAACTCCCCCGCGTTCTg                                                                            <  2:512706/133‑1 (MQ=255)                                          ttACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGt                                                                          <  2:317300/133‑1 (MQ=255)                                             ccTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACa                                                                       <  1:508226/133‑1 (MQ=255)                                              cTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCTGGTAGCCCCCCCCGCCAGGCAAACTCCCCCGCGTTCTGGTACAt                                                                      <  1:61469/133‑1 (MQ=255)                                                   tcatcCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCAc                                                                 <  1:453023/133‑1 (MQ=255)                                                                                    aTTTCATGCGGAACAATCGCCACAATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCt                                >  1:324259/1‑133 (MQ=255)                                                                                                  aaTCGCCACAATACCGCCGCCCACGCCAGATTTCGCCGGTAGCCCCACCAGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTcacc                  <  1:248230/133‑1 (MQ=255)                                                                                                         acaATACCGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCACCGCGTTAAGTTGGCGCCCCTGCATTGGCGTCACCACTGGtt           >  2:208241/1‑133 (MQ=255)                                                                                                                cGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAat    <  1:106996/133‑1 (MQ=255)                                                                                                                cGCCACCCACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAat    <  2:324259/133‑1 (MQ=255)                                                                                                                  ccacccacGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAatat  >  1:347463/1‑133 (MQ=255)                                                                                                                  ccacccacGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAatat  >  1:121807/1‑133 (MQ=255)                                                                                                                       |                                                                                                                                GTTTCGTCAATTGTTCAAGAACGGCAATACCCGCAAGCGAGTTACCTGCATCATCCAGTTCCGGACTCCAGACAGCGATGGCCATTTCATGCGGAACAATCGCCACAATACCGCCACCAACGCCAGATTTCGCCGGTAGCCCCACCCGCCAGGCAAACTCCCCCGCGTTCTGGTACATACCACTGGTCGCCATCAGCGCGTTAATTTGCCGCGCCTGCATTGGCGTCACCACTGGTTCATCAATAT  >  NC_000913/1612348‑1612593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑