Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,631,808	C→A	A160S (GCG→TCG)	ydfJ ←	putative transporter YdfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,631,808	0	C	A	100.0%	102.6 / NA	31	A160S (GCG→TCG)	ydfJ	putative transporter YdfJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/14);  total (17/14)

TGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCAT  >  NC_000913/1631683‑1631933                                                                                                                              |                                                                                                                              tGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGTAACGGTATACGGCATCCCCACGACAGCAGt                                                                                                                        >  1:202994/1‑133 (MQ=255)  gCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCGCCACGACAGCAGtt                                                                                                                       <  2:388219/133‑1 (MQ=255)  gCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGtt                                                                                                                       <  1:25891/133‑1 (MQ=255)       cGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCctct                                                                                                                  >  2:610584/1‑133 (MQ=255)       cGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCctct                                                                                                                  >  2:563279/1‑133 (MQ=255)               tACTGTCGTTCACCTGCTCAAAGATCGGGCTTTCTTTCAGAATCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTgag                                                                                                          <  2:201685/133‑1 (MQ=255)                   gTCGTTAAGCTTCTCGAACACCTGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATg                                                                                                      <  1:286527/133‑1 (MQ=255)                   gTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATg                                                                                                      <  1:121100/133‑1 (MQ=255)                           ccTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACAt                                                                                              >  2:4475/1‑133 (MQ=255)                                tcAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAgg                                                                                         >  2:420493/1‑133 (MQ=255)                                                     aGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCa                                                                    <  1:610584/133‑1 (MQ=255)                                                         tCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGt                                                                <  1:563279/133‑1 (MQ=255)                                                               gCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAATAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCa                                                          <  2:612235/133‑1 (MQ=255)                                                                 aaCCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCg                                                            <  1:385252/129‑1 (MQ=255)                                                                 aaCCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCg                                                            >  2:385252/1‑129 (MQ=255)                                                                   ccAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCagtta                                                      >  1:596147/1‑133 (MQ=255)                                                                             aataCCATCACGATAACACTCTCCAGGAACGTCATACGCCATCCCTACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTCCCGTTGCGCTCATGGTTCCGCAGTTAGTTCCCATAg                                            >  2:567378/4‑133 (MQ=255)                                                                              aGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGATGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGc                                           >  2:409735/1‑133 (MQ=255)                                                                                aCCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAGGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCca                                         >  2:464174/1‑133 (MQ=255)                                                                                 ccATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCcac                                        >  2:68746/1‑133 (MQ=255)                                                                                  cATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCcaca                                       <  2:596147/133‑1 (MQ=255)                                                                                   aTCACGACAACACTCTCCAGGAACTGTATACGCCATCCCCATGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACaa                                      <  2:223545/133‑1 (MQ=255)                                                                                     cACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAAt                                    >  2:400884/1‑133 (MQ=255)                                                                                       cGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATga                                  <  1:78212/133‑1 (MQ=255)                                                                                          caacaCTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATgagga                               >  1:610573/1‑133 (MQ=255)                                                                                          caacaCTCGCCAGGAACGGTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCAGAGCCACGAATgagga                               >  1:223545/1‑133 (MQ=255)                                                                                                         aCGGTATACGCCATCCCCACGACATCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCtt                <  2:202994/133‑1 (MQ=255)                                                                                                            gTATACGCCATCCCCACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTAcc             >  1:39819/1‑133 (MQ=255)                                                                                                                       ccccACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGTGCCTACCTTCTGGCGCat  >  1:257802/1‑133 (MQ=255)                                                                                                                       ccccACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCat  >  1:101529/1‑133 (MQ=255)                                                                                                                       ccccACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCat  >  1:349979/1‑133 (MQ=255)                                                                                                                       ccccACGACAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCat  <  1:420493/133‑1 (MQ=255)                                                                                                                              |                                                                                                                              TGCTGTCGGTTGGTTACTGTCGTTAACCTTCTCAAAGACCGGGCTTTCTTTCAGATTCATACGCAACCAGATAGCAAAGACCATCACGACAACACTCGCCAGGAACGGTATACGCCATCCCCACGCCAGCAGTTCCTCTTTACTGAGAATGAAGAACATAAAGGCCCAGATTGCCGTTGCGCTCAAGGTTCCGCAGTTAGTTCCCATAGCCACAAATGAGGAGATAATTCCGCGCTTACCTTTTGGCGCAT  >  NC_000913/1631683‑1631933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑