Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,634,642	C→T	G82S (GGC→AGC)	tfaQ ←	Qin prophage; putative tail fiber assembly protein TfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,642	0	C	T	100.0%	120.1 / NA	53	G82S (GGC→AGC)	tfaQ	Qin prophage; putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/26);  total (27/26)

GGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTG  >  NC_000913/1634513‑1634770                                                                                                                                  |                                                                                                                                 ggAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGa                                                                                                                               <  2:44864/133‑1 (MQ=17)     cAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                                                           <  1:535380/133‑1 (MQ=17)     cAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGcc                                                                                                                           <  1:468625/133‑1 (MQ=18)        tttttCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACg                                                                                                                        >  1:116888/1‑133 (MQ=17)               gCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATaaa                                                                                                                 <  2:410021/133‑1 (MQ=18)                       aTCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTa                                                                                                         <  2:465487/133‑1 (MQ=18)                             cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGGTGGAAGCCACGTCATAAACCGTTTTACCCCga                                                                                                   >  2:237671/1‑133 (MQ=17)                             cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCga                                                                                                   >  2:521214/1‑133 (MQ=17)                                ccAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatga                                                                                                <  2:422283/133‑1 (MQ=17)                                   ggCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt                                                                                             >  1:612253/1‑133 (MQ=17)                                        tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTGATAAACCCTTTTACACCGATGCTCTTCAAc                                                                                        >  1:213689/1‑133 (MQ=11)                                        tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACATCACAAAACGTTTTACACCGACGATCTTCAAc                                                                                        >  2:239152/1‑133 (MQ=11)                                        tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTAGAAGCCACATCAAACACCGTTTTACGCCGACAATCTTCacc                                                                                        >  2:305786/1‑131 (MQ=11)                                        tgCCGTTCAACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAAc                                                                                        >  2:133949/1‑133 (MQ=17)                                         gCCGTTCCGCTTCTGGAACTCCCCTTCCGGGGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACg                                                                                       <  1:305786/133‑1 (MQ=255)                                              tCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg                                                                                  >  2:605139/1‑133 (MQ=17)                                              tCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATg                                                                                  >  2:619317/1‑133 (MQ=17)                                                  cTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCAc                                                                              >  2:7884/1‑133 (MQ=17)                                                    tCTGAAACTCCCCTTCCGGCGATAACCAGGCAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                                                            <  1:269351/133‑1 (MQ=17)                                                        aaaCTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCTATGTTCTTCAACGAGATGGCACGATGAc                                                                        >  2:269351/1‑133 (MQ=255)                                                            ttcccTTCCGGCGATAACCAGGTACCTTCTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                    <  2:295266/131‑1 (MQ=11)                                                            tCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                    <  1:598322/133‑1 (MQ=17)                                                             ccccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatc                                                                   >  2:39944/1‑133 (MQ=17)                                                             ccccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGAACTTGAACGAGATGCCACCATGACtcatc                                                                   >  2:242869/1‑133 (MQ=255)                                                              cccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                                                            >  1:539048/1‑123 (MQ=17)                                                              cccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGa                                                                            <  2:539048/123‑1 (MQ=17)                                                              cccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                                  <  2:606261/133‑1 (MQ=17)                                                               ccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatc                                                                   <  1:557607/131‑1 (MQ=17)                                                               ccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatc                                                                   >  2:557607/1‑131 (MQ=17)                                                                   ccGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGtt                                                             >  1:361496/1‑133 (MQ=17)                                                                          tAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACa                                                      <  1:605840/133‑1 (MQ=17)                                                                          tAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACa                                                      <  2:339339/133‑1 (MQ=17)                                                                                 gTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGa                                               >  1:61456/1‑133 (MQ=17)                                                                                     cATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcc                                           <  1:544847/133‑1 (MQ=17)                                                                                      aTTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca                                          <  1:7884/133‑1 (MQ=17)                                                                                           ccGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCgg                                     >  2:13215/1‑133 (MQ=17)                                                                                           ccGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAAAAGCCACGAAGCCAGCCgg                                     >  1:24721/1‑133 (MQ=17)                                                                                                    gACCGAGTTCAGAAACAAATAATGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTgg                            <  1:242869/133‑1 (MQ=12)                                                                                                      ccGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCg                          <  2:612253/133‑1 (MQ=17)                                                                                                         aGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTg                       >  1:20113/1‑133 (MQ=18)                                                                                                          gTTCAGAACTAAATACCGCGTCCCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                      <  1:170466/133‑1 (MQ=255)                                                                                                          gTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGc                      <  1:619873/133‑1 (MQ=17)                                                                                                           ttCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGGTGAAAACATCCAGGAAGCCAGCCGGGATATCTGGCGGTGCa                     >  2:170466/1‑133 (MQ=11)                                                                                                                 aataaataACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCg               <  1:33368/133‑1 (MQ=17)                                                                                                                  ataaataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCgg              <  1:39944/133‑1 (MQ=17)                                                                                                                   taaataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGt             >  2:318428/1‑133 (MQ=18)                                                                                                                   taaataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGt             >  1:411572/1‑133 (MQ=17)                                                                                                                     aataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc           <  1:133949/133‑1 (MQ=17)                                                                                                                     aataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTAc           <  2:20113/133‑1 (MQ=17)                                                                                                                          cgcgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGACGGCGCAATATCGCTACTGttt      >  1:262770/1‑133 (MQ=255)                                                                                                                            cgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGTCACGAAACCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc    >  2:147574/1‑133 (MQ=12)                                                                                                                            cgTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGc    <  1:619317/133‑1 (MQ=17)                                                                                                                             gTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGATGCCATCTCGGTACTGTTTGCt   >  1:266846/1‑133 (MQ=255)                                                                                                                              tCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTg  >  2:6746/1‑133 (MQ=11)                                                                                                                                  |                                                                                                                                 GGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTTGCTG  >  NC_000913/1634513‑1634770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑